Screen-printed electrodes modified with glucose oxidase immobilized in hybrid organosilicon sol-gel matrix

Kamanin, S. S.; Arlyapov, V. A.; Rogova, Tatiana; Reshetilov, A. N.

doi:10.1134/s0003683814090038

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…This work not only demonstrates this phenomenon, but also shows an example of its application -the creation of a biosensor directly at the analysis site (fish tank). It is important to note that the analytical response of the system to the substrate injections is represented not by the readings of one sensor layer, as in traditionally used microbial biosensors [20][21][22], but by the difference between the readings of two technically identical sensors, differing only by the type and amount of nanodiamonds anchored to surface. The coating with DNDamine demonstrates the suppression of fouling, and such a sensor actually shows the oxygen concentration in the bulk volume, while the coating with DNDamine+chl determines the local oxygen depletion in the biofilm layer.…”

Section: Discussionmentioning

confidence: 99%

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Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design

et al. 2021

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The development of different methods for tuning surface properties is currently of great interest. The presented work is devoted to the use of modified nanodiamonds to control the wetting and biological fouling of polymers using optical sensors as an example. We have shown that, depending on the type of modification and the amount of nanodiamonds, the surface of the same fluorinated polymer can have both bactericidal properties and, on the contrary, good adhesion to the biomaterial. The precise control of wetting and biofouling properties of the surface was achieved by the optimization of the modified nanodiamonds thermal anchoring conditions. In vitro and in vivo tests have shown that the fixation of amine functional groups leads to inhibition of biological activity, while the presence of a large number of polar groups of mixed composition (amide and acid chloride) promotes adhesion of the biomaterial and allows one to create a biosensor on-site. A comprehensive study made it possible to establish that in the first 5 days the observed biosensor response is provided by cells adhered to the surface due to the cell wall interaction. On the 7th day, the cells are fixed by means of the polysaccharide matrix, which provides much better retention on the surface and a noticeably greater response to substrate injections. Nevertheless, it is important to note that even 1.5 h of incubation is sufficient for the formation of the reliable bioreceptor on the surface with the modified nanodiamonds. The approach demonstrated in this work makes it possible to easily and quickly isolate the microbiome on the surface of the sensor and perform the necessary studies of its substrate specificity or resistance to toxic effects.

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Section: Discussionmentioning

confidence: 99%

“…Carbon nanomaterials are actively used to create sensors, for example, for express estimation of biochemical oxygen demand (BOD) [20]. The good adhesion of the biomaterial to the working surface is the key property for such systems [21,22].…”

Section: Introductionmentioning

confidence: 99%

Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design

et al. 2021

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“…Glucose oxidase immobilized on bioelectrodes, which are modified with such hybrid composition, shows high stability and allows increasing the range of detected glucose concentration (1.0−5.9 μM) compared with agar gel electrodes (3.6−6.3 μM). 164 Optimization of silica-based sol−gel lipase encapsulation is achieved by mediation with PEI. PEI coating prevents the direct interaction between enzyme and surface, protecting the native structure.…”

Section: ■ Hybridsmentioning

confidence: 99%

“…A hybrid silica-organic/PVA composition may be applied for production of biosensors, which are sensitive to alcohols and glucose, to analyze wine products. Glucose oxidase immobilized on bioelectrodes, which are modified with such hybrid composition, shows high stability and allows increasing the range of detected glucose concentration (1.0–5.9 μM) compared with agar gel electrodes (3.6–6.3 μM) …”

Section: Hybridsmentioning

confidence: 99%

Application of Immobilized Enzymes in Food Industry

Yushkova

Nazarova

Matyuhina

et al. 2019

J. Agric. Food Chem.

145

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Enzymes are macromolecular biocatalysts, widely used in food industry. In applications, enzymes are often immobilized on inert and insoluble carriers, which increase their efficiency due to multiple reusability. The properties of immobilized enzymes depend on the immobilization method and the carrier type. The choice of the carrier usually concerns the biocompatibility, chemical and thermal stability, insolubility under reaction conditions, capability of easy regeneration and reusability, as well as cost efficiency. In this review, we provide an overview of various carriers for enzyme immobilization, with the primary focus on food industry.

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“…For example, several models of screen-printed electrodes based on glucose oxidase for the glucose determination in the average interval 0.01-1 mM have been described [5]. The possibility of developing a biosensor for glucose determination on the basis of screen-plated electrodes modified with Prussian Blue and glucose oxidase immobilized into a hybrid organosilicon sol-gel matrix was demonstrated previously [6]. A biosensor based on a screen-printed electrode modifi ed with alcohol oxidase for the ethanol determination in the interval 0.05-1 mM has been reported [7].…”

mentioning

confidence: 98%

Biosensors based on modified screen-printed enzyme electrodes for monitoring of fermentation processes

et al. 2015

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Biosensor array based on modifi ed screen-printed electrodes containing enzymes (glucose oxidase, alcohol oxidase, lactate oxidase, mixture of glucose oxidase and γ-amylase) immobilized in a membrane of cross-linked bovine serum albumin was developed for the determination of the content of glucose, ethanol, lactic acid, and starch in fermentation media. The analytical and metrological characteristics of the developed biosensor system were determined. Samples of the fermentation mass and of alcoholic and fermented milk products were analyzed. Statistical analysis of the results obtained showed that the results of determining the content of glucose, ethanol, lactic acid, and starch with the developed biosensor system and by reference methods differed insignifi cantly. The developed biosensor system is considered as a base for fabricating a prototype of an analyzer for use in fermentation biotechnology.

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Screen-printed electrodes modified with glucose oxidase immobilized in hybrid organosilicon sol-gel matrix

Cited by 11 publications

References 20 publications

Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design

Modified Nanodiamonds as a Means of Polymer Surface Functionalization. From Fouling Suppression to Biosensor Design

Application of Immobilized Enzymes in Food Industry

Biosensors based on modified screen-printed enzyme electrodes for monitoring of fermentation processes

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