Astaxanthin ameliorates cisplatin‐induced damage in normal human fibroblasts

Yamaguchi, Momoho; Tomihara, Kei; Heshiki, Wataru; Sakurai, Kotaro; Sekido, Katsuhisa; Tachinami, Hidetake; Moniruzzaman, Rohan; Inoue, Shuichiro; Fujiwara, Kumiko; Noguchi, Makoto

doi:10.1002/osi2.1031

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…Apoptotic cell death was assessed as previously described 23 . Human and mouse OSCC cell lines were cultured in the presence of PF-3758309 (2.5 μM) for 48 h. Apoptosis was assessed using a Fluorescein Isothiocyanate (FITC) Annexin V Apoptosis Detection Kit II (BD Pharmingen, San Diego, CA, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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PAK4 inhibition augments anti-tumour effect by immunomodulation in oral squamous cell carcinoma

Takatsuka,

Tachinami,

Suzuki

et al. 2024

Sci Rep

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Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumours, warranting novel treatments. Here, we examined the therapeutic efficacy of inhibiting p21 activated kinase 4 (PAK4) in OSCC and determined its immunomodulatory effect by focusing on the enhancement of anti-tumour effects. We examined PAK4 expression in OSCC cells and human clinical samples and analysed the proliferation and apoptosis of OSCC cells following PAK4 inhibition in vitro. We also investigated the effects of in vivo administration of a PAK4 inhibitor on immune cell distribution and T-cell immune responses in OSCC tumour-bearing mice. PAK4 was detected in all OSCC cells and OSCC tissue samples. PAK4 inhibitor reduced the proliferation of OSCC cells and induced apoptosis. PAK4 inhibitor significantly attenuated tumour growth in mouse and was associated with increased proportions of IFN-γ-producing CD8+ T-cells. Furthermore, PAK4 inhibitor increased the number of dendritic cells (DCs) and up-regulated the surface expression of various lymphocyte co-stimulatory molecules, including MHC-class I molecules, CD80, CD83, CD86, and CD40. These DCs augmented CD8+ T-cell activation upon co-culture. Our results suggest that PAK4 inhibition in OSCC can have direct anti-tumour and immunomodulatory effects, which might benefit the treatment of this malignancy.

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Section: Methodsmentioning

confidence: 99%

“…The proportion of apoptotic cells was calculated as the percentage of annexin V-positive cells. Annexin V-positive/PI-negative cells were considered early apoptotic cells, whereas annexin V-positive/PI-positive cells were considered late apoptotic cells 23 .…”

Section: Methodsmentioning

confidence: 99%

PAK4 inhibition augments anti-tumour effect by immunomodulation in oral squamous cell carcinoma

Takatsuka,

Tachinami,

Suzuki

et al. 2024

Sci Rep

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“…The five antioxidants inhibit transcription of pro‐inflammatory agents (eg, TNF‐α, IL‐1, IL‐6) via nuclear factor κβ (NF‐κβ) and exhibit well control of ROS on dysregulated inflammation of acute or chronic wounds 24,59 . Astaxanthin, EGCG, and curcumin inhibit NF‐κβ in the PDGF pathway in inflammatory cells improving chronic wound healing 60,61 . They are a promising treatment though at a specific concentration to enhance a cellular response 33 .…”

Section: Potential Interaction Of Growth Factors and Exogenous Antioxidants During Wound Healingmentioning

confidence: 99%

Rational selection of bioactive principles for wound healing applications: Growth factors and antioxidants

Viaña-Mendieta

Sánchez

Benavides

2021

International Wound Journal

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Wound healing is a complex process of communication between growth factors, reactive species of oxygen, cells, signalling pathways, and cytokines in the extracellular matrix, in which growth factors are the key regulators. In humans, the main regulators of the cellular responses in wound healing are five growth factors, namely EGF, bFGF, VEGF, and TGF‐β1. On the other hand, antioxidants such as astaxanthin, beta‐carotene, epigallocatechin gallate, delphinidin, and curcumin have been demonstrated to stimulate cell proliferation, migration and angiogenesis, and control inflammation, to suggest a practical approach to design new strategies to treat non‐healing cutaneous conditions. Based on the individual effects of growth factors and antioxidants, it may be envisioned that the use of both types of bioactives in wound healing formulations may have an additive or synergistic effect on the healing potential. This review addresses the effect of growth factors and antioxidants on wound healing‐related processes. Furthermore, a prospective on their potential additive or synergistic effect on wound healing formulations, based on their individual effects, is presented. This may serve as a guide for the development of a new generation of wound healing formulations.

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Oxidative Stress and Chemoradiation-Induced Oral Mucositis: A Scoping Review of In Vitro, In Vivo and Clinical Studies

Nguyen

Sangha

Pan

et al. 2022

IJMS

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Chemoradiation-induced mucositis is a debilitating condition of the gastrointestinal tract eventuating from antineoplastic treatment. It is believed to occur primarily due to oxidative stress mechanisms, which generate Reactive Oxygen Species (ROS). The aim of this scoping review was to assess the role of oxidative stress in the development of Oral Mucositis (OM). Studies from the literature, published in MEDLINE and SCOPUS, that evaluated the oxidative stress pathways or antioxidant interventions for OM, were retrieved to elucidate the current understanding of their relationship. Studies failing inclusion criteria were excluded, and those suitable underwent data extraction, using a predefined data extraction table. Eighty-nine articles fulfilled criteria, and these were sub-stratified into models of study (in vitro, in vivo, or clinical) for evaluation. Thirty-five clinical studies evaluated antioxidant interventions on OM’s severity, duration, and pain, amongst other attributes. A number of clinical studies sought to elucidate the protective or therapeutic effects of compounds that had been pre-determined to have antioxidant properties, without directly assessing oxidative stress parameters (these were deemed “indirect evidence”). Forty-seven in vivo studies assessed the capacity of various compounds to prevent OM. Findings were mostly consistent, reporting reduced OM severity associated with a reduction in ROS, malondialdehyde (MDA), myeloperoxidase (MPO), but higher glutathione (GSH) and superoxide dismutase (SOD) activity or expression. Twenty-one in vitro studies assessed potential OM therapeutic interventions. The majority demonstrated successful a reduction in ROS, and in select studies, secondary molecules were assessed to identify the mechanism. In summary, this review highlighted numerous oxidative stress pathways involved in OM pathogenesis, which may inform the development of novel therapeutic targets.

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Astaxanthin ameliorates cisplatin‐induced damage in normal human fibroblasts

Cited by 4 publications

References 29 publications

PAK4 inhibition augments anti-tumour effect by immunomodulation in oral squamous cell carcinoma

PAK4 inhibition augments anti-tumour effect by immunomodulation in oral squamous cell carcinoma

Rational selection of bioactive principles for wound healing applications: Growth factors and antioxidants

Oxidative Stress and Chemoradiation-Induced Oral Mucositis: A Scoping Review of In Vitro, In Vivo and Clinical Studies

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