Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development

Jiang, Xinlong; An, Wenzhong; Yang, Xiao Hong; Lin, Jie-ye; Ma, Shumei; Wang, Dajia

doi:10.1016/j.gep.2017.11.002

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…By 60 hours, cells displayed an up-regulation in either E3.5 TE or E3.5 ICM scores, suggesting that reprogramming towards a state resembling an earlier developmental stage relative to E4.5 EPI-like starting ESCs is occurring ( Figure 3A and 3C) . Reprogramming towards E3.5 ICM and to E3.5 TE-like state in 60 h (pulse I) cells was confirmed by the activation of Calcoco2 (Spangler et al, 2018) , Khdc3 (Boroviak et al, 2018) , and Bhmt (Lee et al, 2012; McClatchie et al, 2017), as well as by Crb3 (Jiang et al, 2018), Cldn7 (Moriwaki et al, 2007) and Cdx2 (Suzuki et al, 2022b) respectively ( Figure 3C ). In 96 h (pulse II) cells, clear lineage segregation into E4.5 blastocyst stage fates is observed ( Figure 3A and 3D ).…”

Section: Resultsmentioning

confidence: 87%

Chemically induced cell plasticity enables the generation of high-fidelity embryo model

Li,

Huang,

Guan

et al. 2024

Preprint

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SummaryEmbryo models open exciting opportunities for improving our understanding of development and advancing medicine. However, current models depend on intricate procedures and the integration of separately generated cell types. Here, we aimed to establish a chemical-only strategy to induce cellular plasticity and generate embryo founder-like cells. Within 60 hours, chemical-induced pluripotent stem cells activated early lineage specifiers,Cdx2andGata6, while maintainingOct4/ Pou5f1expression. Following a second 36-hour chemical treatment, founder-like cells developed into all blastocyst fates and self-assembled into embryo-like structures. These progressed into high-fidelity late-streak gastrulation stages within 4.5 days, displaying primitive streak formation via epithelial-to-mesenchymal transition, along with germ-layer tissues, amnion, and ectoplacental cone. Fate trajectories and molecular signatures from founder-like cells to chemically-induced embryo model (ci-MO) were defined, revealing remarkable similarity up to Embryonic day (E) 7.5 embryo. Summarily, ci-MO offers a straightforward, rapid, small-molecule-only, and high-fidelity approach to study embryonic development and cell fate specification.HighlightsGeneration of a direct, efficient, and sophisticated chemically induced embryo model (ci-MO)Two-pulse chemical induction reprograms ESCs into founder-like cells capable of developing all embryo fatesGastrulating ci-MOs display high morphological and molecular accuracyci-MOs reconstruct embryo developmental trajectories

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Section: Resultsmentioning

confidence: 87%

Chemically induced cell plasticity enables the generation of high-fidelity embryo model

Li,

Huang,

Guan

et al. 2024

Preprint

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“… 34 This suggests that PATJ plays a key role in angiogenesis by establishing and maintaining cell polarity. 35 …”

Section: Underlying Mechanisms Of Association Between Patj and Stroke Outcomesmentioning

confidence: 99%

Research Progress on PATJ and Underlying Mechanisms Associated with Functional Outcomes After Stroke

Wang

Lyu

2021

NDT

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Cell polarity is an intrinsic property of epithelial cells regulated by scaffold proteins. The CRB (crumbs) complex is known to play a predominant role in the dynamic cooperative network of polarity scaffold proteins. PATJ (PALS1-associated tight junction) is the core component in the CRB complex and has been highly conserved throughout evolution. PATJ is crucial to several important events in organisms’ survival, including embryonic development, cell polarity, and barrier establishment. A recent study shows that PATJ plays an important role in functional outcomes of stroke. In this article, we elaborate on the biological structure and physiological functions of PATJ and explore the underlying mechanisms of PATJ genetic polymorphism that are associated with poor functional outcomes in ischemic stroke.

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Noninvasive autologous mitochondria transport improves the quality and developmental potential of oocytes from aged mice

Yang

Wang

et al. 2022

F&S Science

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Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development

Cited by 4 publications

References 26 publications

Chemically induced cell plasticity enables the generation of high-fidelity embryo model

Chemically induced cell plasticity enables the generation of high-fidelity embryo model

Research Progress on PATJ and Underlying Mechanisms Associated with Functional Outcomes After Stroke

Noninvasive autologous mitochondria transport improves the quality and developmental potential of oocytes from aged mice

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