Association study of genetic variants at single nucleotide polymorphism rs109231409 of mannose-binding lectins 1 gene with mastitis susceptibility in Vrindavani crossbred cattle

Asaf, V. N. Muhasin; Bhushan, Bharat; Panigrahi, Manjit; Dewangan, Prashant; Kumar, Amod; Kumar, Pushpendra; Gaur, G. K.

doi:10.14202/vetworld.2014.807-810

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…The results from this study differs with the earlier reports of Wang et al [6]; Liu et al [9]; Yuan et al (2013) and Asaf et al [10,11].These differences might be due to differences in target region of the gene, genetic variation between the species and can also be due to small population size in the present study . However, the SNPs and haplotypes described in our study were novel and have not been reported till date.…”

Section: Ethical Approvalcontrasting

confidence: 99%

“…In Chinese Holstein cattle, a total of 5 SNPs have been reported for MBL1 gene, and the combined genotype ATGGC/ACAAC exhibited the lowest, thus MBL1 gene possibly contributes to bacterial infection resistance and was proposed as a molecular marker of milk production traits to control mastitis [9]. In Vrindavani crossbred cattle, SNPs have been reported to be polymorphic in both the affected as well as unaffected groups of cows but no significant association has been found with the clinical mastitis [10,11]. Three SNPs at (c.1252G>A in intron1, c.2534G>A and c.2569T>C in exon2) have been reported in MBL1 gene.…”

Section: Introductionmentioning

confidence: 99%

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Association Analysis of MBL1 Gene SNPs, Genotype and Haplotypes with Clinical Mastitis in Murrah Buffaloes

Shergojry

Gupta

Mir

2021

JEAI

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Mannose-binding lectins (MBL1) gene a most important constituent of immune response system of an organism, the primary role of this gene in the classical and lectin-activation pathways is to provide protection against various dieases or bacterial pathogens. In this study, eight novel SNPs identified in the promoter region of MBL1 gene were 154C>T, 235G>A, 252A>T, 265C>A, 268T>C, 282G>A, 431G>A and 551C>G, when compared to reference sequence of Bubalus bubalis (NCBI accession number KC415281). Genomic DNA isolated from 200 lactating Murrah buffaloes was amplified for the targeted sequence of MBL1. PCR products were custom sequenced, edited and used for further analysis. The results indicated that only 551C>G polymorphism locus met Hardy–Weinberg equilibrium (c2 (2df) = 4.4: P=0.11). Pair linkage disequilibrium analysis and haplotype construction of MBL1 gene were performed using SHEsis software. Pair linkage disequilibrium analysis revealed moderate to strong linkage disequilibrium between the eight SNPs loci A total of 17 haplotypes were generated from eight SNPs in promoter region of MBL1 gene. Association between eight SNPs of MBL1 gene in Murrah buffalo has been analyzed and relative risks of the alleles for clinical mastitis were estimated with an odds ratio. Allelic association analysis showed that T allele of 154C>T, A allele of 235G>A, A allele of 252A>T, A allele of 265C>A , T allele of 268T>C, A allele of 282G>A , A allele of 431G>A and G allele of 551C>G had significant association with increased risk of clinical mastitis (P< 0.01). Haplotype analysis showed that Hap15 (CAAATAAG) and Hap16 (TAAATAAG) i.e. the haplotypes containing all 8 “at-risk” alleles, were significantly associated with clinical mastitis (P< 0.01). Hap6 (CGAATAAC) and Hap8 (CGTCCGGC) were significantly associated with a lower risk of clinical mastitis in Murrah buffaloes (P< 0.01).On the other hand, the Hap6 (CGAATAAC) and Hap8 (CGTCCGGC) which are unrepresented in affected group and without “at-risk” alleles were significantly associated with a lower risk for clinical mastitis (P<0.01).These findings indicated a MBL1 gene polymorphism could be used as genetic marker for mastitis resistance in Murrah buffaloes.

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Section: Ethical Approvalcontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association Analysis of MBL1 Gene SNPs, Genotype and Haplotypes with Clinical Mastitis in Murrah Buffaloes

Shergojry

Gupta

Mir

2021

JEAI

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“…Asaf et al . [ 56 ] in their work on MBL-1 (Chromosome 28) with PCR-RFLP claimed no significant association between SNP “rs109231409” with mastitis tolerance or susceptibility in Vrindavani crossbred cattle. Further, Zhao et al .…”

Section: Immuno-pathogenesismentioning

confidence: 99%

Molecular markers for resistance against infectious diseases of economic importance

et al. 2017

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Huge livestock population of India is under threat by a large number of endemic infectious (bacterial, viral, and parasitic) diseases. These diseases are associated with high rates of morbidity and mortality, particularly in exotic and crossbred cattle. Beside morbidity and mortality, economic losses by these diseases occur through reduced fertility, production losses, etc. Some of the major infectious diseases which have great economic impact on Indian dairy industries are tuberculosis (TB), Johne’s disease (JD), mastitis, tick and tick-borne diseases (TTBDs), foot and mouth disease, etc. The development of effective strategies for the assessment and control of infectious diseases requires a better understanding of pathogen biology, host immune response, and diseases pathogenesis as well as the identification of the associated biomarkers. Indigenous cattle (Bos indicus) are reported to be comparatively less affected than exotic and crossbred cattle. However, genetic basis of resistance in indigenous cattle is not well documented. The association studies of few of the genes associated with various diseases, namely, solute carrier family 11 member 1, Toll-like receptors 1, with TB; Caspase associated recruitment domain 15, SP110 with JD; CACNA2D1, CD14 with mastitis and interferon gamma, BoLA-DRB3.2 alleles with TTBDs, etc., are presented. Breeding for genetic resistance is one of the promising ways to control the infectious diseases. High host resistance is the most important method for controlling such diseases, but till today no breed is total immune. Therefore, work may be undertaken under the hypothesis that the different susceptibility to these diseases are exhibited by indigenous and crossbred cattle is due to breed-specific differences in the dealing of infected cells with other immune cells, which ultimately influence the immune response responded against infections. Achieving maximum resistance to these diseases is the ultimate goal, is technically possible to achieve, and is permanent. Progress could be enhanced through introgression of resistance genes to breeds with low resistance. The quest for knowledge of the genetic basis for infectious diseases in indigenous livestock is strongly warranted.

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“…However, AACCEE genotype associated with the lowest SCS was favorable for the mastitis resistance. Asaf et al 2014 reported that single nucleotide polymorphism rs109231409 located on mannose-binding lectins 1 (MBL1) gene was not associated with mastitis tolerance/susceptibility. , reported polymorphism of the BRCA1 SNPs, i.e., G22231T but reported no significant association with mastitis susceptibility in Vrindavani cattle by PCR-RFLP.…”

Section: Mastitismentioning

confidence: 99%

Candidate Gene Polymorphism vis-a-vis Immune Response to Important Infectious Diseases in Bovines

Kumar¹,

Singh²,

Chauhan³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Association study of genetic variants at single nucleotide polymorphism rs109231409 of mannose-binding lectins 1 gene with mastitis susceptibility in Vrindavani crossbred cattle

Cited by 10 publications

References 23 publications

Association Analysis of MBL1 Gene SNPs, Genotype and Haplotypes with Clinical Mastitis in Murrah Buffaloes

Association Analysis of MBL1 Gene SNPs, Genotype and Haplotypes with Clinical Mastitis in Murrah Buffaloes

Molecular markers for resistance against infectious diseases of economic importance

Candidate Gene Polymorphism vis-a-vis Immune Response to Important Infectious Diseases in Bovines

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