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This research shows that high-performance biosensors can be produced by modification of screen-printed electrodes with enzymes and conducting hydrogel based on sol-gel matrix and single-walled carbon nanotubes. Tetraethoxysilane, dimethyldiethoxysilane and polyvinyl alcohol were used as the sol-gel matrix basis. Modified SWCNT provide direct electron transfer during glucose oxidation, as confirmed by cyclic voltammetry. The developed conducting sol-gel screen-printed electrodes can determine glucose within the concentration range 0.045-1.04 mM. The developed biosensor is not only in pace with its world analogues but even exceeds them by some analytical and metrological properties. The developed conducting sol-gel biosensor was used to measure the concentration of glucose in blood. The test results differed only insignificantly from those received with the help of standard glucose meter.

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Biosensor array based on modifi ed screen-printed electrodes containing enzymes (glucose oxidase, alcohol oxidase, lactate oxidase, mixture of glucose oxidase and γ-amylase) immobilized in a membrane of cross-linked bovine serum albumin was developed for the determination of the content of glucose, ethanol, lactic acid, and starch in fermentation media. The analytical and metrological characteristics of the developed biosensor system were determined. Samples of the fermentation mass and of alcoholic and fermented milk products were analyzed. Statistical analysis of the results obtained showed that the results of determining the content of glucose, ethanol, lactic acid, and starch with the developed biosensor system and by reference methods differed insignifi cantly. The developed biosensor system is considered as a base for fabricating a prototype of an analyzer for use in fermentation biotechnology.

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