Asymmetry of lipid dynamics in human erythrocyte membranes studied with impermeant fluorophores

Schachter, David; Cogan, Uri; Abbott, Richard E.

doi:10.1021/bi00525a018

Cited by 92 publications

(39 citation statements)

References 49 publications

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“…In erythrocytes, differences in fluidity between the two monolayers have not been consistently observed. Some studies have reported that the outer monolayer is less fluid (Seigneuret et al, 1984;Chabanel et al, 1985), whereas other studies have found the opposite (Cogan and Schachter, 1981;Schachter et al, 1983). The finding that the inner monolayer of synaptic plasma membrane isolated from rat brain (SPM) is less fluid than the outer monolayer is consistent with data showing that the SPM inner monolayer contains approximately 7-times as much cholesterol as the outer monolayer (Wood et al, 1990).…”

Section: Effect Of Chlorpromazine · Hcl On the Rate And Range Of Transupporting

confidence: 62%

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Jang

Jeong²,

Ahn³

et al. 2004

BMB Reports

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Fluorescence probes located in different membrane regions were used to evaluate the effects of chlorpromazine · HCl on structural parameters (transbilayer lateral mobility, annular lipid fluidity, protein distribution, and lipid bilayer thickness) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. The experimental procedure was based on the selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups, radiationless energy transfer from the tryptophan of membrane proteins to Py-3-Py, and energy transfer from Py-3-Py monomers to 1-anilinonaphthalene-8-sulfonic acid (ANS). In this study, chlorpromazine · HCl decreased the lateral mobility of Py-3-Py in a concentration dependent-manner, showed a greater ordering effect on the inner monolayer than on the outer monolayer, decreased annular lipid fluidity in a dose dependent-manner, and contracted the membrane lipid bilayer. Furthermore, the drug was found to have a clustering effect on membrane proteins.

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Section: Effect Of Chlorpromazine · Hcl On the Rate And Range Of Transupporting

confidence: 62%

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Jang

Jeong²,

Ahn³

et al. 2004

BMB Reports

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“…In this regard, however, it should be noted that, as evaluated by steadystate fluorescence polarization of lipid fluorophores, "fluidity" has usually been assessed via the fluorescence anisotropy r, without further resolution of the components that determine r. Recent studies with the rodlike fluorophore DPH, however, have demonstrated that the rotations ofthis probe are hindered in both artificial and biological membranes (26)(27)(28). Therefore, the fluorescence anisotropy of such a fluorophore is not adequately described by the Perrin equation but by a modified relationship (29,30) (33). Final molar ratios of probe/lipid ranged from 0.001 to 0.002 and the anisotropy differences noted in these studies could not be ascribed to differences in probe concentration in the preparations.…”

Section: Methodsmentioning

confidence: 99%

Premalignant alterations in the lipid composition and fluidity of colonic brush border membranes of rats administered 1,2 dimethylhydrazine.

Brasitus¹,

Dudeja²,

Dahiya³

1986

J. Clin. Invest.

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Dimethylhydrazine (DMH) is a potent procarcinogen with selectivity for the colon. To determine whether alterations in the lipid composition and fluidity of rat colonic brush border membranes existed before the development of DMH-induced colon cancer, rats were injected s.c. with this agent (20 mg/kg body weight per wk) or diluent for 5, 10, and 15 wk. Animals were killed at these time periods and brush border membranes were prepared from proximal and distal colonocytes of each group. The "static" and "dynamic" components of fluidity of each membrane were then assessed, by steady-state fluorescence polarization techniques using limiting hindered fluorescence anisotropy and order parameter values of the fluorophore 1,6 diphenyl-1,3,5-hexatriene (DPH) and fluorescence anisotropy values of DL-249-anthroyl) stearic acid and DL-12(9-anthroyl) stearic acid, respectively. Membrane lipids were extracted and analyzed by thin-layer chromatography and gas-liquid chromatography. Phospholipid methylation activity in these membranes was also measured using S-adenosyl-L-methionine as the methyl donor.The results of these studies demonstrate that: (a) the lipid composition and both components of fluidity of proximal DMHtreated and control membranes and their liposomes were similar at all time periods examined; (b) at 5, 10, and 15 wk the "dynamic component of fluidity" of distal DMH-treated membranes and their liposomes was found to be higher, similar, and lower, respectively, than their control counterparts; (c) the "static component of fluidity" of distal DMH-treated membranes and their liposomes, however, was similar to control preparations at all three time periods; and (d) alterations in the lipid composition and phospholipid methylation activities appeared to be responsible for these differences in the "dynamic component of fluidity" at these various time periods.

