Atomic view of the histidine environment stabilizing higher-pH conformations of pH-dependent proteins

Valéry, Céline; Deville-Foillard, Stéphanie; Lefebvre, Christelle; Taberner, Nuria; Legrand, Pierre; Meneau, Florian; Mériadec, Cristelle; Delvaux, Camille; Bizien, Thomas; Kasotakis, Emmanouil; López‐Iglesias, Carmen; Gall, Andrew; Bressanelli, Stéphane; Du, Marie-Hélène Le; Paternostre, Maı̈té; Artzner, Franck

doi:10.1038/ncomms8771

Cited by 39 publications

(42 citation statements)

References 41 publications

(53 reference statements)

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“…Conversely, at increasing pH-values, histidine loses one proton in its imidazole ring, which gradually gives rise to neutral forms ( Figure 9D). Valery et al (2015) studied the conformational change of self-assembled histidine-containing peptides and their stabilized globular conformation at high pH. Vibrational spectroscopy assessments revealed histidineserine H-bond and histidine-aromatic interactions.…”

Section: Interpretation Of the Raman Analysesmentioning

confidence: 99%

Activity and Mechanism of Action of the Bioceramic Silicon Nitride as an Environmentally Friendly Alternative for the Control of the Grapevine Downy Mildew Pathogen Plasmopara viticola

et al. 2020

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Downy mildew of grapevine, caused by Plasmopara viticola (Berk. and Curt.) Berl. and de Toni, is one of the most devastating diseases of grapevine, severely affecting grape and wine production and quality worldwide. Infections are usually controlled by the intensive application of synthetic fungicides or by copper-based products in organic farming, rising problems for soil contamination and adverse impacts on environment and human health. While strict regulations attempt to minimize their harmful consequences, the situation calls for the development of alternative fungicidal strategies. This study presents the unprecedented case of a bioceramic, silicon nitride, with antimicrobial properties against P. viticola, but without adverse effects on human cells and environment, opening the way to the possible extension of silicon nitride applications in agriculture. Raman spectroscopic assessments of treated sporangia in conjunction with microscopic observations mechanistically showed that the nitrogen-chemistry of the bioceramic surface affects pathogen’s biochemical components and cell viability, thus presenting a high potential for host protection from P. viticola infections.

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Section: Interpretation Of the Raman Analysesmentioning

confidence: 99%

Activity and Mechanism of Action of the Bioceramic Silicon Nitride as an Environmentally Friendly Alternative for the Control of the Grapevine Downy Mildew Pathogen Plasmopara viticola

et al. 2020

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“…The rational design and usage of defined amyloid‐based nanostructures are still being hindered by several issues, which include the high aggregation propensity of amyloidogenic β‐sheet‐forming sequences, the lack of control over the self‐assembly process and the difficulty of predicting the final supramolecular architecture from the primary sequence . In contrast to polymers built on covalent bonds, bottom‐up polypeptide amyloid self‐assembly relies on the formation of a complex and organized network of intermolecular noncovalent interactions .…”

Section: Introductionmentioning

confidence: 99%

Guiding the Morphology of Amyloid Assemblies by Electrostatic Capping: from Polymorphic Twisted Fibrils to Uniform Nanorods

Zottig

Al‐Halifa

Babych

et al. 2019

Small

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Peptides that self‐assemble into cross‐β‐sheet amyloid structures constitute promising building blocks to construct highly ordered proteinaceous materials and nanoparticles. Nevertheless, the intrinsic polymorphism of amyloids and the difficulty of controlling self‐assembly currently limit their usage. In this study, the effect of electrostatic interactions on the supramolecular organization of peptide assemblies is investigated to gain insights into the structural basis of the morphological diversities of amyloids. Different charged capping units are introduced at the N‐terminus of a potent β‐sheet‐forming sequence derived from the 20–29 segment of islet amyloid polypeptide, known to self‐assemble into polymorphic fibrils. By tuning the charge and the electrostatic strength, different mesoscopic morphologies are obtained, including nanorods, rope‐like fibrils, and twisted ribbons. Particularly, the addition of positive capping units leads to the formation of uniform rod‐like assemblies, with lengths that can be modulated by the charge number. It is proposed that electrostatic repulsions between N‐terminal positive charges hinder β‐sheet tape twisting, leading to a unique control over the size of these cytocompatible nanorods by protofilament growth frustration. This study reveals the high susceptibility of amyloid formation to subtle chemical modifications and opens to promising strategies to control the final architecture of proteinaceous assemblies from the peptide sequence.

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“…The second technique was based on a recent study of Valery et al ., which showed that a change of pH value and thus deprotonation of the His residue in the GnRH agonist, TRH, could trigger a self-assembly of the peptide into nanotubes 22 . This pH dependent switch can be applied in the bottom-up development of pH-responsive nanomaterials as well as for the in vitro induced conformational change and fibrillation of GnRH [6-D-Phe].…”

Section: Resultsmentioning

confidence: 99%

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils

Yordanova

Vanderlinden

Stoll

et al. 2018

Sci Rep

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A synthetic derivative, GnRH [6-D-Phe], stable against enzymatic degradation, self-assembles and forms nanostructures and fibrils upon a pH shift in the presence of different concentrations of Zn2+ in vitro. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR–FTIR) revealed the existence of higher order assembly of Zn2+: GnRH [6-D-Phe]. Nuclear Magnetic Resonance spectroscopy (NMR) indicated a weak interaction between Zn2+ and GnRH [6-D-Phe]. Atomic Force Microscopy (AFM) showed the existence of GnRH [6-D-Phe] oligomers and fibrils. Molecular Dynamic (MD) simulation of the 10:1 Zn2+: GnRH [6-D-Phe] explored the interaction and dimerization processes. In contrast to already existing short peptide fibrils, GnRH [6-D-Phe] nanostructures and fibrils form in a Tris-buffered pH environment in a controlled manner through a temperature reduction and a pH shift. The lyophilized Zn2+: GnRH [6-D-Phe] assembly was tested as a platform for the sustained delivery of GnRH [6-D-Phe] and incorporated into two different oil vehicle matrices. The in vitro release was slow and continuous over 14 days and not influenced by the oil matrix.

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Atomic view of the histidine environment stabilizing higher-pH conformations of pH-dependent proteins

Cited by 39 publications

References 41 publications

Activity and Mechanism of Action of the Bioceramic Silicon Nitride as an Environmentally Friendly Alternative for the Control of the Grapevine Downy Mildew Pathogen Plasmopara viticola

Activity and Mechanism of Action of the Bioceramic Silicon Nitride as an Environmentally Friendly Alternative for the Control of the Grapevine Downy Mildew Pathogen Plasmopara viticola

Guiding the Morphology of Amyloid Assemblies by Electrostatic Capping: from Polymorphic Twisted Fibrils to Uniform Nanorods

Zn2+-triggered self-assembly of Gonadorelin [6-D-Phe] to produce nanostructures and fibrils

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