2020
DOI: 10.1016/j.ab.2019.113517
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Asymmetric mutant-enriched polymerase chain reaction and quantitative DNA melting analysis of KRAS mutation in colorectal cancer

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Cited by 14 publications
(6 citation statements)
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“…Moreover, the test involves many washing and incubation steps with a total analysis time of about 2 h [ 48 ]. Compared to the previously reported biosensors for ctDNA detection in real samples ( Table S3 ) [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ], the advantages of the proposed multi-plex test are related mostly to the cost, run time, and simplicity, as well as the universality and advanced multiplexing potential.…”
Section: Discussionmentioning
confidence: 92%
“…Moreover, the test involves many washing and incubation steps with a total analysis time of about 2 h [ 48 ]. Compared to the previously reported biosensors for ctDNA detection in real samples ( Table S3 ) [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ], the advantages of the proposed multi-plex test are related mostly to the cost, run time, and simplicity, as well as the universality and advanced multiplexing potential.…”
Section: Discussionmentioning
confidence: 92%
“…Experimental and clinical studies typically require evaluation of the copy number of a target gene and the presence of polymorphisms, particularly mutations. Quantitative real time PCR with TaqMan probes is often used to evaluate copy number, and post-PCR DMA to detect polymorphisms 8,9,18 . A disadvantage of this approach is that the quantitative real time PCR is symmetric, while DMA with TaqMan probes requires an asymmetric PCR; therefore, two independent specific reactions are necessary.…”
Section: Resultsmentioning
confidence: 99%
“…5). www.nature.com/scientificreports www.nature.com/scientificreports/ The DMA assay permits detection of ~5% of mutant alleles in wild-type DNA 9 . A range of techniques are used for mutant enrichment during PCR.…”
Section: Products Of Pcr Amplification the Comparison Of Possible Stmentioning
confidence: 99%
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