AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum

Kim, Sang Woo; Yamaoka, Yasuyo; Ono, Hideki; Kim, Hanul; Shim, Donghwan; Maeshima, Masayoshi; Martinoia, Enrico; Cahoon, Edgar B.; Nishida, Ikuo; Lee, Youngsook

doi:10.1073/pnas.1214159110

Cited by 113 publications

(92 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AtABCA9, an Arabidopsis ER-localized A-type ABC transporter was shown to function in the process. [41] The atabca9 null mutant reduced seed TAG content by 35% and the developing atabca9 seeds incorporated 35% less 14 C-labelled-oleoyl-CoA into TAG compared with WT. Moreover, overexpression of AtABCA9 enhanced TAG deposition by up to 40%.…”

Section: Plastid-er Fa Transportmentioning

confidence: 95%

“…Moreover, overexpression of AtABCA9 enhanced TAG deposition by up to 40%. [41] This indicates that AtABCA9 may transport FA substrates for TAG biosynthesis at the ER during the seed-filling stage and some other transporters may be responsible for FAs transport from the cytosol into the ER.…”

Section: Plastid-er Fa Transportmentioning

confidence: 97%

“…The mechanisms of FA transport from the plastids to cytosol, and then to the ER, have been studied. [40,41] A FA export 1 (FAX1), located in the inner envelope of the chloroplast, plays an essential role in the transportation of FAs out of the chloroplast. The mutation of FAX1 results in pleiotropic effects on developments and metabolism, including retarded growth, impaired male fertility, reduced TAG and FA-derived phospholipids but increased accumulation of plastidic PG production.…”

Section: Plastid-er Fa Transportmentioning

confidence: 99%

See 2 more Smart Citations

Transport and transcriptional regulation of oil production in plants

Manan

Chen

She

et al. 2016

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

Triacylglycerol (TAG) serves as an energy reservoir and phospholipids as build blocks of biomembrane to support plant life. They also provide human with foods and nutrients. Multi-compartmentalized biosynthesis, trafficking or cross-membrane transport of lipid intermediates or precursors and their regulatory mechanisms are not fully understood. Recent progress has aided our understanding of how fatty acids (FAs) and phospholipids are transported between the chloroplast, the cytoplasm, and the endoplasmic reticulum (ER), and how the ins and outs of lipids take place in the peroxisome and other organelles for lipid metabolism and function. In addition, information regarding the transcriptional regulation network associated with FA and TAG biosynthesis has been further enriched. Recent breakthroughs made in lipid transport and transcriptional regulation has provided significant insights into our comprehensive understanding of plant lipid biology. This review attempts to highlight the recent progress made on lipid synthesis, transport, degradation, and their regulatory mechanisms. Metabolic engineering, based on these knowledge-powered technologies for production of edible oils or biofuels, is reviewed. The biotechnological application of metabolic enzymes, transcription factors and transporters, for oil production and composition improvement, are discussed in a broad context in order to provide a fresh scenario for researchers and to guide future research and applications.ARTICLE HISTORY

show abstract

Section: Plastid-er Fa Transportmentioning

confidence: 95%

Section: Plastid-er Fa Transportmentioning

confidence: 97%

Section: Plastid-er Fa Transportmentioning

confidence: 99%

See 1 more Smart Citation

Transport and transcriptional regulation of oil production in plants

Manan

Chen

She

et al. 2016

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

show abstract

“…They fulfil a range of functions within the plant and roles have been established in the transport of hormones, lipids, metals, secondary metabolites, and xenobiotics (reviewed in 114). The first member of the ABCA subfamily characterized, AtABCA9, has recently been demonstrated to mediate the transport of fatty acids for lipid synthesis in the endoplasmic reticulum (115). A number of members of the ABCB subfamily are auxin efflux carriers (116), whereas AtABCB14 is a malate importer (117; as opposed to the protein expected to export malate out of the cytosol and into the symbiosome).…”

