Associations of horse age, joint type, and osteochondral injury with serum and synovial fluid concentrations of type II collagen biomarkers in Thoroughbreds

Nicholson, Anne M.; Trumble, Troy N.; Merritt, K.A.; Brown, M. P.

doi:10.2460/ajvr.71.7.741

Cited by 23 publications

(28 citation statements)

References 36 publications

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“…22,23 It has been suggested that there may be a fundamental difference in skeletal metabolism of osteoarthritis patients, compared with healthy individuals, that could affect biomarker concentrations. 24 Although unproven in the present study, this may be similar in horses because the present study found decreased serum concentrations similar to some other equine biomarker studies 14,18,25,26 of naturally occurring joint disease.…”

Section: Discussionsupporting

confidence: 75%

“…It is not uncommon for younger horses to have higher biomarker concentrations than mature horses. 25 In the present study, no association between age and serum SDF-1 concentrations was found, but the association between age and biomarker concentrations may not be straightforward. 25 Ideally, osteoarthritis biomarkers should be investigated in multiple body fluids (serum, urine, and synovial fluid).…”

Section: Discussioncontrasting

confidence: 57%

“…25 In the present study, no association between age and serum SDF-1 concentrations was found, but the association between age and biomarker concentrations may not be straightforward. 25 Ideally, osteoarthritis biomarkers should be investigated in multiple body fluids (serum, urine, and synovial fluid). 20,21,27 Collection of urine and blood from patients is associated with less complications, compared with synovial fluid.…”

Section: Discussioncontrasting

confidence: 57%

“…Factors such as chronicity, exercise, and previous medical treatments were mostly unknown. Although this is not an uncommon problem when examining clinical cases of naturally occurring joint disease for biomarker studies, 14,18,25,26,[33][34][35][36][37][38][39] the potential effect on biomarker concentrations can be quite profound. For example, administration of phenylbutazone, 40 lidocaine, 41 methylprednisolone acetate, 42 and triamcinolone acetonide 43 results in changes in biomarker concentrations in equine joints.…”

Section: Discussionmentioning

confidence: 99%

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Concentrations of stromal cell-derived factor-1 in serum, plasma, and synovial fluid of horses with osteochondral injury

Dymock¹,

Brown²,

Merritt³

et al. 2014

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Results suggested that serum SDF-1 concentrations were more sensitive than plasma and synovial fluid concentrations for detection of osteochondral injury in the fetlock or carpal joint of racehorses. Analysis of serum and synovial SDF-1 concentrations in horses with experimentally induced joint injury may help define the onset and progression of post-traumatic osteoarthritis and aid in the evaluation of anti-inflammatory treatments.

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Section: Discussionsupporting

confidence: 75%

Section: Discussioncontrasting

confidence: 57%

Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Concentrations of stromal cell-derived factor-1 in serum, plasma, and synovial fluid of horses with osteochondral injury

Dymock¹,

Brown²,

Merritt³

et al. 2014

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“…This is the first study in which the long-term consequences of an impact joint injury have been evaluated in STBRs with spontaneous PTOA, regularly trained after a rehabilitation period, and using biomarkers to assess disease activity. Previous studies focused on OA biomarkers in experimental horse models spread their observations to a limited number of weeks following the acute joint injury [2, 7, 27, 38–40]. …”

Section: Discussionmentioning

confidence: 99%

Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career

et al. 2016

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BackgroundJoint impact injuries initiate a progressive articular damage finally leading to post-traumatic osteoarthritis (PTOA). Racehorses represent an ideal, naturally available, animal model of the disease. Standardbred racehorses developing traumatic osteoarthritis of the fetlock joint during the first year of their career were enrolled in our study. Age-matched controls were contemporarily included. Biomarker levels of equine osteoarthritis were measured in serum and synovial fluid (SF) at baseline, and repeated yearly over the next 4 years of training (from T1 to T4). The effect of time and disease on the biomarker concentrations were analysed, and their relationship with clinical and radiographic parameters were assessed. We hypothesized that the kinetics of pro-inflammatory cytokines and structural biomarkers of joint disease would demonstrate progression of degenerative joint status during post-traumatic osteoarthritis and clarify the effect of early joint trauma.ResultsThe concentrations of IL1-ß, IL-6, TNF-α in the SF of PTOA group peaked at T0, decreased at T1, and then progressively increased with time, reaching levels higher than those observed at baseline starting from T3. CTXII and COMP levels were similar in PTOA and control horses at baseline, and increased in serum and synovial fluid of PTOA horses starting from T2 (serum and synovial CTXII, and serum COMP) or T3 (synovial COMP). The percentual change of TNF-α in the SF of the affected joints independently contributed to explaining the radiological changes at T3 vs T2 and T4 vs T3.Conclusions Temporal changes of selected biomarkers in STBRs with an acute episode of traumatic fetlock OA demonstrated that long-term increased concentrations of inflammatory cytokines, type II collagen fragments and COMP, in the SF and serum, are related to PTOA. Based on the observed decrease in inflammatory merkers at T1, we hypothesize that the progression of PTOA could be effectively modulated by proper treatment strategies. Annual variations of synovial concentration of TNF-α can reliably predict radiographic progression of PTOA.

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Magnetic Capture of a Molecular Biomarker from Synovial Fluid in a Rat Model of Knee Osteoarthritis

et al. 2015

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Biomarker development for osteoarthritis (OA) often begins in rodent models, but can be limited by an inability to aspirate synovial fluid from a rodent stifle (similar to the human knee). To address this limitation, we have developed a magnetic nanoparticle-based technology to collect biomarkers from a rodent stifle, termed magnetic capture. Using a common OA biomarker - the c-terminus telopeptide of type II collagen (CTXII) - magnetic capture was optimized in vitro using bovine synovial fluid and then tested in a rat model of knee OA. Anti-CTXII antibodies were conjugated to the surface of superparamagnetic iron oxide-containing polymeric particles. Using these anti-CTXII particles, magnetic capture was able to estimate the level of CTXII in 25 µL aliquots of bovine synovial fluid; and under controlled conditions, this estimate was unaffected by synovial fluid viscosity. Following in vitro testing, anti-CTXII particles were tested in a rat monoiodoacetate model of knee OA. CTXII could be magnetically captured from a rodent stifle without the need to aspirate fluid and showed 10 fold changes in CTXII levels from OA-affected joints relative to contralateral control joints. Combined, these data demonstrate the ability and sensitivity of magnetic capture for post-mortem analysis of OA biomarkers in the rat.

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Associations of horse age, joint type, and osteochondral injury with serum and synovial fluid concentrations of type II collagen biomarkers in Thoroughbreds

Abstract: Horse age, osteochondral injury, and joint type all significantly affected type II collagen biomarker concentrations in SF and serum of Thoroughbreds.

Cited by 23 publications

References 36 publications

Concentrations of stromal cell-derived factor-1 in serum, plasma, and synovial fluid of horses with osteochondral injury

Concentrations of stromal cell-derived factor-1 in serum, plasma, and synovial fluid of horses with osteochondral injury

Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career

Magnetic Capture of a Molecular Biomarker from Synovial Fluid in a Rat Model of Knee Osteoarthritis

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