Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

Abstract

Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep.Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population.Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes.Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

Characterization and Association of CFHR5 Gene Polymorphism with Fatty Acid Composition and Cholesterol in Sheep

“…The association analysis of CFHR5 gene polymorphism and breed showed a significant association (P<0.05) fatty acid composition with tridecanoic acid (C13) i n J S and myristoleinic acid (C14:1) in BCS. The results of this study were consistent with a previous study by Harahap et al (2021), Munyaneza et al (2019), andListyarini et al (2022) with different genes named HSD17β13, BHMT, and OLFML3 respectively. Fatty acids that show significance were miristoleic acid (C14:1), ginkgoic acid (C17:1), tridecanoic acid (C13:0), and heptadecanoic acid (C17:0).…”

Polymorphism and Association of the Novel KCTD2 Gene with Flavor and Odor in Indonesian Local Sheeps

“…The prospect of sheep farming in Indonesia has the potential to be developed because in addition to meeting domestic demand it also has the opportunity to become an export commodity (Falahudin and Imanudin, 2018). Popular Indonesian breeds in the country and notable breeds include Javanese Fat-Tail sheep (JFTS), Javanese Thin-Tail sheep (JTTS) and Jonggol Sheep (JS) (Listyarini et al, 2022). Javanese Fat-Tail sheep (JFTS) are white, hornless, and is a predominant breed in East Java, whereas JTTS are small and generally white breed predominant in West Java.…”