Associations between altered vitellogenin concentrations and adverse health effects in fathead minnow (Pimephales promelas)

Thorpe, Karen L.; Benstead, Rachel; Hutchinson, Thomas H.; Tyler, Charles R.

doi:10.1016/j.aquatox.2007.08.012

Cited by 71 publications

(57 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Considering that the fish receive the food continuously, any oestrogenic activity of the fish diet is likely to affect the baseline VTG concentration measured in male fish and reduce the sensitivity of experiments designed to assess the oestrogenicity of chemicals. As male fish lack the ability to remove VTG from their blood via uptake into oocytes, VTG concentrations remain high in the plasma for many weeks after exposure [1,35,36]. This probably explains why there was only a very slow reduction in the plasma VTG concentration after ceasing to feed the fish with oestrogenic food.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Beresford

Brian

Runnalls

et al. 2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Abstract-Vitellogenin (VTG) is a precursor of egg-yolk protein and is therefore present at high concentrations in the plasma of female fish. In male fish, VTG concentrations are usually undetectable or low, but can be induced upon exposure to oestrogenic substances either via the water or the diet. This work was carried out to determine the reason for apparently elevated VTG concentrations in unexposed stock male fathead minnow maintained in our laboratory. The results showed clearly that some of the food given to the fish was oestrogenic and that replacement of this with non-oestrogenic food led to a significant reduction in the basal VTG levels measured in male fish after a 6 month period. This reduction in male VTG concentrations drastically increased the sensitivity of the VTG test in further studies carried out with these fish. Moreover, a review of published concentrations of VTG in unexposed male fathead minnow suggests that this problem may exist in other laboratories. The fathead minnow is a standard ecotoxicological fish test species and so these findings will be of interest to any laboratory carrying out fish tests on endocrine disrupting chemicals.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Vitellogenin (VTG) is a precursor of egg-yolk protein, present in high concentrations (typically from 1 -15 mg/ml [1][2][3]) in the plasma of sexually mature female fish. It is produced in the liver in response to oestrogens from the ovaries.…”

Section: Introductionmentioning

confidence: 99%

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Beresford

Brian

Runnalls

et al. 2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…Besides the mortality observed (43%), a delay in the development can, for instance, increase the risk for predation in natural systems since larvae are unable to escape before the swim-up (Stalter et al, 2010b). Moreover, changes in VTG concentrations in fish can be an indicator for an effect on their reproduction system (Miller et al, 2007;Thorpe et al, 2007). Therefore, effluents from conventional WWTPs can have a significant impact on salmonid fish in natural environments in the case of low dilution of the effluent.…”

Section: Toxicity Of the Biologically Treated Effluent And Possible Imentioning

confidence: 99%

Treatment of micropollutants in municipal wastewater: Ozone or powdered activated carbon?

Margot

Kienle

Magnet³

et al. 2013

Science of The Total Environment

684

404

View full text Add to dashboard Cite

Highlights• Micropollutants are efficiently removed by both ozone and powdered activated carbon• Specific substances were removed more efficiently by ozone• Powdered activated carbon effectively removed a wider range of pollutants• Both treatments significantly reduced the toxicity of WWTP effluent AbstractMany organic micropollutants present in wastewater, such as pharmaceuticals and pesticides, are poorly removed in conventional wastewater treatment plants (WWTPs). To reduce the release of these substances into the aquatic environment, advanced wastewater treatments are necessary. In this context, two large-scale pilot advanced treatments were tested in parallel over more than one year at the municipal WWTP of Lausanne, Switzerland. The treatments were: i) oxidation by ozone followed by sand filtration (SF) and ii) powdered activated carbon (PAC) adsorption followed by either ultrafiltration (UF) or sand filtration. More than 70 potentially problematic substances (pharmaceuticals, pesticides, endocrine disruptors, drugs metabolites and other common chemicals) were regularly measured at different stages of treatment. Additionally, several ecotoxicological tests such as the yeast estrogen screen, a combined algae bioassay and a fish early life stage test were performed to evaluate effluent toxicity. Both treatments significantly improved the effluent quality. Micropollutants were removed on average over 80% compared with raw wastewater, with an average ozone dose of 5.7 mg O 3 l -1 or a PAC dose between 10 and 20 mg l -1 . Depending on the chemical properties of the substances (presence of electron-rich moieties, charge and hydrophobicity), either ozone or PAC performed better. Both advanced treatments led to a clear reduction in toxicity of the effluents, with PAC-UF performing slightly better overall. As both treatments had, on average, relatively similar efficiency, further criteria relevant to their implementation were considered, including local constraints (e.g., safety, sludge disposal, disinfection), operational feasibility and cost. For sensitive receiving waters (drinking water resources or recreational waters), the PAC-UF treatment, despite its current higher cost, was considered to be the most suitable option, enabling good removal of most micropollutants and macropollutants without forming problematic by-products, the strongest decrease in toxicity and a total disinfection of the effluent.

