Pendahuluan: Permasalahan gizi pada balita balita masih menjadi nomor satu di wilayah dunia, sepertihalnya stunting. Stunting dipengaruhi secara langsung oleh penyakit infeksi dan imunisasi merupakan cara untuk meningkatkan kekebalan tubuh seseorang supaya tidak terkena penyakit. Tujuan penelitian ini untuk mengetahui hubungan status imunisasi dan riwayat penyakit infeksi dengan kejadian stunting. Metode:Jenis penelitian yang digunakan adalah studi analitik dengan desain kasus-kontrol. Populasi dalam penelitian ini adalah balita yang ada diwilayah kerja Puskesmas Citarip Kota Bandung. Jumlah sampel kasus sebanyak 45 responden dan sampel kontrol sebanyak 90 responden. Teknik pengambilan sampel yang digunakan adalah purposive sampling, dengan kriteria  balita stunting dari golongan umur 24-59. Data yang dikumpulkan adalah data sekunder, dimana data tersebut dikumpulkan oleh pemegang program gizi pada saat penimbangan balita di seluruh posyandu. Analisis data menggunakan uji chi-square. Hasil:Variabel yang berhubungan dengan kejadian stunting adalah riwayat penyakit infeksi (p = 0,000), OR = 7,073 (3,174-15,758), dan variabel yang tidak berhubungan adalah status imunisasi (p = 0,056). Kesimpulan:Tidak ada hubungan yang signifikan antara status imunisasi dengan kejadian stunting pada balita. Ada hubungan yang signifikan antara riwayat penyakit infeksi dengan kejadian stunting pada balita.

Background The implementation of the National Health Insurance System (NHIS) in Indonesia has been changing the primary care physician (PCP) work condition and their job satisfaction. Objective This research aimed to explore the reasons behind PCPs’ satisfaction and dissatisfaction with job satisfaction’s aspect under the NHIS reform. Methods We conducted an exploratory qualitative study within two areas in Central Java, Indonesia, using semi-structured in-depth interviews with 34 PCPs and 19 triangulation sources. We conducted both inductive and deductive analyses by the NVivo 11. Results Most PCPs felt dissatisfied with the following aspects of the NHIS: referral system, NHIS health services standard, NHIS programmes, performance evaluation and pay-for-performance, relationship with patient and workloads. PCPs felt constrained with the referral regulation and non-specialist diagnoses, which led to dissatisfaction with performance evaluation and the pay-for-performance implementation. Furthermore, an increase in workload and conflict with patients resulted from patients’ misunderstanding the NHIS health service procedures. However, PCPs felt satisfied with the chronic disease management programme and patients’ appreciation. Conclusions This study presents the reasons behind PCPs’ satisfaction and dissatisfaction with job satisfaction’s aspect under the NHIS reform. There is a need for additional discussion among all stakeholders (Ministry of Health, Social Security Agency for Health/SSAH, primary health care and physician’s professional organizations about the non-specialist diagnoses list, performance evaluation and pay-for-performance). The government and SSAH need to improve the communication and socialization of the NHIS procedures/regulations.

Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using egg white sedimentation and percoll density gradient centrifugation technique. Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratio. The obtained data were analyzed with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the sexing methods (non sexing sperm, upper and under fraction using percoll density gradient centrifugation, upper and under fraction using egg white sedimentation technique) had very significant effect (P<0.01) on motility (64.25±3.94%; 48.55±8.28%; 53±7.93%; 56.9±8.22%; 49.75±8.19% respectively) and significant effect (P<0.05) on the ratio of X and Y sperm. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation methods for the upper fraction were 28.0±2.26%:72.0±2.26% and 77.5±1.26%:22.5±1.26%. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation technique for the under fraction were 69.0±15.35%: 26.0±4.37% and 22.9±1.44%: 77.1±1.44%. The study concludes that the sexed with egg white sedimentation technique is better than percoll density gradient centrifugation on sperm motility and ratio of X and Y sperm.

