Asymptomatic malaria infections among foreign migrant workers in Thailand

Kritsiriwuthinan, Kanyanan; Ngrenngarmlert, Warunee

doi:10.1016/s1995-7645(11)60146-6

Cited by 24 publications

(21 citation statements)

References 25 publications

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“…Berdasarkan penelitian sebelum dan uji perhitungan hipotesis odds ratio, diperoleh sampel minimal yang dibutuhkan dalam penelitian ini sebanyak 240 orang, kelompok kasus dan kontrol yang disertakan dengan perbandingan 1 : 2,80 orang kelompok kasus dan 160 orang kelompok kontrol. 4,[6][7][8] Analisis data bivariat menggunakan uji binary logistic dan analisis data multivariat menggunakan uji regresi logistik berganda.…”

Section: Metodeunclassified

Determinan Kejadian Malaria di Wilayah Endemis

Hasyim¹,

Camelia²,

Fajar³

2014

Kesmas: National Public Health Journal

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AbstrakKabupaten Lahat adalah salah satu wilayah endemis malaria di Sumatera Selatan dengan prevalensi 16,4% dan Annual Malaria Incidence 22,08. Tujuan penelitian ini untuk mengetahui faktor risiko lingkungan dengan kejadian malaria. Faktor risiko lingkungan genangan air (breeding place) berhubungan dengan kejadian malaria dengan nilai p= 0,000. Analisis multivariat menemukan determinan utama kejadian malaria adalah breeding place di sekitar rumah responden dengan odds ratio (OR) = 5,034 dan 95% CI = 2,65 _ 9,56. Responden yang tinggal di sekitar breeding place berisiko 5,03 kali lebih besar untuk menderita malaria dibandingkan dengan responden yang di sekitar rumah tidak terdapat breeding place setelah dikontrol variabel jarak rumah ke breeding place, ventilasi rumah, penggunaan kelambu, penggunaan obat anti nyamuk, dan kebiasaan keluar rumah pada malam hari. Kata kunci: Daerah endemis, lingkungan genangan air, malaria AbstractLahat district is one of the malaria endemic area in South Sumatra Province with a prevalence of 16.4% and Annual Malaria Incidence of 22.08. The case control reports were carried out of 240 respondents. This study aimed to understand the relationship among of environmental risk factors with the incidence of malaria. After primary data collection followed by processing and data analysis in a multimedia laboratory. There was association between breeding place and malaria cases (p value= 0.000). The results of multivariate analysis of variables revealed the determinant risk was breeding place, with OR = 5.034 and CI 95%= 2.65 _ 9.56. Respondents who live around the breeding place has 5.034 times chance of affected malaria compared with respondents around the house there are no breeding place after the controlled distance to the breeding place house, use of mosquito nets, use of anti-mosquito, and habits out of the house at night variables.

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Section: Metodeunclassified

Determinan Kejadian Malaria di Wilayah Endemis

Hasyim¹,

Camelia²,

Fajar³

2014

Kesmas: National Public Health Journal

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“…In countries where malaria is endemic, a significant proportion of P. falciparum infections are asymptomatic or subclinical. Asymptomatic carriage levels detected by microscopy and/or other methods have been reported to be as high as 39%, 8.4%, and 1.36 to 7.7% in African countries, India, and Thailand, respectively (6)(7)(8)(9)(10)(11)(12). However, surveillance data from Myanmar nationwide are not available to date.…”

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confidence: 99%

Comparison of Microscopy, Nested-PCR, and Real-Time-PCR Assays Using High-Throughput Screening of Pooled Samples for Diagnosis of Malaria in Asymptomatic Carriers from Areas of Endemicity in Myanmar

et al. 2014

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Asymptomatic infection is an important obstacle for controlling disease in countries where malaria is endemic. Because asymptomatic carriers do not seek treatment for their infections, they can have high levels of gametocytes and constitute a reservoir available for new infection. We employed a sample pooling/PCR-based molecular detection strategy for screening malaria infection in residents from areas of Myanmar where malaria is endemic. Blood samples (n ‫؍‬ 1,552) were collected from residents in three areas of malaria endemicity (Kayin State, Bago, and Tanintharyi regions) of Myanmar. Two nested PCR and real-time PCR assays showed that asymptomatic infection was detected in about 1.0% to 9.4% of residents from the surveyed areas. The sensitivities of the two nested PCR and real-time PCR techniques were higher than that of microscopy examination (sensitivity, 100% versus 26.4%; kappa values, 0.2 to 0.5). Among the three regions, parasite-positive samples were highly detected in subjects from the Bago and Tanintharyi regions. Active surveillance of residents from regions of intense malaria transmission would reduce the risk of morbidity and mitigate transmission to the population in these areas of endemicity. Our data demonstrate that PCRbased molecular techniques are more efficient than microscopy for nationwide surveillance of malaria in countries where malaria is endemic.

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“…Currently, the detection of P. falciparum infection has required the method that has high sensitivity and specificity, especially for a screening of the asymptomatic patients and blood donation [21]. PCR has been developed to detect the malaria parasite for increasing the sensitivity and specificity, including semi-nested PCR [17], nested PCR [13,23,15], and real-time PCR [18].…”

Section: Discussionmentioning

confidence: 99%

“…Several PCR-based assays include conventional PCR [10], multiplex PCR [11], loop-mediated isothermal amplification (LAMP) [12], nested PCR [13][14][15][16], semi-nested PCR [17], and real-time PCR [18]. PCR has shown to be good alternative methods, because of the high sensitivity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Two Nested PCR-Based Diagnostic Assays for Plasmodium Falciparum Infection

Kritsiriwuthinan

Choosang

Sunantaraporn

2017

Asian J Pharm Clin Res

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Objective: The majority of malaria cases and deaths are caused by Plasmodium falciparum. The rapid and accurate diagnosis is very important for malaria treatment and control. The aim of this study was to evaluate two nested polymerase chain reaction (PCR)-based methods (protocol A and protocol B) for P. falciparum infection, diagnosis in Thailand.Methods: A total of 90 dried blood spot samples were investigated. The samples composed of P. falciparum-, Plasmodium vivax-infected blood and normal human blood samples. The microscopic examination was used as gold standard. Results:The results showed the sensitivity of 100/83.33%, specificity of 100/100%, and accuracy of 100/94.44% for protocol A and protocol B, respectively. The analytical sensitivity of protocol A and protocol B was 0.625 and 6.25 parasites/µl, respectively. The comparison among microscopic examination, protocol A and protocol B by statistical analysis, found that they were not a significant difference. The agreements between each method were good. The kappa value between protocol A and protocol B was 0.87, protocol A and microscopy was 1.00, and protocol B and microscopy was 0.87. Conclusion:The results demonstrated that protocol A should be used for further development of P. falciparum diagnosis in Thailand, especially in case of low parasitemia such as asymptomatic infection and for screening blood donors.

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Asymptomatic malaria infections among foreign migrant workers in Thailand

Abstract: These findings indicate a high risk of malaria transmission. Therefore active malaria surveillance by using molecular methods with more sensitive and specific than microscopy should be considered for malaria control in foreign migrant workers.

Cited by 24 publications

References 25 publications

Determinan Kejadian Malaria di Wilayah Endemis

Determinan Kejadian Malaria di Wilayah Endemis

Comparison of Microscopy, Nested-PCR, and Real-Time-PCR Assays Using High-Throughput Screening of Pooled Samples for Diagnosis of Malaria in Asymptomatic Carriers from Areas of Endemicity in Myanmar

Evaluation of Two Nested PCR-Based Diagnostic Assays for Plasmodium Falciparum Infection

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