Association of TLR2 gene Arg753Gln polymorphism with urinary tract infection in children

Abstract

The aim of this study is to investigate Arg753Gln allele polymorphisms of toll-like receptor-2 (TLR2) gene distribution, allele frequency in urinary tract infection (UTI) and genotype-phenotype association of TLR2 gene in children with UTI. The polymorphism was investigated in 124 children with UTI (22 boys and 102 girls; mean age 5.81 +/- 3.47 years) with direct DNA sequencing-based method. TLR2 gene Arg753Gln allele frequency was higher in the patient group when compared with control group (OR 3.14, 95%CI 1.53-6.44, P < 0.001). The frequency of the Arg753Gln allele was significantly higher in gram-positive group than in gram-negative group (OR 7.64, 95%CI 2.80-20.81, P < 0.001). The frequency of UTI was found significantly higher in the Arg753Gln allele carriers of TLR2 gene than the non-carriers (OR 4.94, 95%CI 1.09-22.33, P < 0.05). Similarly, the incidence of asymptomatic UTI was also found significantly higher in the group carrying Arg753Gln allele (OR 3.73, 95%Cl 1.54-9.04, P < 0.05). As a result, we suggest that TLR2 gene could be the predisposing factor for urinary tract infection. Additionally, we observed that subjects carrying the TLR2 Arg753Gln allele had higher risk of urinary tract infection with gram-positive pathogens, history of more than two attacks of UTI and asymptomatic UTI.

Polymorphisms in the Surfactant Protein A Gene Are Associated with the Susceptibility to Recurrent Urinary Tract Infection in Chinese Women

“…Clinical studies showed that only a fraction of r-UTI patients had obvious functional, anatomic and behavioral risk factors, which were considered to be strongly associated with an increased risk of r-UTI. Recently, genetic studies have reported the associations of polymorphisms within innate immune molecules such as Toll-like receptor 2 and 4 (TLR2 and TLR4) with r-UTI susceptibility (Karoly et al 2007;Lundstedt et al 2007;Tabel et al 2007;Hawn et al 2009). The host genetic factor has been considered as one of the pathogenesis of r-UTI.…”

Abnormal Immunological Profile and Vaginal Microbiota in Women Prone to Urinary Tract Infections

“…These findings have been taken as indicative of a genetic determinant in the susceptibility to recurrent UTI. The immunological status of UTI-prone women has not previously been well characterized, but there is some indication that genetic polymorphism related to induction of immune responses could contribute to the susceptibility (15,45). Cell-mediated innate responses (in particular, neutrophil action) form the basis of the defense against genitourinary tract infections (44).…”

Lactic Acid Bacteria in the Prevention of Urogenital and Respiratory Infections

“…Polymorphism in several genes (Interleukin -1B [IL -1B], Tumor Necrosis Factor -α [TNF -α ], TLR -2, TLR -4, etc.) may infl uence the predisposition to BV, VVC, UTI, and risk for preterm deliveries in different ethnic groups (e.g., Caucasian, black, and Brazilian women; Cauci et al 2007 ;Genc et al 2007 ;Giraldo et al 2007 ;Karoly et al 2007 ;Tabel et al 2007 ). These examples indicate the complexity of the microbial -host interactions, in which the relationships established between the probiotic bacteria and the host immune system are included.…”