Association Study of ARL15 and CDH13 with T2DM in a Han Chinese Population

Abstract

Several studies indicate that plasma adiponectin levels are associated with the risk of type 2 diabetes mellitus (T2DM) or T2DM risk factors in diverse populations. In addition to the adiponectin gene, several other genes have been postulated to influence plasma adiponectin levels. In this study, we investigated two single nucleotide polymorphisms (SNPs), rs4311394 and rs4783244, located intronically in the ADP-ribosylation factor-like protein 15 (ARL15) and the T-cadherin (CDH13) genes, respectively. These SNPs were detected in a Han Chinese population using a TaqMan assay and evaluated for association with T2DM as well as with individual metabolic traits. Allele frequencies for rs4311394 were significantly different in T2DM and nondiabetes (NDM) groups (χ² = 4.49, P = 0.034). However, neither allele nor genotype frequencies for rs4783244 were associated with T2DM (χ² = 0.33, P = 0.56 and χ² = 2.35, P = 0.31 respectively). The SNPs did not exhibit significant association with individual metabolic traits in the T2DM and NDM groups. Our results indicated that the G allele of the rs4311394 might be a susceptibility factor for T2DM in the Han Chinese population (odds ratio: 1.20; 95% confidence interval: 1.01-1.41).

Association between single nucleotide polymorphisms (SNPs) in the gene of ADP-ribosylation factor-like 15 (ARL15) and type 2 diabetes (T2D) in Korean Chinese population in Yanbian, China

“…This study also showed that in Yanbian Korean Chinese population, a subject with genotype GG for SNP rs26770 in ARL15 would manifest higher risk for T2D development with conspicuous traits of recessive inheritance. But we did not find the polymorphisms of alleles on rs4311394 are related to T2D development as do in Richards et al's research on Europeans and Li et al's research on Yunnan Han Chinese population [5,6]. The difference may be due to ethnic specificity.…”

“…Even though its pathogenesis is still not completely understood, but the insulin resistance and its relative scarcity are surely primary causes for hyperglycemia. In 2009, a study with genome-wide association studies (GWAS) on the single nucleotide polymorphisms (SNPs) which are located in the gene of ADPribosylation factor-like 15 (ARL15) by Richards et al showed that the all ele rs4311394G is highly related to hypoadiponectinemia and T2D with coronary artery disease (CAD) on Europeans [5] and in 2014, Li et al found the same allele is associated with T2D development [6]. These previous studies manifest polymorphisms of ARL15 may play important roles in T2D development and its complications.…”

Genetic variants of CDH13 determine the susceptibility to chronic obstructive pulmonary disease in a Chinese population

“…However, given the high frequencies of the G allele at rs4783244 (70.7%) and the C allele at rs12922394 (73.4%) related to the elevated levels of adiponectin in control subjects, the effects of the genotypes on the susceptibility of COPD were limited. The frequency of the T allele at rs4783244 was 29.3% in our control subjects, which appears to be lower than the 36% in non-diabetic Han Chinese subjects [30] . This might be attributable to the differences between the two studies in subjects' age, gender, and smoking history.…”

“…This might be attributable to the differences between the two studies in subjects' age, gender, and smoking history. Compared to the non-diabetic subjects in Li's study [30] , our control subjects are older (65±8 years vs 50±11 years), more likely to be male (88% vs 63%), and heavier smokers. Indeed, T allele frequency is approximately 29% in Han Chinese in HapMap project data, which is comparable to our results.…”

Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex

“…During our systematic study of Arl group small G proteins, we focused on Arl15 due to its genetic implication in human diseases (Corre et al, 2018;Danila et al, 2013;Glessner et al, 2010;Li et al, 2014;Negi et al, 2013;Replication et al, 2014;Richards et al, 2009;Ried et al, 2016;Sun et al, 2015;Willer et al, 2013).…”