Atopy patch test with different vehicles and allergen concentrations: An approach to standardization☆☆☆★

Darsow, Ulf; Vieluf, D.; Ring, Johannes

doi:10.1016/s0091-6749(95)70172-9

Cited by 162 publications

(144 citation statements)

References 24 publications

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“…Commercial reagents are available for a few allergens (1185). They have been standardized regarding the use of vehicle and dose-response relationships (1186,1187). A subset of patients with atopic dermatitis show only atopy patch test positivity while specific IgE to the same allergen remains negative.…”

Section: Skin Testsmentioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,859

1,761

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Section: Skin Testsmentioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,859

1,761

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“…The atopy patch test (APT) 67 is about to be standardized for the search of AD-relevant allergen. While the sensitivity of APT is rather average, its specificity is high for the individual context of a given patient 68 . Most importantly, laboratory results have always to be interpreted in the context of the patient's history and skin tests.…”

Section: Diagnostic Of Atopic Dermatitismentioning

confidence: 99%

Atopic Dermatitis

2010

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“…house dust mites, weeds, animal dander and moulds) occurs in a certain proportion of AE patients that are sensitized to such allergens. However, the reaction is usually negative in normal healthy volunteers and in patients with pure respiratory allergy [8, 23, 24]. We therefore used the patch test as the method of antigen challenge.…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of RANTES, CCR3 and CCR5 in the Lesional Skin of Patients with Atopic Eczema

Kato

Pawankar

Kimura

et al. 2006

Int Arch Allergy Immunol

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Background: Atopic eczema (AE) is a relapsing inflammatory disease based on IgE sensitization and characterized by peripheral blood eosinophilia and eosinophil infiltration into the lesional skin. In the patch test reaction of AE by allergens, an increased infiltration of activated eosinophils has been demonstrated peaking at 24–48 h. Regulated on activation normal T cell expressed and secreted (RANTES/CCL5) is a chemokine that induces eosinophil migration, and CCR3 and CCR5 are the receptors of RANTES. Objective: In order to further clarify the pathomechanisms of eosinophil infiltration in ongoing chronic inflammation in the skin of patients with AE and its relation to disease severity, we examined the expression of RANTES and its receptors CCR3 and CCR5 in challenged and unchallenged lesional skin of AE. Methods: We examined the number of RANTES+ cells, CCR3+ cells, CCR5+cells, activated (EG2+) eosinophils and CD3+ T cells in normal skin of healthy volunteers, and in challenged lesional skin (24 h after mite patch test) as well as unchallenged lesional skin of AE patients by immunohistochemistry. The cellular source of RANTES, CCR3 and CCR5 was analyzed by double immunohistochemistry using specific antibodies to RANTES, CCR3 or CCR5, and antibodies to ECP (EG2) or CD3. Results: The numbers of RANTES+ cells, CCR3+ cells, CCR5+ cells, EG2+ cells and CD3+ cells were all significantly increased in challenged (mite patch-tested) lesional skin of AE patients as compared to those in unchallenged lesional skin and normal skin. The numbers of these cells in unchallenged lesional skin were greater than those in normal skin. The number of EG2+ cells in the unchallenged lesional skin correlated with both the peripheral blood eosinophil count and the SCORAD index. The number of EG2+ cells in challenged lesional skin correlated with the number of CCR5+ cells. Activated eosinophils and T cells expressed RANTES and various proportions of these cells were CCR3+ and CCR5+ in both challenged and unchallenged lesional skin. Conclusion: Taken together, these results suggest that RANTES as well as its receptors CCR3 and CCR5 may play important roles in the orchestration of eosinophil infiltration in ongoing chronic inflammation in AE, and also reflect the severity of disease.

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Atopy patch test with different vehicles and allergen concentrations: An approach to standardization☆☆☆★

Cited by 162 publications

References 24 publications

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Atopic Dermatitis

Increased Expression of RANTES, CCR3 and CCR5 in the Lesional Skin of Patients with Atopic Eczema

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