Atividades antimicrobiana e leishmanicida das sementes de Pterodon emarginatus Vogel

Dutra, Rafael C.; Braga, Fernanda Gambogi; Coimbra, Elaine Soares; Silva, Adilson D.; Barbosa, Nádia R.

doi:10.1590/s0102-695x2009000300016

Cited by 31 publications

(20 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results were described by Dutra et al .,[15] when evaluating the MIC of ethanolic of P. emarginatus using broth diffusion methods against S. aureus . The authors observed that, in the broth microdilution test, the extract presented antibacterial activity with MIC at 2.5 mg/mL.…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Inhibitory effects of Pterodon emarginatus bean oil and extract on Staphylococcus aureus

et al. 2017

View full text Add to dashboard Cite

Background:Pterodon emarginatus is a tree of the Brazilian Savannah. The beans of this tree are used in folk medicine as anti-inflammatory preparations, especially for infections caused by Staphylococcus aureus. These bacteria can cause simple infections or serious illnesses such as pneumonia, meningitis, endocarditis, toxic shock syndrome, septicemia, and others.Objective:This study had the goal of verifying the effect of the essential oil (OE) from P. emarginatus on the inhibition of S. aureus in culture medium, i.e., “ in vitro” tests.Materials and Methods:The vegetable material was cut and crushed with a press. The OE was obtained by extraction using hexane, alcohol, and water. The P. emarginatus extracts obtained were used to evaluate the antimicrobial effect on S. aureus (ATCC 25923) by tests of well diffusion, disc diffusion, and microdilution. The strain used in the assays was maintained in brain heart infusion broth and nutrient agar until testing. Afterward, the bacteria were spread on agar plates with Mueller-Hinton agar medium. In the wells and on the paper discs, the OE suspensions were placed in the following volumes: 10, 15, 20, 25, 30, 40, and 80 μL and subsequently they were incubated at 35°C ± 2°C. After 24 h, the number of colony-forming unit was determined.Results:Pure OE and hydroalcoholic extract inhibited the growth of S. aureus, while aqueous extract had no effect on bacterial growth in all microbial methods used.Conclusion:Thus, the present study showed the potential of sucupira-based extracts against S. aureus growth, opening new perspectives for the evaluation of these bioactive compounds as phytopharmaceutical products.SUMMARY Plant extract act as antimicrobials to prevent and reduce bacterial contaminationBeans of Pterodon emarginatus has antibacterial propertiesExtraction with different solvents might implicate on the rate of bacterial deathThe effect of different microbiological methods (well diffusion, disc diffusion and microdilution) was evaluated on reducing CFUThe results showed by MBC that concentrations superior to 10% (v/v) using AC and 7.5% (v/v) using OE were necessary to eliminate colonies formedAccording to data of MIC, at 2.5% of AC and OE was enough to kill S. aureusThe well diffusion technique demonstrated better performance than disc diffusion test for OE and AC extractsHydroalcoholic and oil extracts of sucupira beans had highest effect against Staphylococcus aureusAqueous extract had no effect on bacterial growth in all microbial methods testedThe sucupira-based extracts is a promising source as herbal drug due to therapeutic value Abbreviations Used: OE: Essencial oil; AC: Hydroalcoholic oil extract; AQ: Aqueous extracts; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony formed unit.

show abstract

Section: Discussionsupporting

confidence: 86%

“…[15] In animals, S. aureus can contaminate and produce various abscesses and pyogenic infections such as endocarditis, septic arthritis and osteomyelitis, food poisoning syndrome, desquamation, and toxic shock syndrome. [1617] Microbial resistance to various drugs is increasing.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of Pterodon emarginatus bean oil and extract on Staphylococcus aureus

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In fact, previous reports indicate save treatment with P. pudescens extracts components without side effects observed (Dutra et al, 2009).…”

Section: Parasite Per Macrophage Cells Viability (%)mentioning

confidence: 95%

“…Several works suggest immuno modulatory and anti-protozoa effect of plant component of Pterodon genera without side effects observed (Cardoso et al, 2008;Menna-Barreto et al, 2008;Dutra et al, 2009;Coelho et al, 2004). Recently, anti-tumoral effects have been shown for Pterodon pubescens extracts using in vitro model of leukemia (Pereira et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Preliminary phytochemical and antileishmanial studies of the ethanolic extracts of Pterodon pudescens

Arrais-Silva

Nunes

Carvalho

et al. 2014

Rev. bras. plantas med.

