Asymmetry in traction forces produced by migrating preadipocytes is bounded to 33%

Weihs, Daphne

doi:10.1016/j.medengphy.2016.05.013

Cited by 15 publications

(7 citation statements)

References 32 publications

(40 reference statements)

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“…In previous studies which discussed the motility speed of preadipocytes or adipose-derived mesenchymal stem cells, it was revealed that the average cell speed values were in the same order of magnitude as the results presented here [20–100 ( μ m/h)] which confirms our findings (Feng et al, 2014; Abuhattum & Weihs, 2016).…”

Section: Discussionsupporting

confidence: 92%

Adipocytes Migration is Altered Through Differentiation

et al. 2019

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Adipogenesis is a developmental process in which an elongated preadipocyte differentiates to a round adipocyte along with the accumulation of lipid droplets. In the present study, we focus on the study of cell motility at the single-cell level, toward expanding our knowledge regarding the cytoskeleton alteration during differentiation; since-cell motility is mediated by cytoskeletal components. We used the holographic-microscopy live imaging technique to evaluate, for the first time in the literature, differences between the motility of nondifferentiated preadipocytes and differentiated mature adipocytes in living cell cultures over time. We revealed that mean motility speed of preadipocytes was significantly higher (fourfold) than that of adipocytes, and that the movement of preadipocytes is less consistent and more extensive. Furthermore, we found that preadipocytes tend to migrate to farther distances, while mature adipocytes remain relatively close to their original location. The results presented here are in agreement with the fact that the cytoskeleton of adipocytes is altered during differentiation and similarly, points to the fact that the cell-sensing mechanisms are changing during differentiation. Our research paves the way to gain better insights of the differentiation process and its implications on larger scale systems in the context of obesity.

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Section: Discussionsupporting

confidence: 92%

Adipocytes Migration is Altered Through Differentiation

et al. 2019

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“…Based on the 10,000 samples, Fig. 13 a shows the corresponding histogram, which looks like a lognormal chart and fits from a qualitative point of view with the experimental results by Abuhattum and Weihs ( 2016 ), where the migration speeds of single preadipocytes without chemoattractants follow a lognormal distribution. A cumulative percentage of the number of occurrences regarding the cell penetration time is plotted in Fig.…”

Section: The Numerical Simulationssupporting

confidence: 53%

A phenomenological model for cell and nucleus deformation during cancer metastasis

Chen

Weihs

Dijk

et al. 2018

Biomech Model Mechanobiol

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Cell migration plays an essential role in cancer metastasis. In cancer invasion through confined spaces, cells must undergo extensive deformation, which is a capability related to their metastatic potentials. Here, we simulate the deformation of the cell and nucleus during invasion through a dense, physiological microenvironment by developing a phenomenological computational model. In our work, cells are attracted by a generic emitting source (e.g., a chemokine or stiffness signal), which is treated by using Green’s Fundamental solutions. We use an IMEX integration method where the linear parts and the nonlinear parts are treated by using an Euler backward scheme and an Euler forward method, respectively. We develop the numerical model for an obstacle-induced deformation in 2D or/and 3D. Considering the uncertainty in cell mobility, stochastic processes are incorporated and uncertainties in the input variables are evaluated using Monte Carlo simulations. This quantitative study aims at estimating the likelihood for invasion and the length of the time interval in which the cell invades the tissue through an obstacle. Subsequently, the two-dimensional cell deformation model is applied to simplified cancer metastasis processes to serve as a model for in vivo or in vitro biomedical experiments.Electronic supplementary materialThe online version of this article (10.1007/s10237-018-1036-5) contains supplementary material, which is available to authorized users.

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“…These cells provide simplified models for the cell types prevalent at susceptible superficial and deep tissue sites, which would be involved in the PU formation process. The undifferentiated cells are also migratory and would subsequently be able to migrate to repair any micro‐scale damage that may form …”

Section: Methodsmentioning

confidence: 99%

Sodium pyruvate pre‐treatment prevents cell death due to localised, damaging mechanical strains in the context of pressure ulcers

Alvarez-Elizondo

Barenholz-Cohen

Weihs

2019

International Wound Journal

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We demonstrate sodium pyruvate (NaPy) pre‐treatment as a successful approach for pressure ulcer (PU) prevention by averting their aetiological origin—cell‐level damage and death by large, sustained mechanical loads. We evaluated the NaPy pre‐treatment effect on permeability changes in the cell's plasma membrane (PM) following application of in vitro damaging‐level strains. Fibroblasts or myoblasts, respectively, models for superficial or deep‐tissue damage were grown in 0 or 1 mM NaPy, emulating typical physiological or cell culture conditions. Cells were pre‐treated for 4 hours with 0 to 5 mM NaPy prior to 3‐hour sustained, damaging‐level loads (12% strain). PM permeability was quantified by the cell uptake of small (4 kDa), fluorescent dextran compared with unstrained control using fluorescence‐activated cell sorting (FACS). Pre‐treatment with 1 mM, and especially 5 mM, NaPy significantly reduces damage to PM integrity. Long‐term NaPy pre‐exposure can improve protective treatment, affecting fibroblasts and myoblasts differently. Pre‐treating with NaPy, a natural cell metabolite, allows cells under damaging‐level mechanical loads to maintain their PM integrity, that is, to avoid loss of homeostasis and inevitable, eventual cell death, by preventing initial, microscale stages of PU formation. This pre‐treatment may be applied prior to planned periods of immobility, for example, planned surgery or transport, to prolong safe time in a position by preventing initial cell damage that can cascade and lead to PU formation.

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Asymmetry in traction forces produced by migrating preadipocytes is bounded to 33%

Cited by 15 publications

References 32 publications

Adipocytes Migration is Altered Through Differentiation

Adipocytes Migration is Altered Through Differentiation

A phenomenological model for cell and nucleus deformation during cancer metastasis

Sodium pyruvate pre‐treatment prevents cell death due to localised, damaging mechanical strains in the context of pressure ulcers

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