Section: Soybean Symbiosome Proteomementioning

confidence: 99%

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Clarke

Loughlin

Gavrin

et al. 2015

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Legumes form a symbiosis with rhizobia in which the plant provides an energy source to the rhizobia bacteria that it uses to fix atmospheric nitrogen. This nitrogen is provided to the legume plant, allowing it to grow without the addition of nitrogen fertilizer. As part of the symbiosis, the bacteria in the infected cells of a new root organ, the nodule, are surrounded by a plant-derived membrane, the symbiosome membrane, which becomes the interface between the symbionts. Fractions containing the symbiosome membrane (SM) and material from the lumen of the symbiosome (peribacteroid space or PBS) were isolated from soybean root nodules and analyzed using nongel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to reduce complexity of the samples and enrich for hydrophobic integral membrane proteins. One hundred and ninety-seven proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and PBS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and degradation, membrane trafficking, and solute transport were identified. These included a number of proteins previously localized to the SM, such as aquaglyceroporin nodulin 26, sulfate transporters, remorin, and Rab7 homologs. Among the proteome were a number of putative transporters for compounds such as sulfate, calcium, hydrogen ions, peptide/ dicarboxylate, and nitrate, as well as transporters for which the substrate is not easy to predict. Analysis of the promoter activity for six genes encoding putative SM proteins showed nodule specific expression, with five showing expression only in infected cells. Localization of two proteins was confirmed using GFP-fusion experiments. The data have been deposited to the ProteomeXchange with identifier PXD001132. This proteome will provide a rich resource for the study of the legumerhizobium symbiosis. Molecular & Cellular Proteomics

show abstract

“…Metabolic flux studies with developing seeds have added a quantitative understanding of in vivo pathway usage in oilseeds (O'Grady et al, 2012). To modulate seed oil levels or composition, various attempts have been chosen, mostly relying on introducing specific enzymes (Weselake et al, 2008;Kelly et al, 2013) or metabolite transporters (Fuchs et al, 2013;Kim et al, 2013). A number of seed-specific transcription factors are known to affect oil storage capabilities (Le et al, 2010); their modulated expression as well as the introduction of engineered transcription factors can enable a more global modulation of oilseed metabolism (Century et al, 2008;Gupta et al, 2012;Singh et al, 2013).…”

mentioning

confidence: 99%

Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture

et al. 2015

View full text Add to dashboard Cite

Seeds provide the basis for many food, feed, and fuel products. Continued increases in seed yield, composition, and quality require an improved understanding of how the developing seed converts carbon and nitrogen supplies into storage. Current knowledge of this process is often based on the premise that transcriptional regulation directly translates via enzyme concentration into flux. In an attempt to highlight metabolic control, we explore genotypic differences in carbon partitioning for in vitro cultured developing embryos of oilseed rape (Brassica napus). We determined biomass composition as well as 79 net fluxes, the levels of 77 metabolites, and 26 enzyme activities with specific focus on central metabolism in nine selected germplasm accessions. Overall, we observed a tradeoff between the biomass component fractions of lipid and starch. With increasing lipid content over the spectrum of genotypes, plastidic fatty acid synthesis and glycolytic flux increased concomitantly, while glycolytic intermediates decreased. The lipid/starch tradeoff was not reflected at the proteome level, pointing to the significance of (posttranslational) metabolic control. Enzyme activity/flux and metabolite/flux correlations suggest that plastidic pyruvate kinase exerts flux control and that the lipid/starch tradeoff is most likely mediated by allosteric feedback regulation of phosphofructokinase and ADP-glucose pyrophosphorylase. Quantitative data were also used to calculate in vivo mass action ratios, reaction equilibria, and metabolite turnover times. Compounds like cyclic 39,59-AMP and sucrose-6-phosphate were identified to potentially be involved in so far unknown mechanisms of metabolic control. This study provides a rich source of quantitative data for those studying central metabolism.Seeds develop by absorbing nutrients from their mother plant and using these to synthesize a combination of starch, protein, and lipid. The size and number of seeds that finally develop determine the crop's yield, while their composition determines the end-use quality of the crop. The conversion of nutrients into storage products involves a complex network of metabolic reactions, many of which are subject to transcriptional, translational, and posttranslational regulation. Attempting to engineer seed composition clearly requires a firm understanding of these regulatory networks.The seed's central metabolism differs markedly from those of both a photosynthesizing leaf and a root. In most species, the immature seed is green for a period during its development, so during this phase it is regarded as being photoheterotrophic. A further level of complexity arises as a result of spatial heterogeneity within the seed (Rolletschek et al., 2011; Borisjuk et al.,

show abstract

AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum

Cited by 113 publications

References 33 publications

Transport and transcriptional regulation of oil production in plants

Transport and transcriptional regulation of oil production in plants

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Quantitative Multilevel Analysis of Central Metabolism in Developing Oilseeds of Oilseed Rape during in Vitro Culture

Contact Info

Product

Resources

About