Tanaman kunyit secara tradisional sudah sejak lama banyak digunakan sebagai tanaman obat. Negara India dan China menggunakan tanaman kunyit untuk mengobati penyakit empedu, selesma, batuk, diabetes, rematik, sinusitis, penyakit kulit, infeksi parasit, inflamasi, dan biliary disorders. Potensi tanaman kunyit sebagai tanaman obat menimbulkan ide untuk melakukan penelitian yang bertujuan mengetahui kandungan senyawa pada serbuk rimpang kunyit yang berpotensi sebagai antioksidan. Penelitian dilakukan dengan menganalisis kandungan senyawa pada serbuk rimpang kunyit (turmeric powder) menggunakan metode LC-MS dan dilanjutkan dengan menganalisis senyawa yang terkandung pada serbuk rimpang kunyit yang berpotensi sebagai antioksidan dengan metode in silico. Hasil penelitian menunjukkan bahwa terdapat 49 senyawa aktif yang ditemukan pada serbuk rimpang kunyit, kurkumin adalah senyawa dengan konsentrasi paling tinggi dibandingkan dengan senyawa yang lain yaitu sebesar 7,798%. Sedangkan senyawa yang berpotensi sebagai antioksidan berdasarkan analisis in silico diperoleh 11 senyawa yaitu Ascorbic acid, Quercetin, β Carotene, Arabinose, Bis Demethoxycurcumin, Demethoxycurcumin, Curcumin, Caffeic acid, Cinnamic acid, Letestuianin A, dan Calebin A. Kata kunci: turmeric powder, kurkumin, LC-MS, in silico

ABSTRAKLipoprotein lipase (LPL) merupakan enzim kunci dalam metabolisme dan transport lipoprotein yang mempengaruhi level trigliserida darah. LPL mengontrol partisi triasilgliserol antara jaringan lemak dan otot serta dapat meningkatkan penyimpanan lemak atau menyediakan energi dalam bentuk asam lemak untuk pertumbuhan otot. Penelitian ini bertujuan untuk mengeksplorasi keragaman gen LPL pada domba lokal Indonesia dan hubungannya dengan kualitas marbling. Total 66 DNA genom (260 bp) domba lokal Indonesia yang terdiri atas domba ekor tipis sumatera (50 ekor) dan domba garut (16 ekor) digunakan dalam penelitian ini. Amplifikasi DNA genome menggunakan Polymerase Chain Reaction dan metode direct sequencing digunakan untuk mengidentifikasi keragaman sekuens. Hasil sekuens dianalisis menggunakan software Bioedit dan MEGA 5.2. Sekuens kemudian disejajarkan menggunakan metode Clustal W dengan gen bank X.68308.1. Assosiasi keragaman gen LPL dengan kualitas marbling dianalisis menggunakan metode ANOVA satu arah dan uji lanjut menggunakan uji beda nyata terkecil. Hasil penelitian menemukan 3 SNPs baru pada posisi basa g.26>C/G, g.27>G dan c.192 T>C pada domba garut dan insersi g.26>C/G pada domba ekor tipis sumatera. Keragaman gen LPL posisi basa c.192 pada domba garut berasosiasi dengan asam lemak heneikosanoat, dimana genotipe TT (0.04%) memiliki kandungan lebih tinggi dibandingkan genotipe CC (0,03%) dan CT (0,02%). Kata kunci: gen LPL, SNPs, lamb, asam-asam lemak, henekosanoat ABSTRACTLipoprotein lipase (LPL) is a key enzyme that plays in metabolism and transport lipoprotein and therefore has an influence on blood triglyceride levels. LPL controls triacylglycerol partitioning between adipose tissue and muscle that increases fat storage or provides energy in the form of fatty acids for muscle growth. The research was aimed to explore Single Nucleotide Polymorphisms of LPL gene and to associate SNP with marbling quality. A total of 66 genomic DNAs consisted of sumatera thin-tail ed sheep (50 heads) and garut sheep (16 heads) were used in this study. Polymerase Chain Reaction was used to amplify genomic DNA and direct sequencing method was to identify polymorphism sequences. The sequences were analyzed with Bio Edit and MEGA 5.2. The BLAST sequence was obtained from gene bank X.68308.1. The association between the genotype and marbling quality was analyze by one way ANOVA and further between mean differences were tested using least sgnificant difference. The results showed that 3 novel SNPs i.e. insertion g.26>C; insertion g.27> G and c.192T>C on garut sheep and a SNP insertion g.26>C/G on sumatera thin-tail ed sheep. The diversity of LPL gene at c.192T>C was associated with heneicosanoic acid, whereas TT genotype (0.04%) was higher than CC (0.03%) and CT (0.02%).