show abstract

“…Transcripts chosen for analysis included those that code for protein targets of prochloraz as well as gene products that represent potential control points in the HPG axis (Ankley et al, 2005;Villeneuve et al, 2007b). Based on past studies with prochloraz, most of the gene expression work focused on females, as they appear to be more sensitive to endocrine-mediated effects of the fungicide than males (Ankley et al, 2005;Thorpe et al, 2007). However, we did measure transcripts of few genes in testis samples: cyp17, androgen receptor (ar), and a cytochrome P450 side-chain cleavage (cyp11a).…”

Section: Methodsmentioning

confidence: 99%

“…However, prochloraz can bind to and inhibit the activity of a wide variety of CYPs, including some involved in steroid synthesis in vertebrates. For example, studies have shown that the fungicide effectively inhibits aromatase (CYP19) activity in both fish and mammals, blocking the conversion of testosterone (T) to 17b-estradiol (E2) and acting in vivo as an antiestrogen (Ankley et al, 2005;Mason et al, 1987;Sanderson et al, 2002;Thorpe et al, 2007;Vinggaard et al, 2000). Prochloraz also can cause antiandrogenic effects in vertebrates either through direct antagonism of the androgen receptor or through inhibition of cytochrome P450 c17a-hydroxylase/17,20-lyase (CYP17), a steroidogenic enzyme involved in T production (Blystone et al, 2007;Noriega et al, 2005;Vinggaard et al, 2002Vinggaard et al, , 2005.…”

mentioning

confidence: 99%

Dynamic Nature of Alterations in the Endocrine System of Fathead Minnows Exposed to the Fungicide Prochloraz

Ankley

Bencic

Cavallin

et al. 2009

Toxicological Sciences

View full text Add to dashboard Cite

The vertebrate hypothalamic-pituitary-gonadal (HPG) axis is controlled through various feedback mechanisms that maintain a dynamic homeostasis in the face of changing environmental conditions, including exposure to chemicals. We assessed the effects of prochloraz on HPG axis function in adult fathead minnows (Pimephales promelas) at multiple sampling times during 8-day exposure and 8-day depuration/recovery phases. Consistent with one mechanism of action of prochloraz, inhibition of cytochrome P450 (CYP) 19 aromatase activity, the fungicide depressed ex vivo ovarian production and plasma concentrations of 17b-estradiol (E2) in female fish. At a prochloraz water concentration of 30 mg/l, inhibitory effects on E2 production were transitory and did not persist during the 8-day exposure phase. At 300 mg/l prochloraz, inhibition of E2 production was evident throughout the 8-day exposure but steroid titers recovered within 1 day of cessation of exposure. Compensation or recovery of steroid production in prochloraz-exposed females was accompanied by upregulation of several ovarian genes associated with steroidogenesis, including cyp19a1a, cyp17 (hydroxylase/lyase), cyp11a (cholesterol side-chain cleavage), and follicle-stimulating hormone receptor. In male fathead minnows, the 8-day prochloraz exposure decreased testosterone (T) production, possibly through inhibition of CYP17. However, as for E2 in females, ex vivo testicular production and plasma concentrations of T recovered within 1 day of stopping exposure. Steroidogenic genes upregulated in testis included cyp17 and cyp11a. These studies demonstrate the adaptability of the HPG axis to chemical stress and highlight the need to consider the dynamic nature of the system when developing approaches to assess potential risks of endocrine-active chemicals.

show abstract

Associations between altered vitellogenin concentrations and adverse health effects in fathead minnow (Pimephales promelas)

Cited by 71 publications

References 28 publications

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Estrogenic activity of tropical fish food can alter baseline vitellogenin concentrations in male fathead minnow (Pimephales promelas)

Treatment of micropollutants in municipal wastewater: Ozone or powdered activated carbon?

Dynamic Nature of Alterations in the Endocrine System of Fathead Minnows Exposed to the Fungicide Prochloraz

Contact Info

Product

Resources

About