Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons

Zhang, Ye; Tsang, Tin Ki; Bushong, Eric A.; Chu, Li‐An; Chiang, Ann‐Shyn; Ellisman, Mark H.; Reingruber, Jürgen; Su, Chih‐Ying

doi:10.1101/427252

Cited by 23 publications

(45 citation statements)

References 60 publications

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“…First, we show that roughly one third of responding GSNs are activated by more than one taste modality, while conversely, single tastants generally elicit responses in several neurons. Interestingly, we detected neuronal deactivation responses, distinct from canonical calcium imaging signals, which occurred within concomitant firing in adjacent neurons, as described for electrical coupling in the adult fly olfactory system (51,52). Using DisCo and immunostainings, we identified distinct and common molecular markers in TOG/DOG neurons.…”

Section: Introductionmentioning

confidence: 62%

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Maier

Biočanin

Bues

et al. 2020

Preprint

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The ability to evaluate food palatability is innate in all animals, ensuring their survival. The external taste organ in Drosophila larvae is composed of only few sensory neurons but enables discrimination between a wide range of chemicals and displays high complexity in receptor gene expression and physiological response profile. It remains largely unknown how the discrepancy between a small neuronal number and the perception of a large sensory space is genetically and physiologically resolved. We tackled dissection of taste sensory coding at organ level with cellular resolution in the fruit fly larva by combining whole-organ calcium imaging and single-cell transcriptomics to map physiological properties and molecular features of individual neurons. About one third of gustatory sense neurons responded to multiple tastants, showing a rather large degree of multimodality within the taste organ. Further supporting the notion of signal integration at the periphery, we observed neuronal deactivation events within simultaneous neighboring responses, suggesting inter-cellular communication through electrical coupling and thus providing an additional level in how neurons may encode taste sensing. Interestingly, we identified neurons responding to both mechanical and taste stimulation, indicating potential multisensory integration. On a molecular level, chemosensory cells show heterogeneity in neuromodulator expression. In addition to a broad cholinergic profile, markers on dopaminergic, glutamatergic or neuropeptidergic pathways are present either in distinct cell populations or are seemingly co-expressed. Our data further extend the sensory capacity of the larval taste system pointing towards an unanticipated degree of multimodal and multisensory coding principles.

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Section: Introductionmentioning

confidence: 62%

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Maier

Biočanin

Bues

et al. 2020

Preprint

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“…10: 200252 heavy metal staining (figure 1c) [20]. CryoChem has been used in D. melanogaster to create three-dimensional reconstructions of several distinct, genetically marked OSNs in different sensilla by serial block-face scanning EM [20,46]. These observations provide useful insights into the relationship between OSN anatomy and olfactory physiology, as discussed below.…”

Section: The Morphology and Cell Biology Of Olfactory Sensillamentioning

confidence: 99%

Molecular mechanisms of olfactory detection in insects: beyond receptors

Schmidt

Benton

2020

Open Biol.

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Insects thrive in diverse ecological niches in large part because of their highly sophisticated olfactory systems. Over the last two decades, a major focus in the study of insect olfaction has been on the role of olfactory receptors in mediating neuronal responses to environmental chemicals. In vivo , these receptors operate in specialized structures, called sensilla, which comprise neurons and non-neuronal support cells, extracellular lymph fluid and a precisely shaped cuticle. While sensilla are inherent to odour sensing in insects, we are only just beginning to understand their construction and function. Here, we review recent work that illuminates how odour-evoked neuronal activity is impacted by sensillar morphology, lymph fluid biochemistry, accessory signalling molecules in neurons and the physiological crosstalk between sensillar cells. These advances reveal multi-layered molecular and cellular mechanisms that determine the selectivity, sensitivity and dynamic modulation of odour-evoked responses in insects.

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“…Specific effects, for example the non-synaptic interaction between ORNs (Su et al, 2012; Zhang et al, 2019).…”

Section: The Spatio-temporal Structure Of Odor Stimuli In a Natural Ementioning

confidence: 99%

Odor Stimuli: Not Just Chemical Identity

Pannunzi

Nowotny

2019

Front. Physiol.

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In most sensory modalities the underlying physical phenomena are well understood, and stimulus properties can be precisely controlled. In olfaction, the situation is different. The presence of specific chemical compounds in the air (or water) is the root cause for perceived odors, but it remains unknown what organizing principles, equivalent to wavelength for light, determine the dimensions of odor space. Equally important, but less in the spotlight, odor stimuli are also complex with respect to their physical properties, including concentration and time-varying spatio-temporal distribution. We still lack a complete understanding or control over these properties, in either experiments or theory. In this review, we will concentrate on two important aspects of the physical properties of odor stimuli beyond the chemical identity of the odorants: (1) The amplitude of odor stimuli and their temporal dynamics. (2) The spatio-temporal structure of odor plumes in a natural environment. Concerning these issues, we ask the following questions: (1) Given any particular experimental protocol for odor stimulation, do we have a realistic estimate of the odorant concentration in the air, and at the olfactory receptor neurons? Can we control, or at least know, the dynamics of odorant concentration at olfactory receptor neurons? (2) What do we know of the spatio-temporal structure of odor stimuli in a natural environment both from a theoretical and experimental perspective? And how does this change if we consider mixtures of odorants? For both topics, we will briefly summarize the underlying principles of physics and review the experimental and theoretical Neuroscience literature, focusing on the aspects that are relevant to animals’ physiology and behavior. We hope that by bringing the physical principles behind odor plume landscapes to the fore we can contribute to promoting a new generation of experiments and models.

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Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons

Cited by 23 publications

References 60 publications

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Multimodal and multisensory coding in theDrosophilalarval peripheral gustatory center

Molecular mechanisms of olfactory detection in insects: beyond receptors

Odor Stimuli: Not Just Chemical Identity

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