Association of Uterine and Salpingeal Fibrosis with Chlamydial Hsp60 and Hsp10 Antigen-Specific Antibodies in<i>Chlamydia</i>-Infected Koalas

Higgins, Damien P.; Hemsley, Susan; Canfield, P. J.

doi:10.1128/cdli.12.5.632-639.2005

Cited by 44 publications

(36 citation statements)

References 38 publications

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“…Transforming growth factor (TGF)-β1 and interleukin (IL)-6 induce Th17 cells, and they start secretion of IL-17A; which acts itself as profibrotic for myofibroblasts and further stimulates inflammatory cells for TGF-β1 secretion (16). Regulatory T cells a have dual function in fibrogenesis; subsets produce TGF-β1 and promote fibrosis, whereas other release immunosuppressive cytokines IL-10 (17).…”

Section: Pathogenesis Of Liver Fibrosismentioning

confidence: 99%

Delivery and Targeting of miRNAs for Treating Liver Fibrosis

Kumar

Mahato

2014

Pharm Res

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Liver fibrosis is a pathological condition originating from liver damage that leads to excess accumulation of extracellular matrix (ECM) proteins in the liver. Viral infection, chronic injury, local inflammatory responses and oxidative stress are the major factors contributing to the onset and progression of liver fibrosis. Multiple cell types and various growth factors and inflammatory cytokines are involved in the induction and progression of this disease. Various strategies currently being tried to attenuate liver fibrosis include the inhibition of HSC activation or induction of their apoptosis, reduction of collagen production and deposition, decrease in inflammation, and liver transplantation. Liver fibrosis treatment approaches are mainly based on small drug molecules, antibodies, oligonucleotides (ODNs), siRNA and miRNAs. MicroRNAs (miRNA or miR) are endogenous noncoding RNA of ~22 nucleotides that regulate gene expression at post transcription level. There are several miRNAs having aberrant expressions and play a key role in the pathogenesis of liver fibrosis. Single miRNA can target multiple mRNAs, and we can predict its targets based on seed region pairing, thermodynamic stability of pairing and species conservation. For in vivo delivery, we need some additional chemical modification in their structure, and suitable delivery systems like micelles, liposomes and conjugation with targeting or stabilizing the moiety. Here, we discuss the role of miRNAs in fibrogenesis and current approaches of utilizing these miRNAs for treating liver fibrosis.

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Section: Pathogenesis Of Liver Fibrosismentioning

confidence: 99%

Delivery and Targeting of miRNAs for Treating Liver Fibrosis

Kumar

Mahato

2014

Pharm Res

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“…Although cHsp60 clearly plays a prominent role in chlamydial pathogenesis, it is not the only molecule involved. The genetically linked protein cHsp10, encoded by the groES gene upstream of groEL-1, is also associated with disease complications (6,23,27,33). Moreover, several studies have demonstrated that genetic predisposition plays a significant role in chronic chlamydial disease (10,12,38).…”

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confidence: 99%

“…Patients with coronary artery disease often have serological evidence of previous infection by Chlamydia pneumoniae (45), and cHsp60 has been directly identified in human atheromatous tissue (31). Most recently, C. pneumoniae and Chlamydia pecorum cHsp60s have been implicated in urogenital tract disease in koalas (Phascolarctos cinereus), leading to infertility and death; chlamydiae are the most commonly recognized disease agents in the threatened koala population (23,24).…”

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confidence: 99%

Chlamydial Hsp60-2 Is Iron Responsive in Chlamydia trachomatis Serovar E-Infected Human Endometrial Epithelial Cells In Vitro

LaRue¹,

Dill²,

Giles³

et al. 2007

Infect Immun

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Chlamydial 60-kDa heat shock proteins (cHsp60s) are known to play a prominent role in the immunopathogenesis of disease. It is also known that several stress-inducing growth conditions, such as heat, iron deprivation, or exposure to gamma interferon, result in the development of persistent chlamydial forms that often exhibit enhanced expression of cHsp60. We have shown previously that the expression of cHsp60 is greatly enhanced in Chlamydia trachomatis serovar E propagated in an iron-deficient medium. The objective of this work was to determine which single cHsp60 or combination of the three cHsp60 homologs encoded by this organism responds to iron limitation. Using monospecific polyclonal peptide antisera that recognize only cHsp60-1, cHsp60-2, or cHsp60-3, we found that expression of cHsp60-2 is responsive to iron deprivation. Overall, our studies suggest that the expression of cHsp60 homologs differs among the mechanisms currently known to induce persistence.

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“…7,10,36 Together, chlamydial infections contribute not only to the overall health decline of koala populations, but also to reduced reproductive rate. 4,12,22 In addition to Chlamydia spp., a number of other bacterial organisms, including Mycoplasma spp. and Ureaplasma 770490V DIXXX10.1177/1040638718770490Multiplex PCR detection of bacterial pathogens in koalasHulse et al spp., may contribute to population decline, but their epidemiology and pathogenicity have yet to be fully characterized in the koala.…”

Section: Introductionmentioning

confidence: 99%

Development and application of two multiplex real-time PCR assays for detection and speciation of bacterial pathogens in the koala

et al. 2018

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Infectious diseases have contributed to the decline in the health of koala ( Phascolarctos cinereus) populations in the wild in some regions of Australia. Herein we report the development and validation of 2 multiplex real-time PCR (rtPCR) panels for the simultaneous detection of Mycoplasma spp., Ureaplasma spp., Bordetella bronchiseptica, and Chlamydia, including speciation and quantification of Chlamydia, in ocular, reproductive, and nasal swab samples in addition to semen and male urogenital and reproductive tissues, from koalas. Each rtPCR panel was developed for use as a single-tube reaction using pathogen-specific primers and fluorescently labeled probe sets. DNA extracted from reference strains and isolates was used for validation of sequence gene targets for the multiplex rtPCR panels. Each panel was shown to be sensitive and specific in detecting and differentiating the bacterial pathogens. The multiplex rtPCR panels were used to screen clinical samples from free-ranging and hospitalized koalas for multiple pathogens simultaneously. The multiplex rtPCR will improve turnaround time compared to individual-pathogen rtPCR methods used, to date, for confirmation of diagnosis and will provide the wildlife clinician with the ability to make treatment decisions more rapidly.

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Association of Uterine and Salpingeal Fibrosis with Chlamydial Hsp60 and Hsp10 Antigen-Specific Antibodies inChlamydia-Infected Koalas

Abstract: Infection by

Cited by 44 publications

References 38 publications

Delivery and Targeting of miRNAs for Treating Liver Fibrosis

Delivery and Targeting of miRNAs for Treating Liver Fibrosis

Chlamydial Hsp60-2 Is Iron Responsive in Chlamydia trachomatis Serovar E-Infected Human Endometrial Epithelial Cells In Vitro

Development and application of two multiplex real-time PCR assays for detection and speciation of bacterial pathogens in the koala

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