Association of the UCP-1 single nucleotide polymorphism A-3826G with the dampness-phlegm pattern among Korean stroke patients

Abstract

BackgroundPatients with stroke have various syndromes and symptoms. Through pattern identification (PI), traditional Korean medicine (TKM) classifies the several syndromes and symptoms of stroke patients into five categories: Fire-heat (FH), Dampness-phlegm (DP), Yin-deficiency (YD), Qi-deficiency (QD) and Blood-stasis (BS). DP has been associated with obesity and hyperlipidemia. Uncoupling protein-1 (UCP-1), which plays a major role in thermogenesis and energy expenditure can increase the risk of obesity and can be related metabolic disorders. In this study, we elucidated the association of three polymorphisms located in the UCP-1 promoter and coding region with DP among Korean stroke patients.Methods1,593 patients with cerebral infarction (583/DP, 1,010/non-DP) and 587 normal subjects were enrolled. The genotypes A-3826G, G-1766A and Ala64Thr (G+1068A) for each subject were determined by polymerase chain reaction with TaqMan probes and five percent of subjects were re-genotyped by sequencing method to confirm the accuracy of genotyping. The results were analyzed using a multiple logistic regression model to evaluate the genetic associations: the UCP-1polymorphisms of normal versus those of DP subjects and those of normal versus those of non-DP subjects.ResultsA significantly higher percentage of subjects in the DP group possessed the A-3826G G allele than the A allele (OR=1.508, p=0.006). Furthermore, the number of subjects with the GG type of A-1766G was significantly lower in the non-DP group than the normal group in the recessive model (OR=0.606, p=0.042). In addition, an analysis of the relationship among 2 SNPs of UCP-1 and lipid serum concentration showed that the serum level of HDL cholesterol was significantly higher in subjects with the A-3826G G allele in the normal group (p=0.032). Serum triglyceride and HDL cholesterol were also associated with the A-1766G variant in the recessive model (p=0.002, p=0.046).ConclusionsThese results suggest that that the A-3826G and A-1766G UCP-1 polymorphisms, which are related to obesity, might be candidate genetic markers for the DP pattern in the TKM diagnosis system.

Stroke in Traditional Korean Medicine: A Nine-Year Multicentre Community-Based Study in South Korea

“…Similar studies that show the association between genetic polymorphisms and PI were performed in TKM and TCM17242526. Specifically, the polymorphisms in UCP2 and UCP3, which were significantly associated with BMI and serum cholesterol in Korean female subjects, were also associated with DP pattern242728.…”

“…These results are similar to those previously reported by Min et al . 15 , and other studies showed that DP was significantly related to the obese phenotype 16 17 18 .…”

“…Many previous studies were performed to evaluate the relationship between Western indicators, such as body composition or blood parameters, and PI patterns1516171819. Tables 3 and 5, respectively, show the distribution of body composition and blood parameters among PI.…”

The Interrelationships between Intestinal Permeability and Phlegm Syndrome and Therapeutic Potential of Some Medicinal Herbs

“…In contrast, the rs2725220 allele G was associated with hyperuricemia in patients with nonPhlegm block [ 92 ]. In a Korean study on the relationship between gene polymorphisms and Phlegm syndrome, A-3826G and A-1766G polymorphisms in the UCP-1 gene known as obesity-related polymorphisms could be used as candidate genetic markers for the Phlegm-Dampness syndrome in stroke patients [ 98 ]. A self-reported Phlegm pattern questionnaire was developed using the Delphi method, reflecting the opinions of clinical experts in the context of TEAM.…”

Recent Advances and Perspective of Studies on Phlegm Syndrome in Chinese Medicine

“…Based on the analysis of gene polymorphism of peripheral blood, Lim et al found that A-3826G and A-1766G uncoupling protein-1 polymorphisms, which are related to obesity, might be candidate genetic markers for the dampness-phlegm syndrome in patients with stroke [ 7 ]. Additionally, Ko et al found that neuropeptide Y (NPY) gene polymorphism was associated with dampness-phlegm syndrome in stroke patients.…”