Genetic evidence for an additional function of phage T4 gene 32 protein: interaction with ligase.

Mosig, Gisela; Breschkin, Alan

doi:10.1073/pnas.72.4.1226

Cited by 35 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Highly specific interactions also occur between DNA unwinding proteins and DNA polymerases (29,37,38,44,47). Indirect evidence suggests a further interaction between the T4 DNA unwinding protein and the T4 DNA ligase (40) and perhaps with other as yet unidentified T4 proteins (34). a See Table 1.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein)

Koch¹,

McGaw²,

Drake³

1976

J Virol

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…All base pair substitution pathways are affected. Possible physiological interactions between gene 32 and rII mutations (40) have been eliminated as causes of altered revertant frequencies of rII mutations in gene 32 ts backgrounds.…”

mentioning

confidence: 99%

Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein)

Koch¹,

McGaw²,

Drake³

1976

J Virol

View full text Add to dashboard Cite

show abstract

“…The DNA arrest phenotype of gene 59 mutants had suggested that gp59 is not required during origin initiation but is required for recombination-dependent DNA replication (see below). The 5Ј-to-3Ј exonuclease function of host DNA polymerase I can substitute for defective T4 RNase H (427) and E. coli ligase can substitute for T4 ligase, if the T4 rII genes are mutated (59,159,504,580,776 (Table 2), because these genes are important for recombination-dependent DNA replication and repair of broken forks (see below). Since DNA is constantly assaulted by intrinsic and extrinsic damaging agents, DNA replication and recombination are tightly correlated and should not be considered in isolation.…”

Section: Dna Replication Proteinsmentioning

confidence: 99%

Bacteriophage T4 Genome

Miller

Kutter

Mosig

et al. 2003

Microbiol Mol Biol Rev

703

801

View full text Add to dashboard Cite

SUMMARY Phage T4 has provided countless contributions to the paradigms of genetics and biochemistry. Its complete genome sequence of 168,903 bp encodes about 300 gene products. T4 biology and its genomic sequence provide the best-understood model for modern functional genomics and proteomics. Variations on gene expression, including overlapping genes, internal translation initiation, spliced genes, translational bypassing, and RNA processing, alert us to the caveats of purely computational methods. The T4 transcriptional pattern reflects its dependence on the host RNA polymerase and the use of phage-encoded proteins that sequentially modify RNA polymerase; transcriptional activator proteins, a phage sigma factor, anti-sigma, and sigma decoy proteins also act to specify early, middle, and late promoter recognition. Posttranscriptional controls by T4 provide excellent systems for the study of RNA-dependent processes, particularly at the structural level. The redundancy of DNA replication and recombination systems of T4 reveals how phage and other genomes are stably replicated and repaired in different environments, providing insight into genome evolution and adaptations to new hosts and growth environments. Moreover, genomic sequence analysis has provided new insights into tail fiber variation, lysis, gene duplications, and membrane localization of proteins, while high-resolution structural determination of the “cell-puncturing device,” combined with the three-dimensional image reconstruction of the baseplate, has revealed the mechanism of penetration during infection. Despite these advances, nearly 130 potential T4 genes remain uncharacterized. Current phage-sequencing initiatives are now revealing the similarities and differences among members of the T4 family, including those that infect bacteria other than Escherichia coli. T4 functional genomics will aid in the interpretation of these newly sequenced T4-related genomes and in broadening our understanding of the complex evolution and ecology of phages—the most abundant and among the most ancient biological entities on Earth.

show abstract

“…Although in E. coli it has not yet been possible to replication (23) encoded membrane proteins, namely the products of the only confer a replication defect but also an accomrIlA and rlIB genes (27). defect (Xgx) that results in blue colonies on X-Gal…”

Section: Resultsmentioning

confidence: 99%

Signal strains that can detect certain DNA replication and membrane mutants of Escherichia coli: isolation of a new ssb allele, ssb-3

Schmellik-Sandage

Tessman

1990

J Bacteriol

View full text Add to dashboard Cite

Mutations in several dna genes of Escherchia coli, when introduced into a strain with a lac fusion in the SOS gene sulA, resulted in formation of blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-1-Dgalactoside (X-Gal). Unexpectedly, several lines of evidence indicated that the blue colony color was not primarily due to induction of the SOS system but rather was due to a membrane defect, along with the replication defect, muking the cel X-Gal extrasensitive (phenotypically Xgx), possibly becaue of enhed permeability to X-Gal or leakage of J-galactosWdae. ( well-studied ssb alleles, ssb-1 and ssb-113. The technique also yielded dnaB mutants; fortuitously, uvrA mutants were also found.Although many genes involved in DNA replication and modulation of DNA structure in Escherichia coli have been identified, several replication proteins have not yet been matched with any gene (21), suggesting that other DNA replication genes, designated dna genes, remain to be identified. Furthermore, several suppressors of mutations in dna genes are known, and these suppressors may represent new dna genes (1, 39).Bacterial genes whose products are involved in DNA replication have been identified by several strategies that entail detection of mutants under nonpermissive growth conditions. Temperature-sensitive dna mutants have been selected by their resistance to UV irradiation resulting from their failure to incorporate 5-bromouracil into DNA at high temperatures (7,8). Such dna mutants have also been selected by their resistance to thymine starvation, which does not affect nonreplicating DNA (44). A variation on the same theme is to take advantage of the inability of mutants to incorporate labeled thymine at high temperatures while remaining able to synthesize protein and RNA (30, 32, 37, 38).Our isolation procedure attempts to identify bacterial genes whose products are involved in DNA replication. The method was designed to detect dna mutants by virtue of defects in the structure of their DNA, but it apparently succeeded for other reasons. number of unlinked genes collectively called the SOS regulon (28). The promoters of a number of SOS genes have been fused to the lac operon (17); these lac fusion genes produce 0-galactosidase in response to DNA damage. The presence of DNA defects in a dna mutant, particularly an excess of single-stranded DNA regions, would be expected to result in induction of an SOS fusion gene, causing a blue colony on a plate containing 5-bromo-4-chloro-3-indolyl-,-D-galactoside (X-Gal), a chromogenic substrate for P-galactosidase.Evidence that some dna mutations can indeed induce the SOS systen) comes from the work of Schuster et al. (31), although induction was obtained only at temperatures nonpermissive for growth of the dna mutants. They found that strains mutated in dnaB, dnaC, dnaE, or dnaG, when incubated for 3 h at a nonpermissive temperature and then shifted to 30°C, produced an SOS response, namely induction of prophage lambda. Mutations in dnaB produced particularly strong induction of...

show abstract

Genetic evidence for an additional function of phage T4 gene 32 protein: interaction with ligase.

Cited by 35 publications

References 43 publications

Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein)

Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein)

Bacteriophage T4 Genome

Signal strains that can detect certain DNA replication and membrane mutants of Escherichia coli: isolation of a new ssb allele, ssb-3

Contact Info

Product

Resources

About