Association of TNF-α 308 polymorphism with diabetes mellitus type 2

Abstract

Back ground: The association between tumors necrosis factor-alpha (TNF-á)308 polymorphism and type 2 diabetes mellitus (T2DM) remains controversial .The variation in ethnicity and life style play important role in these conflicting results.Objective: To investigate association of TNF-á 308 polymorphism with T2DM,TNF level and body mass index in these patients.Patients and methods: The current case control study included fifty patients with T2DM in addition to twenty five healthy controls. The fasting blood sugar (FBS)and fasting blood (cholesterol, triglyceride) were done by colorimetric methods .The body mass index (BMI) was calculated for each patients and healthy controls. The level TNF-á in serum was measured by ELISA method(Ray biotechnology/ USA, 46078). The TNF-á 308 polymorphism was done by restriction enzyme digestion after polymerase chain reaction (PCR).Result: The age range for T2DM patients was (43.54±4.590) year while for control was (45.04±4.394) year. The T2DM patients whom carry AA alleles for TNF-á 308 polymorphism showed highly significant association with study parameter F.B.S, BMI, cholesterol, triglyceride and TNF-á level with (P.0.01).The T2DM patients with normal allele GG genotyping and GA genotyping of TNF-á 308 polymorphism also showed highly significant association with study parameter F.B.S,BMI,Cholesterol,Triglyecride and TNF- á with (P.0.01). The TNF- á level in serum of T2DM patients showed highly significant association with F.B.S, cholesterol and triglyceride with (P P.0.01), however the TNF- á level was nonsignificant with BMI in T2DM patients.Conclusion: In the present study TNF-á 308 polymorphism allele (AA, GA) showed a statistically significant association with TNF-á level in serum of T2DM patients and BMI. The AA and GA alleles showed a statistically significant association with high fasting glucose level. The TNF- á level didn't show a statistically significant association with BMI.

Evaluation of TNF-α, Apelin and Visfatin in a sample of Iraqi patients with newly diagnosed Type2 Diabetes Mellitus

“…Additionally, the increased apelin levels among rats indicated that it had a powerful anti-type-2 diabetes activities and acted as insulin-sensitization agents [34,35]. The present study in agreement with result done by Hachim et al, who Stated Association of TNF-α 308 polymorphism with diabetes mellitus type 2 [36], another study also demonstrate by Liu et al, who said that Meta-analysis of 19 studies shows that elevated TNF-levels are strongly associated with an increased risk of developing Type 2 Diabetes Mellitus (T2DM) [37].…”