Astragaloside IV ameliorates radiation-induced senescence via antioxidative mechanism

Abstract

Objectives Ageing is a universal and gradual process of organ deterioration. Radiation induces oxidative stress in cells, which leads to genetic damage and affects cell growth, differentiation and senescence. Astragaloside (AS)-IV has antioxidative, anti-apoptotic and anti-inflammatory properties. Methods To study the protective mechanism of AS-IV on radiation-induced brain cell senescence, we constructed a radiation-induced brain cell ageing model, using biochemical indicators, senescence-associated galactosidase (SA-β-gal) senescence staining, flow cytometry and Western blotting to analyse the AS-IV resistance mechanism to radiation-induced brain cell senescence. Key findings Radiation reduced superoxide dismutase (SOD) activity and expressions of cyclin-dependent kinase (CDK2), CDK4, cyclin E and transcription factor E2F1 proteins, and increased expressions of p21, p16, cyclin D and retinoblastoma (RB) proteins, malondialdehyde (MDA) activity, SA-β-gal–positive cells and cells stagnating in G1 phase. After treatment with AS-IV, the level of oxidative stress in cells significantly decreased and expression of proteins related to the cell cycle and ageing significantly changed. In addition, SA-β-gal–positive cells and cells arrested in G1 phase were significantly reduced. Conclusions These data suggest that AS-IV can antagonize radiation-induced brain cells senescence; and its mechanism may be related to p53-p21 and p16-RB signalling pathways of ageing regulation.

Guiqi Baizhu Decoction Alleviates Radiation Inflammation in Rats by Modulating the Composition of the Gut Microbiota

“…Many effective components of GQBZD have been proved to have antiradiation effect. Li et al demonstrated that Astragaloside IV showed radio protective effects on the hematopoietic system in mice [ 27 ], and Liu et al suggest that Astragaloside IV can antagonize radiation-induced brain cells senescence, and its mechanism may be related to p53-p21 and p16-RB signaling pathways of aging regulation [ 28 ]. Yue et al found that Paeoniflorin enhances its sensitivity to radiotherapy by regulating MLH1 and MSH1 protein to enhance the DNA damage and repair ability of melanoma cells [ 29 ].…”

Research progress on antiviral constituents in traditional Chinese medicines and their mechanisms of action

“…Agstragaloside IV, a major active compound extracted from the root of Huangqi, has extensive pharmacological activities, including anti-inflammatory, antioxidative, antiviral and anti-cancer functions (Liu, Shang, et al. 2020 ; Ge and He 2020 ; Hu et al. 2021 ).…”

Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage

“…Ionizing radiation- (IR-) induced DNA damage, senescence-associated inflammatory factors, and ROS are also hallmarks of cardiomyocyte senescence [ 21 ]. Senescence and the DNA damage-associated >BRCA1 and p53/p21 signaling axis regulated by IR can cause cardiovascular injury, which eventually leads to RIHD [ 22 , 23 ]. Cardiac cellular senescence can also manifest as inflammation- or ROS-induced telomere shortening and senescence-associated secretory phenotypes (SASPs) [ 16 , 24 ].…”