Okadaic acid (OA) causes meiotic progression and chromosome condensation in cultured pachytene spermatocytes and an increase in maturation promoting factor (cyclin B1/cdc2 kinase) activity, as evaluated by H1 phosphorylative activity in anti-cyclin B1 immunoprecipitates. OA also induces a strong increase of phosphorylative activity toward the mitogen-activated protein kinase substrate myelin basic protein (MBP). Immunoprecipitation experiments with anti-extracellular signal-regulated kinase 1 (ERK1) or anti-ERK2 antibodies followed by MBP kinase assays, and direct in-gel kinase assays for MBP, show that p44/ERK1 but not p42/ERK2 is stimulated in OA-treated spermatocytes. OA treatment stimulates phosphorylation of ERK1, but not of ERK2, on a tyrosine residue involved in activation of the enzyme. ERK1 immunoprecipitated from extracts of OAstimulated spermatocytes induces a stimulation of H1 kinase activity in extracts from control pachytene spermatocytes, whereas immunoprecipitated ERK2 is uneffective. We also show that natural G 2 /M transition in spermatocytes is associated to intracellular redistribution of ERKs, and their association with microtubules of the metaphase spindle. Preincubation of cultured pachytene spermatocytes with PD98059 (a selective inhibitor of ERK-activating kinases MEK1/2) completely blocks the ability of OA to induce chromosome condensation and progression to meiotic metaphases. These results suggest that ERK1 is specifically activated during G 2 /M transition in mouse spermatocytes, that it contributes to the mechanisms of maturation promoting factor activation, and that it is essential for chromosome condensation associated with progression to meiotic metaphases.Male meiosis is a process in which a dyploid spermatocyte gives rise to four haploid round spermatids after a single round of DNA replication followed by two subsequent cell divisions. The homologous chromosomes are segregated in the two daughter cells (secondary spermatocytes) during the first meiotic division, whereas the sister chromatids, through a process resembling mitosis, are segregated during the second division to generate haploid cells. The prophase of male first meiotic division is a lengthy process which ensures the correct pairing and the crossing over between homologous chromosomes. In the mouse, this process lasts approximately 10 days, during which the homologous chromosomes become partially condensed, anneal to each other, and are maintained in proximity by a structure called the synaptonemal complex. After genetic exchange (crossing over) has occurred, the synaptonemal complex disappears and chromosome condensation allows the sites of DNA exchange to become visible as chiasmata. As for mitotic division, the nuclear envelope breaks down and microtubules are assembled into a spindle. The chromosomes become aligned to the equator of the meiotic spindle and the two sets of homologous chromosomes are separated during the anaphase. While the meiotic prophase is considerably long, metaphase and anaphase transitions ...