Association study of childhood obesity with eight genetic variants recently identified by genome-wide association studies

Abstract

Background:Being overweight or obese is becoming increasingly common in low-and middle-income countries. The present study aimed to examine association of eight genetic variants with obesity and to estimate the cumulative effects of these variants in Chinese children. Methods:We conducted the case-control study in a total of 2,030 subjects. Genotyping of seven novel variants was performed with matrix-assisted laser desorption ionization time of flight mass spectrometry, while rs9939609 was genotyped with tetra-primer amplification refractory mutation system analysis. results: The association of two fat mass and obesityassociated gene (FTO) single-nucleotide polymorphisms (SNPs; rs9939609 and rs62048402) with body mass index (BMI) or obesity reached nominal significance at P < 0.05. We found a cumulative effect of five SNPs on the risk of overweight and obesity (odds ratio (OR) = 1.197, 95% confidence interval (CI) = 1.068-1.342, P = 0.002). Subjects carrying 9 or more effect alleles had a 127% increased risk of overweight and obesity (OR = 2.270, 95% CI = 1.403-3.671, P = 0.001) compared with subjects who carry 6 or fewer effect alleles. conclusion: We confirmed two FTO SNPs (rs62048402 and rs9939609) had nominal significant effects on BMI or obesity. We identified the cumulative effect of five SNPs on risk of overweight and obesity. The results provided evidence for identifying genetic factors related to childhood obesity.

Association Study of Three Gene Polymorphisms Recently Identified by a Genome-Wide Association Study with Obesity-Related Phenotypes in Chinese Children

“…Genotyping of the three SNPs were carried out with the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS, Sequenom). The detailed genotyping process has been described previously [19,20]. The call rates of the three SNPs were more than 99.9%.…”

“…Many replication studies for these loci have been conducted in multiple ethnic populations, including Chinese [15,16,17,18,19,20]. Two recently published studies by Wang et al [19] and Meng et al [20] from our research group confirmed some loci identified by GWA studies to be associated with obesity in Chinese children, and identified ethnic difference of effect size for some loci (supplemental table 1, available at http://content.karger.com/ProdukteDB/produkte.asp?doi=471487 ). …”

Physical activity and sedentary behavior can modulate the effect of the PNPLA3 variant on childhood NAFLD: a case-control study in a Chinese population

“…As described before [14], the CPOOA subjects were recruited from children aged 7–18 years old in 5 elementary and middle schools of Haidian District of Beijing, comprising 637 overweight or obese children and 456 normal-weight children. All the obese individuals in the selected schools were recruited with their voluntary participation.…”

The Association Between Living Environmental Factors and Adolescents’ Body Weight

“…In addition to the built environment factors, other factors, including parental excessive BMI, family income, being male, and barbeque food consumption, were found associated with young adolescents' excessive body weight, which were consistent with previous ndings. 12,[32][33][34] For example, genetic factors in uenced fat distribution as well as daily energy expenditure, energy intake, and habitual physical activities. 12 It is also speculated that families with higher incomes could have more access to healthy food and live healthier lifestyles.…”