Asymmetrical Carbonyl Stretching Bands in the Infrared Spectra of Esters.

Laato, Heimo; Isotalo, Reino; Cyvin, Bjørg N.; Brünvoll, J.; Hägen, G.

doi:10.3891/acta.chem.scand.21-2119

Cited by 14 publications

(7 citation statements)

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“…Local application of an ointment containing EGF to rabbit ear wounds similarly increased the thickness and cellularity of epithelium, inhibited wound contracture, and enhanced connective-tissue maturation (Franklin & Lynch, 1979). In our previous studies (Laato, 1986;Laato et al, 1986a, b), daily local application of 5 ,tg of EGF resulted in an augmented cellular response to injury, and biochemical analyses revealed dose-dependent increases in the accumulation of DNA, RNA, glycosaminoglycans and proteins, including collagen hydroxyproline (Laato et al, 1986a,b). Furthermore, the daily EGF treatment increased the local blood flow of granulation tissue above the control value (Laato, 1986).…”

Section: Discussionmentioning

confidence: 77%

“…The stimulatory role of EGF on granulation-tissue formation has been well established (Buckley et al, 1985;Carpenter & Zendegui, 1986;Laato, 1986;Laato et al, 1986a, b). In microscopic examination, sponges containing slow-release pellets of EGF stimulated tissue ingrowth and resulted in an enhanced organization of granulation tissue, and in greater angiogenesis than in the placebo-treated controls (Buckley et al, 1985).…”

Section: Discussionmentioning

confidence: 95%

“…In our previous studies (Laato, 1986;Laato et al, 1986a, b), daily local application of 5 ,tg of EGF resulted in an augmented cellular response to injury, and biochemical analyses revealed dose-dependent increases in the accumulation of DNA, RNA, glycosaminoglycans and proteins, including collagen hydroxyproline (Laato et al, 1986a,b). Furthermore, the daily EGF treatment increased the local blood flow of granulation tissue above the control value (Laato, 1986). Most of these observations can be explained by the mitogenic effect of EGF towards fibroblasts.…”

Section: Discussionmentioning

confidence: 96%

“…Epidermal growth factor (EGF) is considered to play a major role in induction of wound healing, along with the platelet-derived growth factor (PDGF) and the transforming growth factors a and , (TGF-a and TGF-/3) ). Treatment of experimental granulation tissue with EGF has been shown to result in increased contents of DNA, RNA and protein, particularly collagen, and of glycosaminoglycans (Niall et al, 1982;Buckley et al, 1985;Laato, 1986;Laato et al, 1986a,b). The fact that the collagen content of experimental granulomas is increased dramatically after daily application or sustained release of EGF for 7 days (Laato et al, 1986a;Buckley et al, 1985) suggests that, in addition to its mitogenic activity, this growth factor could have a direct stimulatory effect on collagen-gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Epidermal growth factor increases collagen production in granulation tissue by stimulation of fibroblast proliferation and not by activation of procollagen genes

Laato

Kähäri

Niinikoski

et al. 1987

Biochemical Journal

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The effects of epidermal growth factor (EGF) on granulation-tissue formation and collagen-gene expression were studied in experimental sponge-induced granulomas in rats. After daily administration of 5 micrograms of EGF into the sponge, total RNA was extracted from the ingrown granulation tissue at days 4 and 7 and analysed by Northern hybridization for the contents of mRNAs for types I and III procollagens. EGF treatment increased procollagen mRNA, particularly at day 4. To determine whether this elevation was due to increased proliferation of collagen-producing fibroblasts or to activation of collagen-gene expression in these cells, fibroblast cultures were started from granulation tissue and treated with EGF. These experiments confirmed that EGF is a potent mitogen for granuloma fibroblasts in a dose-dependent manner. The effect of EGF treatment on radioactive hydroxyproline production in cultured cells was inhibitory. The decreased rate of collagen synthesis was also indicated by decreased amounts of procollagen mRNAs. The results suggest that the stimulation of wound healing and collagen production by EGF is due to increased fibroblast proliferation, and not to increased expression of type I and III procollagen genes.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Epidermal growth factor increases collagen production in granulation tissue by stimulation of fibroblast proliferation and not by activation of procollagen genes

Laato

Kähäri

Niinikoski

et al. 1987

Biochemical Journal

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“…Its carbonyl stretch vibration was interpreted already in 1967 in favor of a single dominant internally C=O hydrogen bonded conformation in nonpolar solvents. 23 This was confirmed in a microwave study 24 and by quantum chemistry. 25 Microwave, far-IR, and modelling work focused on the coupled torsional dynamics.…”

Section: Pccp Wwwrscorg/pccpmentioning

confidence: 74%

Tailor-made aggregates of α-hydroxy esters in supersonic jets

Borho

Suhm

2004

Phys. Chem. Chem. Phys.

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Nonactin, monactin, dinactin, trinactin, and tetranactin. A Raman spectroscopic study

1975

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SynopsisRaman spectra are reported for crystalline nonactin, monactin, dinactin, trinactin, and tetranactin and their solutions in CC14, CHCl:j, CHxOH, and 4:l (v/v) CH30H:CHCl:I. The macrotetrolide nactins selectively bind a wide variety of cations, and are important model compounds for the study of ion complexation. The conformations of nonactin, monactin, and dinactin in solution are similar. Their conformations are found to be sufficiently open to permit the ester carbonyl groups to form hydrogen bonds with CH3OH; this gives rise to characteristic changes in the vibration frequencies associated with the ester groups. Nonactin, which is the least soluble of the nactins in CH3OH, is also the least effective a t forming hydrogen bonds with CH3OH. The greater ability of the higher nactins to form hydrogen bonds with CH:jOH may be due to the increased inductive effect of ethyl over methyl side chains, which may increase the dipole moment of the ester car-bony1 groups. Spectra of crystalline nonactin, monactin, and tetranactin are fairly similar, while the spectra of dinactin and trinactin comprise a second, distinct family. This is consistent with X-ray crystallographic studies, which show that nonactin and tetranactin form monoclinic crystals, while trinactin is triclinic.

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Asymmetrical Carbonyl Stretching Bands in the Infrared Spectra of Esters.

Cited by 14 publications

References 0 publications

Epidermal growth factor increases collagen production in granulation tissue by stimulation of fibroblast proliferation and not by activation of procollagen genes

Epidermal growth factor increases collagen production in granulation tissue by stimulation of fibroblast proliferation and not by activation of procollagen genes

Tailor-made aggregates of α-hydroxy esters in supersonic jets

Nonactin, monactin, dinactin, trinactin, and tetranactin. A Raman spectroscopic study

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