Atopic NC/Nga Mice as a Model for Allergic Asthma: Severe Allergic Responses by Single Intranasal Challenge with Protein Antigen.

Abstract

ABSTRACT. Since certain characters of allergic asthma are common with other allergic disorders like atopic dermatitis, the possible relationship in etiology is expected. Herein, we investigated whether NC/Nga mice, an inherent animal model for human atopic dermatitis, are inclined to allergic asthma. A single intranasal challenge of NC/Nga mice immunized with ovalbumin (OVA) resulted in an increase in plasma levels of OVA-specific IgE, and typical pathological aspects of allergic asthma characterized by infiltration of numerous eosinophils, mucus hyper production of bronchial epithelial cells. Moreover, airway hyperresponsiveness to inhaled acetylcholine and marked enhancement of airway resistance after the challenge were observed as compared to control BALB/c mice. Delayed expression of mRNA of eosinophil active chemokines, interleukin-5, eotaxin, macrophage inflammatory protein-1α in concert with eosinophilia was determined in the lung of NC/Nga mice. These results suggest that asthmatic responses developed in NC/Nga mice challenged with OVA are very similar to human allergic asthma, and that NC/Nga mice are a useful model to elucidate various aspects of allergic asthma. KEY WORDS: allergy, cytokine, eosinophil, in vivo animal model, lung.

Oral Administration of Lactic Acid Bacteria Isolated from Traditional South Asian Fermented Milk `Dahi' Inhibits the Development of Atopic Dermatitis in NC/Nga Mice

“…The repetitive application of haptens such as PiCl and DNFB induces a Th2 immune response (26,27). The skin lesions, with increased numbers of eosinophils, mast cells, CD4-positive T cells, and macrophages, are histopathologically similar to those in human AD and develop with elevation of serum total IgE level in NC/Nga mice (28). In this study, we demonstrated that the oral administration of heat-treated R- 037 alleviated the development of AD-like skin lesions in NC/Nga mice.…”

Accelerated differentiation of bone marrow-derived dendritic cells in atopic prone mice

“…Previous studies have suggested potency of NC/Nga mice as a model of human allergic/atopic diseases including atopic dermatitis and bronchial asthma [20]. NC/Nga mice with experimentally induced asthma show severe eosinophilia as compared with BALB/c mice [21]. Our previous study has also shown that weekly intratracheal administration of diesel exhaust particles lead airway inflammation such as infiltration of neutrophils and mononuclear cells in NC/Nga mice [20], however, same experimental protocol does not influence lung inflammation in ICR mice [22].…”

Atopic NC/Nga Mice as a Model for Allergic asthma: Cytokine Profiles and Eosinophil Productivity of Bone Marrow.

“…IL-5 is well characterized as a differentiation factor of eosinophils [4,25,32], and tracheal application of this cytokine to guinea pig was shown to induce eosinophilic inflammation in lung [17]. In spite of the marked difference in the airway inflammation, which was shown in our previous study [14], we found no significant difference in their levels in BAL fluid between both NC/Nga mice and BALB/c mice (Fig. 1); both were rapidly increased after the challenge and peaked at 24 hr in both mice strains in comparable fashion.…”

“…In these models the NC/Nga mice showed severe eosinophilic inflammation and marked elevation of allergen specific IgE titer in plasma, which are known as common and major symptoms in allergic diseases. Although all these symptoms are intense in the NC/Nga mice than other mice strains in the asthmatic model, eosinophilic inflammation is remarkable because it is not only severe in scale, but also persistent [14].…”

“…Recently increasing evidence has suggested the potency of NC/Nga mice as models of human allergic diseases like atopic dermatitis, and bronchial asthma [14,17,18,29]. In these models the NC/Nga mice showed severe eosinophilic inflammation and marked elevation of allergen specific IgE titer in plasma, which are known as common and major symptoms in allergic diseases.…”