Associations of Pulmonary Fibrosis with Peripheral Blood Th1/Th2 Cell Imbalance and EBF3 Gene Methylation in Uygur Pigeon Breeder’s Lung Patients

Wu, Chao; Luo, Zujin; Pang, Bao-sen; Wang, Wenyi; Deng, Mingqin; Jin, Rong; Muhataer, Xirennayi; Li, Yafang; Li, Qifeng; Yang, Xiaohong

doi:10.1159/000490208

Cited by 13 publications

(18 citation statements)

References 20 publications

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“…As a mucolytic agent, NAC not only has the effect of directly dissolving mucus expectorant but also has the effect of anti-in ammatory, antioxidant, increasing the secretion and activity of active substances in the lung (12)(13)(14)(15). A large number of free radicals are produced in ARDS patients.…”

Section: Discussionmentioning

confidence: 99%

Experience of N-acetylcysteine airway management in the successful treatment of one case of critical condition with COVID-19

Liu¹,

Luo²,

Qian³

et al. 2020

Preprint

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Abstract Object: To report the successful diagnosis and treatment of a patient with critical condition of novel coronavirus pneumonia (COVID-19) and to summarize its clinical features and airway management experience in successful treatment.Methods: Retrospectively analyzed the successful management of one case of COVID-19 with critical condition combined respiratory failure and discussed the clinical characteristics and airway management of the patient in conjunction with a review of the latest literature.Results: A patient with an anastomotic fistula after radical treatment of esophageal cancer and right-side encapsulated pyopneumothorax was admitted with cough and dyspnea and was diagnosed with novel coronavirus pneumonia and malnutrition by pharyngeal swab nucleic acid test in combination with chest CT. The patient was treated with antibiotics, antiviral and antibacterial medications, respiratory support, expectorant nebulization, and nutritional support, expressed progressive deterioration. Endotracheal intubation and mechanical ventilation were performed since the onset of the type Ⅱ respiratory failure on the 13th day of admission. The patient had persistent refractory hypercapnia after mechanical ventilation. Based on the treatment mentioned above, combined with repeated bronchoalveolar lavage by using N-acetylcysteine (NAC) inhalation solution, the patient's refractory hypercapnia was gradually improved. It was cured and discharged after being given the mechanical ventilation for 26 days as well as 46 days of hospitalization, currently is surviving well.Conclusion: Patients with severe conditions of novel coronavirus pneumonia often encounter bacterial infection in their later illness-stages. They may suffer respiratory failure and refractory hypercapnia that is difficult to improve due to excessive mucus secretion leading to small airway obstruction. In addition to the use of reasonable antibiotics and symptomatic respiratory support and other treatment, timely artificial airway and repeated bronchoalveolar NAC inhalation solution lavage, expectorant and other airway management are essential for such patients.

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Section: Discussionmentioning

confidence: 99%

Experience of N-acetylcysteine airway management in the successful treatment of one case of critical condition with COVID-19

Liu¹,

Luo²,

Qian³

et al. 2020

Preprint

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“…Our ndings are consistent with Wu's study of INF-g dominance (Th1 cytokine) in pigeon breeder's lung patients at the acute/ sub-acute stage and IL-4 dominance (Th2 cytokine) at the chronic stage aer pulmonary brosis occurred. 48 Recent studies have found that DCs can regulate polarization of Th1/Th2 cells in a variety of diseases, but this regulation in silicosis is rarely reported.…”

Section: 36mentioning

confidence: 99%

Dendritic cells trigger imbalance of Th1/Th2 cells in silica dust exposure rat model via MHC-II, CD80, CD86 and IL-12

et al. 2018

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Silicosis is one of the most common occupational respiratory diseases caused by inhaling silica dust over a prolonged period of time, and the progression of silicosis is accompanied with chronic inflammation and progressive pulmonary fibrosis, in which dendritic cells (DCs), the most powerful antigen presentation cell (APC) in the immune response, play a crucial role. To investigate the role of DCs in the development of silicosis, we established an experimental silicosis rat model and examined the number of DCs and alveolar macrophages (AMs) in lung tissues using immunofluorescence over 84 days. Additionally, to obtain an overview of the immunological changes in rat lung tissues, a series of indicators including Th1/Th2 cells, IFN-g, IL-4, MHC-II, CD80/86 and IL-12 were detected using flow cytometry and an enzyme-linked immunosorbent assay (ELISA) as well as a real-time polymerase chain reaction (PCR) assay. We observed that the number of DCs slightly increased at the inflammatory stage, and it increased significantly at the final stage of fibrosis. Polarization of Th1 cells and IFN-g expressions were dominant during the inflammatory stage, whereas polarization of Th2 cells and IL-4 expressions were dominant during the fibrotic stage. The subsequent mechanistic study found that the expressions of MHC-II, CD80/86 and IL-12, which are the key molecules that connect DCs and Th cells, changed dynamically in the experimental silicosis rat model. The data obtained in this study indicated that the increase in DCs may contribute to polarization of Th1/Th2 cells via MHC-II, CD80/86, and IL-12 in silica dust-exposed rats.

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Section: Resultsmentioning

confidence: 99%

“…The existing relationships between the new candidate genes mentioned in this section and other human cancers except for the lung cancer, were also mentioned in the sixth column of Table 1. 32,51,[57][58][59][60][61][62][63][64] The main source we used for this part was DisGeNET database. 68 Comparison of some associated diseases with the new candidate genes can help in selecting them to be checked experimentally to prove their association with the NSCLC.…”

Section: Candidate New Genes In Association With Nsclcmentioning

confidence: 99%

Considering smoking status, coexpression network analysis of non–small cell lung cancer at different cancer stages, exhibits important genes and pathways

Mortezaei

Tavallaei

Hosseini

2019

J of Cellular Biochemistry

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Non–small cell lung cancer (NSCLC) is the most common subtype of lung cancer among smokers, nonsmokers, women, and young individuals. Tobacco smoking and different stages of the NSCLC have important roles in cancer evolution and require different treatments. Existence of poorly effective therapeutic options for the NSCLC brings special attention to targeted therapies by considering genetic alterations. In this study, we used RNA‐Seq data to compare expression levels of RefSeq genes and to find some genes with similar expression levels. We utilized the “Weighted Gene Co‐expression Network Analysis” method for three different datasets to create coexpressed genetic modules having relations with the smoking status and different stages of the NSCLC. Our results indicate seven important genetic modules having important associations with the smoking status and cancer stages. Based on investigated genetic modules and their biological explanation, we then identified 13 newly candidate genes and 7 novel transcription factors in association with the NSCLC, the smoking status, and cancer stages. We then examined those results using other datasets and explained our results biologically to illustrate some important genes in relation with the smoking status and metastatic stage of the NSCLC that can bring some crucial information about cancer evolution. Our genetic findings also can be used as some therapeutic targets for different clinical conditions of the NSCLC.

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Associations of Pulmonary Fibrosis with Peripheral Blood Th1/Th2 Cell Imbalance and EBF3 Gene Methylation in Uygur Pigeon Breeder’s Lung Patients

Cited by 13 publications

References 20 publications

Experience of N-acetylcysteine airway management in the successful treatment of one case of critical condition with COVID-19

Experience of N-acetylcysteine airway management in the successful treatment of one case of critical condition with COVID-19

Dendritic cells trigger imbalance of Th1/Th2 cells in silica dust exposure rat model via MHC-II, CD80, CD86 and IL-12

Considering smoking status, coexpression network analysis of non–small cell lung cancer at different cancer stages, exhibits important genes and pathways

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