Atomic Force Microscopy Study of Early Morphological Changes during Apoptosis

Hessler, Jessica A.; Budor, Andrew; Putchakayala, Krishna; Mecke, Almut; Rieger, Daniel; Holl, Mark M. Banaszak; Orr, Bradford G.; Bielinska, Anna U.; Beals, John M.; Baker, James R.

doi:10.1021/la051837g

Cited by 92 publications

(77 citation statements)

References 29 publications

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“…Recently, in a study of early morphological changes during staurosporine-induced apoptosis using atomic force microscopy in human epidermoid carcinoma (KB) cells, AVD was shown to precede key biochemical hallmarks of apoptosis including loss of the mitochondrial membrane potential, externalization of membrane phosphatidylserine, nuclear fragmentation, and measurable caspase-3 activity [26]. Additionally, it has been shown that AVD once initiated occurs within 12 to 20 minutes depending on the cell type and stimulus studied [27;Bortner and Cidlowski,unpublished data].…”

Section: Potassium As a Key Player In The Regulation Of Apoptosismentioning

confidence: 99%

Cell shrinkage and monovalent cation fluxes: Role in apoptosis

Bortner

Cidlowski

2007

Archives of Biochemistry and Biophysics

230

193

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The loss of cell volume or cell shrinkage has been a morphological hallmark of the programmed cell death process known as apoptosis. This isotonic loss of cell volume has recently been term apoptotic volume decrease or AVD to distinguish it from inherent volume regulatory responses that occurs in cells under anisotonic conditions. Recent studies examining the intracellular signaling pathways that result in this unique cellular characteristic have determined that a fundamental movement of ions, particularly monovalent ions, underlie the AVD process and plays an important role on controlling the cell death process. An efflux of intracellular potassium was shown to be a critical aspect of the AVD process, as preventing this ion loss could protect cells from apoptosis. However, potassium plays a complex role as a loss of intracellular potassium has also been shown to be beneficial to the health of the cell. Additionally, the mechanisms that a cell employs to achieve this loss of intracellular potassium vary depending on the cell type and stimulus used to induce apoptosis, suggesting multiple ways exist to accomplish the same goal of AVD. Additionally, sodium and chloride have been shown to play a vital role during cell death in both the signaling and control of AVD in various apoptotic model systems. This review examines the relationship between this morphological change and intracellular monovalent ions during apoptosis.

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Section: Potassium As a Key Player In The Regulation Of Apoptosismentioning

confidence: 99%

Cell shrinkage and monovalent cation fluxes: Role in apoptosis

Bortner

Cidlowski

2007

Archives of Biochemistry and Biophysics

230

193

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“…[18][19][20][21] Previous study indicated that the cell morphology 22,23 and the cytoskeletal filaments 24 would affect the mechanical properties. To gain a comprehensive picture of the structures of hAFSCs and differentiated osteoblasts, AFM was utilized to obtain high-resolution three-dimensional morphology (Fig.…”

Section: Afm Image Of the Hafscs And Differentiated Osteoblastsmentioning

confidence: 99%

AFM Studies of Cellular Mechanics during Osteogenic Differentiation of Human Amniotic Fluid-derived Stem Cells

et al. 2010

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“…The decreased fluorescence intensity of FMSNs after 24 h incubation may be due to the apoptosis-induced increase in cell membrane permeability and the accelerated nanoparticle diffusion. 36 Furthermore, MTT tests were conducted to elucidate the therapeutic potential of the nanovehicles. A cancer cell growth inhibitor effect was observed in HeLa cells and NIH-3T3 cells after treatment with FHMSNs@GNC@HA, Dox-FHMSNs-Pep1, Dox-FHMSNs@GNC or Dox-FHMSNs@GNC@HA.…”

Section: Controlled Drug Release Within the Cells And Selective Toxicitymentioning

confidence: 99%

Peptide-mediated core/satellite/shell multifunctional nanovehicles for precise imaging of cathepsin B activity and dual-enzyme controlled drug release

Zheng

Zhang

et al. 2017

NPG Asia Mater

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Intracellular imaging of pathologically relevant proteases can provide essential information for the accurate evaluation of disease stage and progression. However, the risks of degradation by nonspecific enzymes during transportation and poor cellular uptake limit the use of peptide-based molecular probes (PMPs) for in situ protease imaging. To overcome these obstacles, a self-protected nanovehicle with a peptide-mediated core/satellite/shell structure was constructed for precise imaging of the protease cathepsin B (Cat B) in situ and the subsequent Cat B-responsive drug release. The self-protected nanovehicles demonstrated excellent resistance to nonspecific enzymolysis, thereby keeping the embedded PMPs intact until they reach the target organelle. Furthermore, the targeting ability of the outer shell facilitated the internalization of nanovehicles into tumor cells via receptor-guided recognition: the protective shell thereafter degraded in the intracellular microenvironment, and Cat B activity was dynamically monitored as the core/satellite structure disassembled. Meanwhile, the peptide-mediated satellite/shell structures served as three-dimensional gatekeepers to lock doxorubicin inside the nanovehicles, and drug release was spatiotemporally controlled by Cat B activity. This study provides important guiding principles for the rational design of self-protected nanovehicles for accurate diagnosis and therapy.

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Atomic Force Microscopy Study of Early Morphological Changes during Apoptosis

Cited by 92 publications

References 29 publications

Cell shrinkage and monovalent cation fluxes: Role in apoptosis

Cell shrinkage and monovalent cation fluxes: Role in apoptosis

AFM Studies of Cellular Mechanics during Osteogenic Differentiation of Human Amniotic Fluid-derived Stem Cells

Peptide-mediated core/satellite/shell multifunctional nanovehicles for precise imaging of cathepsin B activity and dual-enzyme controlled drug release

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