Atomic Force Acoustic Microscopy

Rabe, U.

doi:10.1007/3-540-27453-7_2

Cited by 119 publications

(164 citation statements)

References 106 publications

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“…Because of the detection of the acoustic amplitude signal which allows us to distinguish different levels of softness of surface layers, it is possible to evaluate the layer quality. 59 The printed stripes on a Si wafer 100 with native oxide ( Figure 3A) show a homogeneous layer and monolayer quality except for the edges of the stamp stripes were silane solution can concentrate during printing because of the applied pressure. Therefore, careful drying of the incubated stamps is necessary for homogeneous monolayers.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Patterned Biochemical Functionalization Improves Aptamer-Based Detection of Unlabeled Thrombin in a Sandwich Assay

Römhildt

Pahlke²,

Zörgiebel³

et al. 2013

ACS Appl. Mater. Interfaces

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Here we propose a platform for the detection of unlabeled human α-thrombin down to the picomolar range in a fluorescence-based aptamer assay. In this concept, thrombin is captured between two different thrombin binding aptamers, TBA1 (15mer) and TBA2 (29mer), each labeled with a specific fluorescent dye. One aptamer is attached to the surface, the second one is in solution and recognizes surface-captured thrombin. To improve the limit of detection and the comparability of measurements, we employed and compared two approaches to pattern the chip substrate−microcontact printing of organosilanes onto bare glass slides, and controlled printing of the capture aptamer TBA1 in arrays onto functionalized glass substrates using a nanoplotter device. The parallel presence of functionalized and control areas acts as an internal reference. We demonstrate that both techniques enable the detection of thrombin concentrations in a wide range from 0.02 to 200 nM with a detection limit at 20 pM. Finally, the developed method could be transferred to any substrate to probe different targets that have two distinct possible receptors without the need for direct target labeling.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Patterned Biochemical Functionalization Improves Aptamer-Based Detection of Unlabeled Thrombin in a Sandwich Assay

Römhildt

Pahlke²,

Zörgiebel³

et al. 2013

ACS Appl. Mater. Interfaces

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show abstract

“…A variety of different modeling approaches have been used for this purpose and have been applied with a great deal of success to both hard materials and to soft materials that exhibit viscoelastic behavior. 23,29 In this work, we confine ourselves to demonstrating the utility of electrostatic actuation for measuring contact resonance frequencies, without emphasizing any particular modeling approach for extracting quantitative mechanical property information from the contact resonance observables.…”

Section: Contact Resonance Microscopymentioning

confidence: 99%

Modular apparatus for electrostatic actuation of common atomic force microscope cantilevers

Long

Cannara

2015

Review of Scientific Instruments

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Piezoelectric actuation of atomic force microscope (AFM) cantilevers often suffers from spurious mechanical resonances in the loop between the signal driving the cantilever and the actual tip motion. These spurious resonances can reduce the accuracy of AFM measurements and in some cases completely obscure the cantilever response. To address these limitations, we developed a specialized AFM cantilever holder for electrostatic actuation of AFM cantilevers. The holder contains electrical contacts for the AFM cantilever chip, as well as an electrode (or electrodes) that may be precisely positioned with respect to the back of the cantilever. By controlling the voltages on the AFM cantilever and the actuation electrode(s), an electrostatic force is applied directly to the cantilever, providing a near-ideal transfer function from drive signal to tip motion. We demonstrate both static and dynamic actuations, achieved through the application of direct current and alternating current voltage schemes, respectively. As an example application, we explore contact resonance atomic force microscopy, which is a technique for measuring the mechanical properties of surfaces on the sub-micron length scale. Using multiple electrodes, we also show that the torsional resonances of the AFM cantilever may be excited electrostatically, opening the door for advanced dynamic lateral force measurements with improved accuracy and precision.

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“…This technique is based on transmission and reflection of ultrasonic waves [Rabe, 2006]. The acoustic microscope is a confocal system, this is that focus occurs when both acoustic waves travel through the specimen and are detected by the lenses.…”

Section: Atomic Force Acoustic Microscopy (Afam)mentioning

confidence: 99%

“…Depending on the local contact stiffness the resonance frequency will change and consequently the vibration amplitude of the work frequency will change, which will also be reflected in contrast differences in amplitude images. These images provide qualitative information about differences in stiffness in regions of the sample surface [KopycinskaMüller, et al, 2007;Rabe, 2006].…”

Section: Atomic Force Acoustic Microscopy (Afam)mentioning

confidence: 99%

“…Amplitude vibration of the surface is mapped by a cantilever (excited at a fixed frequency near its resonance). Depending on the variations of the local contact stiffness, resonance frequency will vary, causing the amplitude vibration of the work frequency to change, which will be reflected in contrast differences on the amplitude images [Rabe, 2006;Kopycinska-Müller et al, 2007]. In this chapter, the elastic and viscoelastic behaviour, hardness and nanowear characterization of SiO 2 -PMMA hybrid coatings by atomic force acoustic microscopy (AFAM) imaging and nanoinentation are presented and discussed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Elastic and Nanowearing Properties of SiO2-PMMA and Hybrid Coatings Evaluated by Atomic Force Acoustic Microscopy and Nanoindentation"

Alvarado-Rivera¹,

Muñoz-Saldaña²,

Ramı́rez-Bon³

2012

Scanning Probe Microscopy-Physical Property Characterization at Nanoscale

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Atomic Force Acoustic Microscopy

Cited by 119 publications

References 106 publications

Patterned Biochemical Functionalization Improves Aptamer-Based Detection of Unlabeled Thrombin in a Sandwich Assay

Patterned Biochemical Functionalization Improves Aptamer-Based Detection of Unlabeled Thrombin in a Sandwich Assay

Modular apparatus for electrostatic actuation of common atomic force microscope cantilevers

Elastic and Nanowearing Properties of SiO2-PMMA and Hybrid Coatings Evaluated by Atomic Force Acoustic Microscopy and Nanoindentation"

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