Association study of polymorphisms inside the miR-1657 seed region with chicken growth and meat traits

Li, H.; Sun, Guirong; Lv, Shi-jie; Wei, Yuanyuan; Han, Ruili; Tian, Yadong; Kang, Xin

doi:10.1080/00071668.2012.750716

Cited by 23 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The T/C mutation in miR-27a (?18) gene was found to be associated with litter size [31]. Previous studies showed that SNPs in chicken mature miR-1657 and miR-1614 gene were significantly associated with some economic traits, such as body weight and meat traits [32,33]. In current study, the effect of the miR-1596-SNP on other traits was not significant.…”

Section: Discussioncontrasting

confidence: 76%

Association study of a common genetic variant in pre-miR-1596 with chicken performance traits

et al. 2014

View full text Add to dashboard Cite

Increasing reports have verified that miRNAs had an important effect on the growth and development in farm animals. To evaluate the possible effect of miR-1596 polymorphisms on chicken economic traits, directly sequencing and polymerase chain reaction-restriction fragment length polymorphism, association analysis as well as online software were used. The results showed that a C > T polymorphism existed in the miR-1596 gene of the Gushi × Anka F2 resource population. The association analysis showed that it was significantly relevant with the potential of hydrogen of leg muscle, fat content of dry sample and fat content of fresh sample, shank length at 0 day and 4 weeks of age; leg weight, leg muscle weight, and breast muscle weight (P < 0.05); and highly significant association with shank girth at 8 weeks of age and abdominal fat weight (P < 0.01). We predicted the secondary structure of Gallus gallus-miR-1596 (gga-miR-1596) and the free energy by using M-fold, which were not altered. MiR-1596 is conserved between chicken and turkey. Our data implied that miR-1596 might participate in regulating the muscle development and adipogenesis.

show abstract

Section: Discussioncontrasting

confidence: 76%

Association study of a common genetic variant in pre-miR-1596 with chicken performance traits

et al. 2014

View full text Add to dashboard Cite

show abstract

“…miRNAs regulate gene expression through binding to the specific sites in the 3'UTR of their target mRNAs. The sequence of the 2-8 nt of the 5'end in mature miRNA, known as the "seed region", plays a critical role in miRNA binding and interacting with target mRNAs (Brennecke et al 2005;Li et al 2012). It has been confirmed that miRNAs can repress the expression of target mRNAs at a post-transcriptional regulation pattern in animals (Bartel 2009).…”

mentioning

confidence: 96%

MicroRNA-101-2-5p targets the ApoB gene in the liver of chicken (Gallus Gallus)

Zheng

et al. 2017

Genome

View full text Add to dashboard Cite

Apolipoprotein B (ApoB) is a major protein component of plasma lipoproteins. It is involved in many important biological processes such as lipid transportation, enzyme activity regulation, and receptor recognition. Extensive studies have shown that the expression of ApoB is regulated at multiple levels. However, the regulation of ApoB expression by microRNAs (miRNAs) still remains unknown. In the present study, identified are miRNAs that are predicted to interact with ApoB in chicken. The predicted relationship between the identified miRNAs and ApoB was verified through dual luciferase reporter assay in chicken DF1 cells, and the effect of miRNAs on ApoB expression was analyzed in chicken embryo hepatocytes stimulated by 17β-estradiol. The results show that miR-101-2-5p was predicted to interact with ApoB. Dual luciferase reporter assay together with the miR-101-2-5p mimics study demostrate that ApoB is the target of miR-101-2-5p, which suppresses the expression of ApoB through binding with the 3′UTR of ApoB. Our experiments suggest that miR-101-2-5p might be involved in lipid metabolism through binding to the 3′UTR of ApoB in the liver of egg-laying chickens.

show abstract

“…To identify SNPs that may naturally occur in lncLTR and investigate the potential biological effect of the SNPs on various traits in chicken, an F 2 population of 860 chickens generated from a cross of Gushi chickens (a slow-growing local chicken) and Anka chickens (a fast-growing broiler) as previously described [40,41] was used for the SNP scanning and association analysis in this experiment. All the chickens were managed under the same environment with free access to feed and water, and they were slaughtered at 12 weeks of age.…”

Section: Snp Detection and Association Analysismentioning

confidence: 99%

Transcriptome Profile Analysis Reveals an Estrogen Induced LncRNA Associated with Lipid Metabolism and Carcass Traits in Chickens (Gallus Gallus)

Yang

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Accumulating evidences have demonstrated that long noncoding RNAs (lncRNA) play important roles in hepatic lipid metabolism in mammals. However, no systematic screening of the potential lncRNAs in the livers of laying hens has been performed, and few studies have been reported concerning the effects of the lncRNAs on lipid metabolism in the livers of chickens during egg-laying period. The purpose of this study was to compare the difference in lncRNA expression in the livers of pre-laying and peak-laying hens at the age of 20 and 30 weeks old by transcriptome sequencing and to investigate the interaction networks among lncRNAs, mRNAs and miRNAs. Moreover, the regulatory mechanism and biological function of lncLTR, a significantly differentially expressed lncRNA in the liver between pre- and peak-laying hens, was explored in vitro and in vivo. Methods: Bioinformatics analyses were conducted to identify the differentially expressed (DE) lncRNAs between the two groups of hens. The target genes of the DE lncRNA were predicated for further functional enrichment. An integrated analysis was performed among the DE lncRNA datasets, DE mRNAs and DE miRNA datasets obtained from the same samples to predict the interaction relationship. In addition, in vivo and in vitro trials were carried out to determine the expression regulation of lncLTR, and polymorphism association analysis was conducted to detect the biological role of ncLTR. Results: A total of 124 DE lncRNAs with a P-value ≤ 0.05 were identified. Among them, 44 lncRNAs including 30 known and 14 novel lncRNAs were significant differentially expressed (SDE) with FDR ≤ 0.05. Thirty-two lncRNAs were upregulated and 12 were downregulated in peak-laying group compared with pre-laying group. The functional enrichment results revealed that target genes of some lncRNAs are involved in the lipid metabolism process. Integrated analysis suggested that some of the genes involved in lipid metabolism might be regulated by both the lncRNA and the miRNA. In addition, an upregulated lncRNA, designated lncLTR, was demonstrated to be induced by estrogen via ERβ signaling. The c242. G>A SNP in lncLTR was significantly associated with chicken carcass weight, evisceration weight, semi-evisceration weight, head weight, double-wing weight, claw weight traits, and blood biochemical index, especially for the blood triglyceride content. Conclusion: A series of lncRNAs associated with lipid metabolism in the livers of chickens were identified by transcriptome sequencing and functional analysis, providing a valuable data resource for further studies on chicken hepatic metabolism activities. LncLTR was regulated by estrogen via ERβ signaling and associated with chicken carcass trait and blood triglyceride content.

show abstract

Association study of polymorphisms inside the miR-1657 seed region with chicken growth and meat traits

Cited by 23 publications

References 31 publications

Association study of a common genetic variant in pre-miR-1596 with chicken performance traits

Association study of a common genetic variant in pre-miR-1596 with chicken performance traits

MicroRNA-101-2-5p targets the ApoB gene in the liver of chicken (Gallus Gallus)

Transcriptome Profile Analysis Reveals an Estrogen Induced LncRNA Associated with Lipid Metabolism and Carcass Traits in Chickens (Gallus Gallus)

Contact Info

Product

Resources

About