Astragaloside IV Inhibits the Progression of Non-Small Cell Lung Cancer Through the Akt/GSK-3β/β-Catenin Pathway

Abstract

Astragaloside IV (AS-IV) is an active ingredient in Astragalus membranaceus, and involved in various biological processes, such as regulating the immune system, and counteracting inflammation and malignancy. The aim of this study was to explore the effect of AS-IV on non-small cell lung cancer (NSCLC) cells. A cell counting kit (CCK)-8 assay and flow cytometry were performed to investigate cell survival and cell death, and Western blotting was performed to assess protein expression. We found that AS-IV inhibited the migration and proliferation of NSCLC cells, and caused a noticeable increase in cell death. Furthermore, the expression of Bax, a marker of cell death, was increased, while the expression of Bcl-2, an anti-apoptotic protein, was reduced. AS-IV also promoted cleavage of caspase-3, another indication of apoptosis. Finally, the Akt/GSK-3β/β-catenin axis was suppressed in response to AS-IV. Taken together, these findings provide evidence that AS-IV inhibits NSCLC development via inhibition of the Akt/GSK-3β/β-catenin signaling axis. We therefore propose that AS-IV represents a promising novel agent for the treatment of NSCLC.

Astragaloside IV (AS-IV) alleviates the malignant biological behavior of hepatocellular carcinomaviaWnt/β-catenin signaling pathway

“…8 Astragaloside IV (AS-IV) is a natural nonsynthetic active component puried from Astragalus membranaceus, which has been widespreadly used in conventional Chinese medicine to therapy diseases. In addition, previous studies have also demonstrated that AS-IV possess phylactic effects against angiocardiopathy, 9 liver brosis, 10 lung cancer, 11 breast cancer, 12 allergic inammation, 13 etc. Furthermore, the pharmacological mechanism also mainly included antioxidative, anti-brotic, anti-inammatory, anti-diabetes and immune regulation.…”

Antagonistic effects of IL-17 and Astragaloside IV on cortical neurogenesis and cognitive behavior after stroke in adult mice through Akt/GSK-3β pathway

“…Astragaloside IV (As-IV), a primary bioactive compound of Radix Astragali, Astragalus mongholicus Bunge (Fabaceae), has been described as potential neuroprotective effect in experimental models of Alzheimer's disease and cerebral ischemia 22 . It has been found As-IV protected neurons from apoptosis and autophagy 23,24 , some by regulating Akt/GSK-3β/ β-catenin pathway [25][26][27] . As-IV could be a new therapeutic drug candidate for post-stroke treatment and effectively promote neural stem cells (NSCs) proliferation and neurogenesis in transient cerebral ischemic brains 28 .…”

A Chinese Herbal Formula Suppresses Colorectal Cancer Migration and Vasculogenic Mimicry Through ROS/HIF-1α/MMP2 Pathway in Hypoxic Microenvironment

“…In this study, we examined whether AAM could inhibit hypoxia-induced VM formation and explored relative mechanisms. As reported above, AAM is composed of six kinds of Chinese herbs, including Astragali radix (AR), Curcumae radix Atractylodis macrocephalae rhizoma (AMR), Actinidia arguta (AA), Curcumae radix (CR), Benincasae semen (BS), and Ficus pumila linn (FPL), and a series of studies have reported that some of the compounds and active ingredients of AAM could suppress tumor growth (Lim et al, 2016;Bai et al, 2018;Jia et al, 2018;Zheng et al, 2019). Notably, in these compounds, chlorogenic acid is an antioxidant-rich compound, which could not only effectively scavenge free radicals, but also inhibit cancer-causing pathways (Kavi Rajan and Hussein, 2019).…”

“…Many of these herbs have antitumor and antioxidant effect. Astragaloside IV (Astragalus membranaceus extract) suppresses tumor progression through inhibiting Akt/GSK-3b/b-catenin signaling axis and enhances chemosensitivity via targeting caveolin-1 (CAV-1), which led to oxidative stress (Jia et al, 2018;Zheng et al, 2018). Actinidia arguta exerts antitumor effect by inhibiting tumor cell proliferation (Lim et al, 2016), Additionally, oleanolic acid (Actinidia arguta (Siebold & Zucc.)…”