View full text Add to dashboard Cite

Pentavalent antimonials are the first choice for the treatment of human leishmaniasis. However in rural areas the traditional plants may be preferred for the treatment of lesions. In recent years a number of papers are published related to the natural products especially plant derivative with infectious diseases. The present work was undertaken to evaluate the antileishmanial activity of Pterodon pubescens which is a native tree widely distributed over the central region of Brazil and used in folk medicine as wine infusions to treat inflammatory disease. The phytochemical screening and the biological essay of ethanolic extract of Pterodon pudescens (PPE) leaves at the concentrations of 150, 300, 450, 600 μg/ml were tested in vitro in Leishmania amazonensis-infected macrophages to support its traditional medicinal use as a leishmaniasis remedy. Phytochemical screening of PPE has shown the presence of catechemical tannins, steroids, triterpenoids and flavonoids. The biological test suggests that PPE were found to control parasite burden of cell cultures in dose-dependent manner. These findings highlight the fact that the apparent potency of Pterodon pudescens compounds, together with their widely distribution over Latin America and Brazil, may represent a promising antileishmanial agent. Keywords: Pterodon pubscens, Leishmania amazonensis, Plant extractRESUMO: Estudos preliminares sobre a fitoquimica e a atividade anti-leishmania de extratos etanólicos de Pterodon pudescens. Antimoniais pentavalentes são a primeira escolha para o tratamento das leishmanioses humanas. No entanto, no interior brasileiro plantas tradicionais são usadas para o tratamento dessas lesões. De fato, recentes trabalhos tem relatado o potencial terapêutico de produtos naturais, especialmente derivados de plantas. Neste estudo avaliamos a atividade leishmanicida de Pterodon pubescens, uma árvore nativa, distribuída pela região central brasileira e usada em infusões para tratamento de inflamações. Foi realizada a análise fitoquímica e o ensaio in vitro em macrófagos infectados com Leishmania amazonensis em concentrações de 150, 300, 450, 600 μg/ml do extrato etanólico de folhas de Pterodon pudescens (PPE) para comprovar o uso tradicional desta planta como terapia para as leishmanioses. Os testes fitoquímicos indicaram a presença de taninos catequímicos, flavonas, esteroides, triterpenoides, flavonoides e xantonas. Os ensaios biológicos revelaram que o PPE foi capaz de controlar a carga parasitária em macrófagos de maneira dose dependente. Estes resultados corroboram com o potencial terapêutico de compostos de Pterodon pudescens e, junto com sua ampla distribuição no Brasil, podem representar promissor agente leishmanicida. Palavras-chaves: Pterodon pubscens, Leishmania amazonensis, Extrato de planta

show abstract

“…Although P. emarginatus is considered to be an important source of phytotherapeutic material because of its phytochemical characteristics and bioactive properties (Machado et al., ), few studies have been conducted on the use of its essential oil (Alves et al., ; Dutra et al., ; Mors et al., ; Pascoa et al., ; Santos et al., ). Because of the characteristics and properties of P. emarginatus , this plant has been included in the list of species that are a conservation priority (Alves et al., ).…”

Section: Introductionmentioning

confidence: 99%

Nanoemulsion from essential oil of Pterodon emarginatus (Fabaceae) shows in vitro efficacy against monogeneans of Colossoma macropomum (Pisces: Serrasalmidae)

Valentim

Duarte

Oliveira

et al. 2017

Journal of Fish Diseases

View full text Add to dashboard Cite

Outbreaks of diseases pose a major threat to sustainable aquaculture development worldwide. Application of herbal products to combat parasitic diseases provides an alternative approach for sustainable aquaculture. This study investigated the in vitro antiparasitic effects of an oil-in-water nanoemulsion prepared using the essential oil from Pterodon emarginatus, against monogeneans infesting Colossoma macropomum. Gill arches from C. macropomum (47.6 ± 14.5 g and 13.5 ± 1.4 cm) that were naturally parasitized by Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri were immersed in different dispersions of the P. emarginatus nanoemulsions (0, 50, 100, 200, 400 and 600 mg/L). The major compounds presented in the essential oil of P. emarginatus were β-elemene, β-caryophyllene and α-humulene. Characterization of these nanoemulsions showed that they have a small mean droplet size and low polydispersity index, which is concordant with stable systems. In this in vitro trial, the P. emarginatus nanoemulsion concentrations of 100, 200, 400 and 600 mg/L presented 100% helminthic efficacy against monogeneans of the gills of C. macropomum. The highest two concentrations used (400 and 600 mg/L) were seen to immobilize the parasites after only 15 min. Therefore, it would be worthwhile testing these concentrations in therapeutic baths against monogeneans of C. macropomum.

show abstract

Atividades antimicrobiana e leishmanicida das sementes de Pterodon emarginatus Vogel

Cited by 31 publications

References 44 publications

Inhibitory effects of Pterodon emarginatus bean oil and extract on Staphylococcus aureus

Inhibitory effects of Pterodon emarginatus bean oil and extract on Staphylococcus aureus

Preliminary phytochemical and antileishmanial studies of the ethanolic extracts of Pterodon pudescens

Nanoemulsion from essential oil of Pterodon emarginatus (Fabaceae) shows in vitro efficacy against monogeneans of Colossoma macropomum (Pisces: Serrasalmidae)

Contact Info

Product

Resources

About