Biosensor for Determination of Starch

Abstract

Biosensor for determination of starch was prepared by fixing a carrier with immobilized enzyme system on the tip of a Clark‐type oxygen sensor. Enzymes amyloglucosidase, mutarotase, glucose oxidase and catalase were co‐immobilized either on the partially hydrolyzed nylon net or on the activated collagen membrane. In both cases the covalent bond was made in the presence of glutaraldehyde and cyclohexyl isocyanide. The prepared biosensors were compared from the viewpoint of a specific activity of the immobilized amyloglucosidase, of a linear response area of the biosensor to the substrate concentration, of the pH and temperature of the reaction mixture and of the biosensor stability. Biosensors prepared were used for the determination of starch in the samples of cultivaiting media for the monensin production.

Networked Enzymatic Logic Gates with Filtering: New Theoretical Modeling Expressions and Their Experimental Application

“…We use it as a model system to explore ideas of parametrizing and optimizing small-network functioning. Furthermore, the present system is of interest because it consists of steps similar to those which have also been incorporated in enzymatic cascades devised for biosensor application involving detection of maltose or starch. − The (bio)chemical processes in our system are shown in Scheme . The first step functions as an AND logic gate with two variable inputs: maltose, which we select as logic Input 1, and phosphate, selected as Input 2.…”

Biosensors based on modified screen-printed enzyme electrodes for monitoring of fermentation processes

“…For example, it is characterized by the broader interval of the determinable concentrations of glucose, lactate, and starch compared to the described prototypes [9,10,21,22]. In addition, the developed biosensor system, in contrast to the known analogs, allows selective determination of the content of starch, glucose, lactate, and ethanol in fermentation media.…”

“…The starch content can be determined by the biosensor method using as biological component a mixture of two enzymes: glucose oxidase and amylase [9]. One of such examples is an expendable screen-printed electrode for simultaneous determination of starch and glucose in foodstuffs [10].…”

Theoretical modeling expressions for networked enzymatic signal processing steps as logic gates optimized by filtering

“…In fact, this cascade consists of steps similar to those which have also been incorporated in enzymatic biosensors involving detection of maltose or starch. [80][81][82][83][84] The first step functions as an AND logic gate. Its two inputs are maltose (selected as logic Input 1) and phosphate (Input 2).…”