Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity

Hanbal, Sara E.; Takashima, Keisuke; Miyashita, Shuhei; Ando, Sugihiro; Ito, Kumiko; Elsharkawy, Mohsen Mohamed; Kaneko, Toshiro; Takahashi, Hideki

doi:10.1007/s00705-018-3909-4

Cited by 30 publications

(20 citation statements)

References 22 publications

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“…CP is produced by applying an electrical charge to a gas, which ionizes the gas to produce a mixture of excited molecules, charged particles, reactive oxygen and nitrogen species, and some UV light and ozone. As such it has unique antimicrobial properties that have been demonstrated to be effective against plant pathogenic bacteria (Moreau et al , ), and also tobacco mosaic virus (Hanbal et al , ). CP studies on common postharvest fungi such as Aspergillus parasiticus , A. oryzae and Penicillium digitatum have reported optimistic results, with up to 100% control following CP treatment of contaminated nuts (hazelnut, peanut and pistachio; Basaran et al , ), grains (wheat, barley and rice; Selcuk et al , ; Hayashi et al , ) and fruit (lemon and mandarin; Hayashi et al , ; Won et al , ).…”

Section: Introductionmentioning

confidence: 99%

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

2019

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This study investigated the use of cold plasma to reduce the in vitro growth of two postharvest fungal plant pathogens, Colletotrichum alienum and C. fioriniae, isolated from avocados. Cold plasma (CP) was used to treat pure cultures and conidial suspensions of both pathogens, for 180 or 360 s, in either open or sealed environments from varying distances. In an open environment, the 360 s treatment at a distance of 5 cm reduced the colony growth of freshly inoculated cultures to less than 2 mm/day compared to the control of more than 8 mm/day, and treatment of conidial suspensions resulted in almost 100% reduction of conidial germination. In the same environment, the 180 s CP treatment did not significantly reduce the colony growth of fresh or actively growing cultures, but did suppress the germination of conidia by up to 80%. In a sealed environment, the 360 s CP treatment also effectively reduced the growth of freshly inoculated cultures, with no growth for some isolates. Production of reactive oxygen and nitrogen species was observed during treatment, and these may have contributed to the reduction in growth and germination. These results demonstrate the potential of CP for the control of two Colletotrichum species.

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Section: Introductionmentioning

confidence: 99%

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

2019

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“…The antiviral effects of plasma have been occasionally demonstrated in human and animal viruses, as well as bacteriophages (Zimmermann et al, 2011;Aboubakr et al, 2015;Guo et al, 2018). The inactivation of plant viruses by plasma was demonstrated in a recent study (Hanbal et al, 2018). In this study, inoculation with plasma irradiated tobacco mosaic virus (TMV) solution did not induce the development of disease in tobacco plants, whereas necrotic local lesions developed on tobacco leaves inoculated with non-irradiated TMV solution (Hanbal et al, 2018).…”

Section: Viral Pathogensmentioning

confidence: 66%

“…The inactivation of plant viruses by plasma was demonstrated in a recent study (Hanbal et al, 2018). In this study, inoculation with plasma irradiated tobacco mosaic virus (TMV) solution did not induce the development of disease in tobacco plants, whereas necrotic local lesions developed on tobacco leaves inoculated with non-irradiated TMV solution (Hanbal et al, 2018). Min et al (2016) also showed that viral load was significantly reduced when romaine lettuce inoculated with Tulane virus was treated with DBD plasma.…”

Section: Viral Pathogensmentioning

confidence: 68%

Plant Disease Control by Non-Thermal Atmospheric-Pressure Plasma

et al. 2020

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Disease stresses caused by pathogenic microorganisms are increasing, probably because of global warming. Conventional technologies for plant disease control have often revealed their limitations in efficiency, environmental safety, and economic costs. There is high demand for improvements in efficiency and safety. Non-thermal atmospheric-pressure plasma has demonstrated its potential as an alternative tool for efficient and environmentally safe control of plant pathogenic microorganisms in many studies, which are overviewed in this review. Efficient inactivation of phytopathogenic bacterial and fungal cells by various plasma sources under laboratory conditions has been frequently reported. In addition, plasma-treated water shows antimicrobial activity. Plasma and plasma-treated water exhibit a broad spectrum of efficiency in the decontamination and disinfection of plants, fruits, and seeds, indicating that the outcomes of plasma treatment can be significantly influenced by the microenvironments between plasma and plant tissues, such as the surface structures and properties, antioxidant systems, and surface chemistry of plants. More intense studies are required on the efficiency of decontamination and disinfection and underlying mechanisms. Recently, the induction of plant tolerance or resistance to pathogens by plasma (so-called "plasma vaccination") is emerging as a new area of study, with active research ongoing in this field.

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“…none of these studies considered virus inactivation for water decontamination. Only two studies in the CAP-virus field have assessed the inactivation of viruses in larger volumes of water (Guo et al, 2018;Filipić et al, 2019), and only two have reported on the applications of CAP for inactivation of plant viruses, including potato virus Y (PVY; Filipić et al, 2019) and tomato mosaic virus (Hanbal et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Pepper Mild Mottle Virus in Water by Cold Atmospheric Plasma

et al. 2021

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Water scarcity is one of the greatest threats for human survival and quality of life, and this is increasingly contributing to the risk of human, animal and plant infections due to waterborne viruses. Viruses are transmitted through polluted water, where they can survive and cause infections even at low concentrations. Plant viruses from the genus Tobamovirus are highly mechanically transmissible, and cause considerable damage to important crops, such as tomato. The release of infective tobamoviruses into environmental waters has been reported, with the consequent risk for arid regions, where these waters are used for irrigation. Virus inactivation in water is thus very important and cold atmospheric plasma (CAP) is emerging in this field as an efficient, safe, and sustainable alternative to classic waterborne virus inactivation methods. In the present study we evaluated CAP-mediated inactivation of pepper mild mottle virus (PMMoV) in water samples. PMMoV is a very resilient water-transmissible tobamovirus that can survive transit through the human digestive tract. The efficiency of PMMoV inactivation was characterized for infectivity and virion integrity, and at the genome level, using test plant infectivity assays, transmission electron microscopy, and molecular methods, respectively. Additionally, the safety of CAP treatment was determined by testing the cytotoxic and genotoxic properties of CAP-treated water on the HepG2 cell line. 5-min treatment with CAP was sufficient to inactivate PMMoV without introducing any cytotoxic or genotoxic effects in the in-vitro cell model system. These data on inactivation of such stable waterborne virus, PMMoV, will encourage further examination of CAP as an alternative for treatment of potable and irrigation waters, and even for other water sources, with emphasis on inactivation of various viruses including enteric viruses.

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Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity

Cited by 30 publications

References 22 publications

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

Plant Disease Control by Non-Thermal Atmospheric-Pressure Plasma

Inactivation of Pepper Mild Mottle Virus in Water by Cold Atmospheric Plasma

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