Asymbiotic seed germination and in vitro seedling development of Acianthera prolifera (Orchidaceae)

Koene, Franciele Marx; Amano, Érika; Ribas, Luciana Lopes Fortes

doi:10.1016/j.sajb.2018.07.019

Cited by 20 publications

(26 citation statements)

References 26 publications

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“…A. ochreata and A. prolifera elongated and developed roots in WPM medium, supplemented with 1 g L -1 activated charcoal (Fig 4D), as recommended by Koene et al [13] in a previous study with A. prolifera. Finally, A. ochreata and A. prolifera achieved high survival rates [80%] after 12 months, when using commercial Forth1 substrates, composed of a mixture of coconut fiber, Pinus bark, and charcoal with fine vermiculite Eucatex1 (1:1).…”

Section: Plos Onementioning

confidence: 63%

“…The number of testa cells along the longitudinal axis of the seed was low (2-6) in D. liliputiana (2), D. zebrina (2), S. grandiflora (2), A. sonderiana (3), A. obovata (4), P. fusca (4), O. gracilis (5), and A. hatschbachii (6). On the other hand, there was a high number (9)(10)(11)(12)(13)(14) of testa cells in A. aphthosa (9), A. ochreata (10), and A. prolifera (14). According to Barthlott et al [25], the number of testa cells is coupled to cell division [38], a pattern that probably arises from slow or interrupted division in the integuments following fertilization.…”

Section: Plos Onementioning

confidence: 99%

“…Finally, A. ochreata and A. prolifera achieved high survival rates [80%] after 12 months, when using commercial Forth1 substrates, composed of a mixture of coconut fiber, Pinus bark, and charcoal with fine vermiculite Eucatex1 (1:1). Koene et al [13] had also successfully acclimatized A. prolifera (95% survival, after three months) using the same substrate and conditions of greenhouse. Therefore, our results offer potential possibilities for reintroduction programs in the future that can play a key role in reducing the threat of extinction for species of micro-orchids.…”

Section: Plos Onementioning

confidence: 99%

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Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

et al. 2020

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The morphological and morphometric characters of seeds belonging to 11 species of the subtribe Pleurothallidinae using light and scanning electron microscopy were studied to understand the in vitro germination process. Qualitative data (color, shape, ornamentation) and quantitative ones were also evaluated in seeds and embryos (length, width, volume and air space percentage between the integument and the embryo). The viability of the seeds was evaluated by in vitro germination in woody plant medium (WPM), and by analysis of the developmental stages of protocorms until seedling formation (two to 24 weeks). Morphometric data showed variations within the genus Acianthera and between species of different genera. The best germination and protocorm formation responses occurred with Acianthera prolifera (92%) and Acianthera ochreata (86%), with the formation of seedlings after 12 and 16 weeks of sowing, respectively. The seeds and embryos of A. prolifera and A. ochreata were larger (length, width, and volume) with a structural polarity that may have facilitated their germination comparing to others studied species. Other characteristics of A. prolifera seeds that may have contributed to these results include the presence of a thin testa without ornamentation and a suspensor. The protocorms of Anathalis obovata, Dryadella liliputiana, and Octomeria gracillis developed slowly in the WPM, not reaching the seedling stage in 24 weeks of cultivation. This morphological and morphometric study contributes to the understanding of asymbiotic germination of some micro-orchid species.

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Section: Plos Onementioning

confidence: 63%

Section: Plos Onementioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

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Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

et al. 2020

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“…It can adsorb harmful substances present in culture media, including impurities in agar, 5-hydroxymethylfurfural produced by sucrose during high-pressure sterilization, and phenoquinones secreted by explants during culture, as well as beneficial substances available in culture media, such as growth regulators, vitamin B6, folic acid, and nicotinic acid [2]. There are many reports on its effects such as anti-browning, improvement of primary culture survival rates, promotion of bud proliferation and seedling growth in the dark, and promotion of rooting [3][4][5][6][7]. However, the mechanism of action of AC in promoting plant growth has been rarely reported.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

Dong

Wang

et al. 2020

BMC Genet

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Background: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth. Results: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7373 in the leaves. In seedlings grown in the presence of AC, 9460 DEGs were upregulated and 7483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL, the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ, and PR1, and downregulated genes related to plant growth inhibition, including BKI1, ARR-B, DELLA, and ABF. Conclusions: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance.

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“…It can adsorb harmful substances present in culture media, including impurities in agar, 5hydroxymethylfurfural produced by sucrose during high-pressure sterilization, and phenoquinones secreted by explants during culture, as well as bene cial substances available in culture media, such as growth regulators, vitamin B6, folic acid, and nicotinic acid [2]. There are many reports on its effects such as anti-browning, improvement of primary culture survival rates, promotion of bud proliferation and seedling growth in the dark, and promotion of rooting [3][4][5][6][7]. However, the mechanism of action of AC in promoting plant growth has been rarely reported.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

Dong

Wang

et al. 2020

Preprint

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Background: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth. Results: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7,373 in the leaves. In seedlings grown in the presence of AC, 9,460 DEGs were upregulated and 7,483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL , the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ , and PR1 , and downregulated genes related to plant growth inhibition, including BKI1, ARR-B , DELLA , and ABF . Conclusions: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance.

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Asymbiotic seed germination and in vitro seedling development of Acianthera prolifera (Orchidaceae)

Cited by 20 publications

References 26 publications

Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

Asymbiotic germination and morphological studies of seeds of Atlantic Rainforest micro-orchids (Pleurothallidinae)

Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

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