Astragali Radix–Codonopsis Radix–Jujubae Fructus water extracts ameliorate exercise‐induced fatigue in mice via modulating gut microbiota and its metabolites

He, Pan; Chen, Lei; Qin, Xue; Du, Guanhua; Li, Zhenyu

doi:10.1002/jsfa.11866

Cited by 10 publications

(9 citation statements)

References 50 publications

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“…As bioactive molecules are involved in the recognition and signal transduction of proteins, glycerophospholipid is one of the phospholipids in the cell membrane. He and coworkers declared that combining Astragali Radix and Codonopsis Radix with Jujubae Fructus could protect cell membrane structure from oxidative damage and ameliorate fatigue by regulating glycerophospholipid metabolism in mice [ 55 ]. In amino acid metabolism, the metabolism of BCAAs increases in the MFGM group.…”

Section: Resultsmentioning

confidence: 99%

Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice

Zou

Yokoyama

Liu³

et al. 2023

Antioxidants

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Milk fat globule membranes (MFGMs) are complex structures that incorporate bioactive proteins and lipids to assist in infant development. However, the antifatigue and antioxidant potentials of MFGM have not been investigated. In this study, repeated force swimming measured fatigue in male BALB/c mice fed MFGM and saline for 18 weeks. The MFGM supplementation increased the time to exhaustion by 42.7% at 6 weeks and 30.6% at 14 weeks (p < 0.05). Fatigue and injury-related biomarkers, including blood glucose, lactic acid, and lactate dehydrogenase, were ameliorated after free swimming (p < 0.05). The activity of antioxidant enzymes in blood serum increased at 18 weeks, while malondialdehyde (MDA) content decreased by 45.0% after the MFGM supplementation (p < 0.05). The Pearson correlation analysis showed a high correlation between fatigue-related indices and antioxidant levels. The increased protein expression of hepatic Nrf2 reduced the protein expression of Caspase-3 in the gastrocnemius muscle (p < 0.05). Moreover, the MFGM supplementation increased the relative abundance of Bacteroides, Butyricimonas, and Anaerostipes. Our results demonstrate that MFGM may maintain redox homeostasis to relieve fatigue, suggesting the potential application of MFGM as an antifatigue and antioxidant dietary supplement.

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Section: Resultsmentioning

confidence: 99%

Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice

Zou

Yokoyama

Liu³

et al. 2023

Antioxidants

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“…The composition and diversity of the gut commensal flora exert pivotal roles in orchestrating gastrointestinal functions and gut immunity, and dysbiosis is considered a detrimental factor of UC. In the JW formula, GL [ 4 , 43 ], CWT [ 44 , 45 ], BD [ 46 ], CH [ 47 , 48 ], SK [ 49 , 50 ], and BT [ 51 , 52 ] could reshape the colonization of the gut microorganisms by their prebiotic-like activities. In full accordance with our results, DSS-induced colitis decreases the richness and diversity of the gut microbiota [ 53 ], both of which were reversed by the JW formula, highlighting its contribution to the remodeling of gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula

Yan

Tang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Inflammatory bowel disease (IBD) is a major cause of morbidity and mortality due to its repetitive remission and relapse. The Jian-Wei-Yu-Yang (JW) formula has a historical application in the clinic to combat gastrointestinal disorders. The investigation aimed to explore the molecular and cellular mechanisms of JW. Methods. 2% dextran sodium sulfate (DSS) was diluted in drinking water and given to mice for 5 days to establish murine models of experimental colitis, and different doses of JW solution were administered for 14 days. Network pharmacology analysis and weighted gene co-expression network analysis (WGCNA) were utilized to predict the therapeutic role of JW against experimental colitis and colitis-associated colorectal cancer (CAC). 16S rRNA sequencing and untargeted metabolomics were conducted using murine feces. Western blotting, immunocytochemistry, and wound healing experiments were performed to confirm the molecular mechanisms. Results. (1) Liquid chromatography with mass spectrometry was utilized to confirm the validity of the JW formula. The high dose of JW treatment markedly attenuated DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis. (2) The JW targets were related to the survival probability in patients with colorectal cancer, underlying a potential therapeutic value in CRC intervention. (3) Moreover, the JW therapy successfully rescued the decreased richness and diversity of microbiota, suppressed the potentially pathogenic phenotype of the gut microorganisms, and increased cytochrome P450 activity in murine colitis models. (4) Our in vitro experiments confirmed that the JW treatment suppressed caspase3-dependent pyroptosis, hypoxia-inducible factor 1α (HIF1α), and interleukin-1b (IL-1b) in the colon; facilitated the alternative activation of macrophages (Mφs); and inhibited tumor necrosis factor-α (TNFα)-induced reactive oxygen species (ROS) level in intestinal organoids (IOs). Conclusion. The JW capsule attenuated the progression of murine colitis by a prompt resolution of inflammation and bloody stool and by re-establishing a microbiome profile that favors re-epithelization and prevents carcinogenesis.

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“…The muscle exercise endurance of mice was measured by WEST according to the published protocol [ 18 ]. In short, the mice were individually loaded with a lead (7% of body weight) on their tail root and placed in the swimming pool (diameter 30 cm × height 50 cm) filled with 30 cm deep water (maintained at 25 ± 1°C).…”

Section: Methodsmentioning

confidence: 99%

Deciphering the Molecular Mechanism of Yifei-Sanjie Pill in Cancer-Related Fatigue

Zhou

et al. 2023

Journal of Oncology

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Background. The incidence of cancer-related fatigue (CRF) is increasing, but its lack of clear pathogenesis makes its prevention and treatment difficult. Therefore, it is of great significance to clarify the pathogenesis of CRF and find effective methods to treat it. Methods. The CRF model was established by intraperitoneal injection of LLC cells in ICR mice to explore the pathogenesis of CRF and verify the therapeutic effect of the Yifei-Sanjie pill (YFSJ). The active components of YFSJ were found by LC/MS, the in vitro inflammatory infiltration model of skeletal muscle was constructed by TNF-α and C2C12 myoblasts, and the results of in vivo experiments were verified by this model. Results. Behavioral analysis results showed that YFSJ alleviated CRF; histological examination results showed that YFSJ could reverse the tumor microenvironment leading to skeletal muscle injury; ELISA and RNA-seq results showed that the occurrence of CRF and the therapeutic effect of YFSJ were closely related to the tumor inflammatory microenvironment; IHC and WB results showed that the occurrence of CRF and the therapeutic effect of YFSJ were closely related to the Stat3-related signaling pathway and autophagy. Conclusions. YFSJ can reduce the level of inflammation in the tumor microenvironment in vivo, inhibit the abnormal activation of the Stat3/HIF-1α/BNIP3 signaling pathway induced by tumor-related inflammation, thereby inhibiting the overactivation of mitophagy in skeletal muscle, and finally alleviate CRF. Quercetin, one of the components of YFSJ, plays an important role in inhibiting the phosphorylation activation of Stat3.

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Astragali Radix–Codonopsis Radix–Jujubae Fructus water extracts ameliorate exercise‐induced fatigue in mice via modulating gut microbiota and its metabolites

Cited by 10 publications

References 50 publications

Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice

Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice

Validation of the Anticolitis Efficacy of the Jian-Wei-Yu-Yang Formula

Deciphering the Molecular Mechanism of Yifei-Sanjie Pill in Cancer-Related Fatigue

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