Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta

Stejskalová, Karla; Jánová, Eva; Horecky, Cenek; Horecka, Eliska; Václavek, Petr; Hubálek, Zdeněk; Relling, K; Cvanová, Michaela; D’Amico, Gianluca; Mihalca, Andrei Daniel; Modrý, David; Knoll, A.; Hořín, Petr

doi:10.1007/s11033-019-04900-w

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…Three different equine breed groups were studied: the Camargue horses from the Rhone river Delta ( n = 35), Romanian horses from the Danube Delta ( n = 35) and local African horses from Cote d'Ivoire ( n = 24). The Camargue and the Romanian horses were characterized previously 29,30 . The African horses represent a random selection of local horses kept by individual owners in the area of Abidjan, Cote d'Ivoire.…”

Section: Methodsmentioning

confidence: 99%

“…The Camargue and the Romanian horses were characterized previously. 29,30 The African horses represent a random selection of local horses kept by individual owners in the area of Abidjan, Cote d'Ivoire. Peripheral blood samples were collected, based on the owners' consent, for diagnostic purposes in the region of Abidjan.…”

Section: Samplesmentioning

confidence: 99%

“…All peripheral blood samples were collected by licensed veterinarians according to best practices and in full compliance with all legal and ethical standards for the purposes of other research projects 29,30 and/or for diagnostic purposes, and shared. No additional approvals were thus necessary for this study.…”

Section: Samplesmentioning

confidence: 99%

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Newly identified variability of the antigen binding site coding sequences of the equine major histocompatibility complex class I and class II genes

Plášil

Oppelt

Klumplerová

et al. 2023

HLA

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The major histocompatibility complex (MHC) with its class I and II genes plays a crucial role in the immune response to pathogens by presenting oligopeptide antigens to various immune response effector cells. In order to counteract the vast variability of infectious agents, MHC class I and II genes usually retain high levels of SNPs mainly concentrated in the exons encoding the antigen binding sites. The aim of the study was to reveal new variability of selected MHC genes with a special focus on MHC class I physical haplotypes. Long‐range NGS to was used to identify exon 2–exon 3 alleles in three genetically distinct horse breeds. A total of 116 allelic variants were found in the MHC class I genes Eqca‐1, Eqca‐2, Eqca‐7 and Eqca‐Ψ, 112 of which were novel. The MHC class II DRA locus was confirmed to comprise five exon 2 alleles, and no new sequences were observed. Additional variability in terms of 15 novel exon 2 alleles was identified in the DQA1 locus. Extensive overall variability across the entire MHC region was confirmed by an analysis of MHC‐linked microsatellite loci. Both diversifying and purifying selection were detected within the MHC class I and II loci analyzed.

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Section: Methodsmentioning

confidence: 99%

Section: Samplesmentioning

confidence: 99%

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Newly identified variability of the antigen binding site coding sequences of the equine major histocompatibility complex class I and class II genes

Plášil

Oppelt

Klumplerová

et al. 2023

HLA

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“…In domestic animals, polymorphisms in various TLR genes have been associated with mastitis and other economically important traits in cattle (reviewed in Novák 2014 ). In horses, an association between a SNP in TLR4 and West Nile virus infection has been reported (Stejskalova et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Twelve toll-like receptor (TLR) genes in the family Equidae – comparative genomics, selection and evolution

Stejskalova,

Janova,

Splichalova

et al. 2023

Vet Res Commun

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Toll-like receptors (TLRs) represent an important part of the innate immune system. While human and murine TLRs have been intensively studied, little is known about TLRs in non-model species. The order Perissodactyla comprises a variety of free-living and domesticated species exposed to different pathogens in different habitats and is therefore suitable for analyzing the diversity and evolution of immunity-related genes. We analyzed TLR genes in the order Perissodactyla with a focus on the family Equidae. Twelve TLRs were identified by bioinformatic analyses of online genomic resources; their sequences were confirmed in equids by genomic DNA re-sequencing of a panel of nine species. The expression of TLR11 and TLR12 was confirmed in the domestic horse by cDNA sequencing. Phylogenetic reconstruction of the TLR gene family in Perissodactyla identified six sub-families. TLR4 clustered together with TLR5; the TLR1-6-10 subfamily showed a high degree of sequence identity. The average estimated evolutionary divergence of all twelve TLRs studied was 0.3% among the Equidae; the most divergent CDS were those of Equus caballus and Equus hemionus kulan (1.34%) in the TLR3, and Equus africanus somaliensis and Equus quagga antiquorum (2.1%) in the TLR1 protein. In each TLR gene, there were haplotypes shared between equid species, most extensively in TLR3 and TLR9 CDS, and TLR6 amino acid sequence. All twelve TLR genes were under strong negative overall selection. Signatures of diversifying selection in specific codon sites were detected in all TLRs except TLR8. Differences in the selection patterns between virus-sensing and non-viral TLRs were observed.

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