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“…For animal, bacterial, and viral systems, various membranes are known to have a characteristic asymmetry of fluidity (e.g., Cogan and Schachter, 1981;Foley et al, 1986;Dudeja et al, 1991;Kitagawa et al, 1991Kitagawa et al, , 1998Julien et al, 1993;Müller et al, 1994;Schroeder et al, 1995;Igbavboa et al, 1996). Variation of lipid fluidity between individual hemileaflets may therefore be a general feature of biological membranes.…”

Section: Introductionmentioning

confidence: 99%

Chilling Tolerance in Arabidopsis Involves ALA1, a Member of a New Family of Putative Aminophospholipid Translocases

Gomès¹,

Jakobsen²,

Axelsen³

et al. 2000

Plant Cell

147

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The lipid composition of membranes is a key determinant for cold tolerance, and enzymes that modify membrane structure seem to be important for low-temperature acclimation. We have characterized ALA1 (for aminophospholipid ATPase1 ), a novel P-type ATPase in Arabidopsis that belongs to the gene family ALA1 to ALA11 . The deduced amino acid sequence of ALA1 is homologous with those of yeast DRS2 and bovine ATPase II, both of which are putative aminophospholipid translocases. ALA1 complements the deficiency in phosphatidylserine internalization into intact cells that is exhibited by the drs2 yeast mutant, and expression of ALA1 results in increased translocation of aminophospholipids in reconstituted yeast membrane vesicles. These lines of evidence suggest that ALA1 is involved in generating membrane lipid asymmetry and probably encodes an aminophospholipid translocase. ALA1 complements the cold sensitivity of the drs2 yeast mutant. Downregulation of ALA1 in Arabidopsis results in cold-affected plants that are much smaller than those of the wild type. These data suggest a link between regulation of transmembrane bilayer lipid asymmetry and the adaptation of plants to cold. INTRODUCTIONLow temperature is one of the major environmental factors limiting plant growth. Cold-sensitive plants are injured and stunted in growth when temperatures are well below those for normal growth but still above the freezing point (reviewed in Nishida and Murata, 1996;Pearce, 1999;Thomashow, 1999). Many tropical or subtropical plant species, including a large number of crops such as tomato, rice, cotton, cucumber, and maize, are susceptible to chilling injury, and substantial losses in productivity result from the inability of such crops to withstand cold stress. Normal functioning of integral membrane proteins such as transporters and receptor proteins depends on the fluidity of the membrane, which is strongly influenced at a given temperature by its lipid composition (Squier et al., 1988;Gasser et al., 1990; reviewed in Hazel, 1995).A major factor determining the fluidity of lipid membranes is the degree of unsaturation of membrane lipids. Thus, membranes with unsaturated acyl chains in phospholipids remain fluid at lower temperatures than do membranes with saturated lipids (Kates et al., 1984;Cevc, 1991;Cossins, 1994). One of the best-documented responses of plants to chilling stress is the increase in polyunsaturated acyl chains of membrane phospholipids, which allows membrane fluidity to be maintained (Hugly and Somerville, 1992;Nishida and Murata, 1996). This response indicates that after transfer to the cold, plants may increase the amount of unsaturated lipids by upregulating the activity of desaturase enzymes. Accordingly, expression in tobacco of a desaturase gene from a cyanobacterium results in increasing membrane lipid unsaturation in most membrane lipids concomitant with an increase in chilling tolerance (IshizakiNishizawa et al., 1996).The proportion of unsaturated fatty acids in the lipid acyl chains is particularly hig...

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Asymmetry of lipid dynamics in human erythrocyte membranes studied with impermeant fluorophores

Cited by 92 publications

References 49 publications

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Effects of Chlorpromazine·HCl on the Structural Parameters of Bovine Brain Membranes

Premalignant alterations in the lipid composition and fluidity of colonic brush border membranes of rats administered 1,2 dimethylhydrazine.

Chilling Tolerance in Arabidopsis Involves ALA1, a Member of a New Family of Putative Aminophospholipid Translocases

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