Atomic force microscopy study of the antibacterial effect of fosfomycin on methicillin-resistant Staphylococcus pseudintermedius

Abstract

The influence of fosfomycin on methicillinresistant Staphylococcus pseudintermedius (MRSP) as the target cell was studied by atomic force microscopy (AFM). Nanoscale images of the effects of the antibiotic fosfomycin on this gram-positive bacterium's cell were obtained in situ without fixing agents. Our study has demonstrated substantial morphological and topographical differences between the control and fosfomycin-treated MRSP cells. The AFM investigations further revealed the rough surface morphology and a 30 % shrinkage in size of the fosfomycin-treated cell and the leakage of cytoplasmic components from the cell. The damage of cell membrane integrity and the cell surface degradation as observed elaborates the antibacterial activity of fosfomycin. The AFM image analysis also reveals that the fosfomycin inhibits cell division, and prevents the adhesion on the surface discouraging the biofilm attachment. The microtitre plate assay results conform to the atomic force microscopy image analysis. This is the first visual demonstration of the effect of fosfomycin on MRSP cells.

Antiplanktonic, antibiofilm, antiswarming motility and antiquorum sensing activities of green synthesized Ag–TiO2, TiO2–Ag, Ag–Cu and Cu–Ag nanocomposites against multi-drug-resistant bacteria

“…Hereon, the most reliable conclusion of these results is interaction increment of NCs with cell membrane compared with control. Similarly, treatment of Staphylococcus pseudintermedius by cell wall disturbing antibiotic (fosfomycin) illustrated synchronously cell shape changing with increasing of roughness than control samples [42]. In contrast to this study, biofilm roughness of Acinetobacter baumannii, E. coli, and S. aureus strains decreased than that of control sample, which may be due to the difference in surface type [43] because, in this study, silicon surface was applied to survey biofilm formation instead of glass slide.…”

New Genus Fibralongavirus in Siphoviridae Phages of Staphylococcus pseudintermedius

“…With internal chaperon domain, there is probably no need for next tail fibre assembly protein as is the case with many other phages [73]. This long tail fibre might play a role in fast adsorption that was observed even on a phage resistant strain, or its length is required to attach to a membrane receptor under thick S. pseudintermedius cell wall [74]. Long tail fibres were described in Bacillus viruses γ and Wβ, Escherichia virus T5, Salmonella virus χ, and similar phages.…”

Synthesis and fabrication of high‐potent antimicrobial polymeric ultrathin coatings

“…Ruptures or depressions or holes distributed on cell surface demonstrate the shape alteration induced by the polymer. 9,[51][52][53] Stability Test for Ultrathin Coating The stability of ultrathin coating was investigated in two different pH values. The polymer ultrathin coating was stable for longterm usage.…”