Asymmetrical Coupling of Enzymatic Systems on Collagen Membranes Application to Multienzyme Electrodes

Coulet, Pierre R.; Bertrand, C.

doi:10.1080/00032717908055709

Cited by 51 publications

(8 citation statements)

References 6 publications

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“…The membrane is then ready for enzyme coupling which must be done immediately. Different types of immobilization involving two or more enzymes could be performed, either at random or in an asymmetrical way with different enzymes on each face of the membrane (Coulet and Bertrand, 1979).…”

Section: Enzyme Immobilization On Collagen Membranesmentioning

confidence: 99%

Design of luminescence photobiosensors

Blum

Gautier

Coulet

1989

J. Biolumin. Chemilumin.

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The potential of immobilized enzyme membranes in biosensors has been explored in our group for several years. Although part of our work has been mainly devoted to electrochemical transducers and oxidases for the design of enzyme electrodes, the demand for ultrasensitive and highly selective sensors led us to consider the use of luminescent enzyme systems associated to optical transduction. When considering the need for operational and reliable biosensors in biotechnology, immobilization and stability of the sensing element still remain, in most cases, an unavoidable problem. We recently proposed a very fast and reliable procedure for preparing enzymatic membranes from Pall (Biodyne Immunoaffinity membranes) supplied in a pre-activated form. Both the firefly and bacterial systems as well as peroxidase for the chemiluminescent determination of various analytes, could be bound to such a support. Based on this approach, a fibre-optic sensor with immobilized enzymes has been designed which permits bio- or chemiluminescent analysis of ATP, NADH or H2O2 respectively. With the NADH-based system, other analytes could be detected using coupled dehydrogenases. This device appears very promising and includes the convenience of both the luminescence sensitivity as well as the handling of the biosensor design.

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Section: Enzyme Immobilization On Collagen Membranesmentioning

confidence: 99%

Design of luminescence photobiosensors

Blum

Gautier

Coulet

1989

J. Biolumin. Chemilumin.

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“…The enzyme electrode created by co-immobilization of amyloglucosidase (which splits D-glucose molecules from the molecule of starch) and glucose oxidase (oxidizing formed D-glucose in the presence of oxygen into D-ghCOnOlaCtOne, resp. D-gluconic acid had hydrogen peroxide), ist the simplest sensor of this type [4,5]. Many authors [6,7,8] have dealt with the co-immobilization of the above enzymes due to the biosensor preparation for the determination of starch (or maltose) either with the connection of a Clark-type oxygen sensor [4,9] or an amperometric hydrogen peroxide sensor [5,10].…”

Section: Introductionmentioning

confidence: 99%

“…D-gluconic acid had hydrogen peroxide), ist the simplest sensor of this type [4,5]. Many authors [6,7,8] have dealt with the co-immobilization of the above enzymes due to the biosensor preparation for the determination of starch (or maltose) either with the connection of a Clark-type oxygen sensor [4,9] or an amperometric hydrogen peroxide sensor [5,10]. At this work we used the methodology […”

Section: Introductionmentioning

confidence: 99%

Biosensor for Determination of Starch

1993

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Biosensor for determination of starch was prepared by fixing a carrier with immobilized enzyme system on the tip of a Clark‐type oxygen sensor. Enzymes amyloglucosidase, mutarotase, glucose oxidase and catalase were co‐immobilized either on the partially hydrolyzed nylon net or on the activated collagen membrane. In both cases the covalent bond was made in the presence of glutaraldehyde and cyclohexyl isocyanide. The prepared biosensors were compared from the viewpoint of a specific activity of the immobilized amyloglucosidase, of a linear response area of the biosensor to the substrate concentration, of the pH and temperature of the reaction mixture and of the biosensor stability. Biosensors prepared were used for the determination of starch in the samples of cultivaiting media for the monensin production.

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“…Spectrophotonietric methods have been widely used until now [ 1, 21, but they entail tedious procedures and are time-consuming. On the other hand, several electrochemical sensor or flow-injection methods using inimobilized enzyme or free-enzyme solutions have been reported to perform a convenient measurement o f cholesterol [3][4][5][6]. A relatively large sample volumc IS needed in amperometry, however, and an analytical pump must be used to flow the carrier solution at a constant rate.…”

Section: Introductionmentioning

confidence: 99%

Biocoulometry of cholesterol using porous carbon felt electrodes

et al. 1991

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Free and total cholesterols in human serum have been determined by controlled-potential coulometry with porous carbon felt electrodes. In such a coulometric cell, a small amount of sample solution was dropped on the carbon felt dipped into electrolyte containing enzymes and a mediator. Hydrogen peroxide is produced by the enzymatic reaction of cholesterol catalyxd by cholesterol oxidase, and the complete electroreduction of hemcyanoferrate( 111) ions [produced by the reaction of hexacynoferrate( 11) ions and hydrogen peroxide catalyzed by peroxidase] was carried out under controlled-potential conditions. In the case of determining total cholesterol in human serum, cholesterol esters were converted to free cholesterol by cholesterol esterase reaction prior to the addition of the sample solution into electrolyte. The conversion ratios of free cholesterol to hydrogen peroxide were found to be 85-89% (KSD < 6%). The electrolysis time o f one sample was less than 5 minutes. Immobilization of cholesterol oxidase to the carbon felt surface was attempted; the activity of cholesterol oxidase immobilized by the diazo-coupling method began to decrease 3 days after the immobilization.

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Asymmetrical Coupling of Enzymatic Systems on Collagen Membranes Application to Multienzyme Electrodes

Cited by 51 publications

References 6 publications

Design of luminescence photobiosensors

Design of luminescence photobiosensors

Biosensor for Determination of Starch

Biocoulometry of cholesterol using porous carbon felt electrodes

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