ATP autocrine/paracrine signaling induces calcium oscillations and NFAT activation in human mesenchymal stem cells

Kawano, Seiko; Otsu, Keishi; Kuruma, Akinori; Shoji, Shuichi; Yanagida, Eri; Muto, Yuko; Yoshikawa, Fumio; Hirayama, Yoshiyuki; Mikoshiba, Katsuhiko; Furuichi, Teiichi

doi:10.1016/j.ceca.2005.11.008

Cited by 141 publications

(166 citation statements)

References 49 publications

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“…oscillations Ca 2+ oscillations in human MSCs have been reported to be driven by an autocrine/paracrine purinergic loop, whereby ATP released through hemi-gap junctions triggered P2Y1 metabotropic purinergic receptors and associated PLC-β and IP3R signalling [22]. More recently, we have implicated a similar autoregulatory pathway in differentiating cells of chicken chondrogenic cell cultures, with P2X4 ionotropic purinergic receptor being the most likely candidate [10,11].…”

Section: Purinergic Signalling In Cpcs and Autocrine/paracrine Regulamentioning

confidence: 85%

“…Ca 2+ oscillations have been reported in a great variety of non-excitable cells including pancreatic β-cells [49], embryonic stem cells (ESCs) [47], chondroblasts [11,35], chondrocytes [40] and human MSCs [22,24]. This is the first study to report that human chondroprogenitor cells derived from regions with minor lesions of osteoarthritic articular cartilage (CPCs) are also characterised by a similarly dynamic Ca 2+ homeostasis.…”

Section: Dynamics Of Cytosolic [Ca 2+ ]Imentioning

confidence: 95%

“…Since periodic Ca 2+ transients in human mesenchymal stem cells (MSCs) could be attributed to an autocrine/paracrine purinergic signalling loop [22], and as the ionotropic purinoreceptor P2X4 was proved to play a role in differentiation of chicken chondroprogenitor cells [10], we hypothesised the presence of a similar purinergic signal transduction mechanism also in migratory chondroprogenitor cells. To investigate the possibility of such regulatory systems, we first carried out a comprehensive mRNA transcript screening for all purinergic receptor mRNAs involved in Ca 2+ homeostasis and found that of the A1 adenosine receptor subtypes A1, A2A and A2B, but not A3 adenosine receptor transcripts; of the P2X ionotropic purinergic receptor subtypes P2X1, P2X4, P2X5, P2X6 and P2X7, but not P2X2 and P2X3; and mRNAs for all Gq-coupled P2Y metabotropic purinergic receptor subtypes were detectable using RT-PCR (see Table 1; mRNA expression of other P2Y receptor subtypes are shown in the Online Resource in Table S4 and Fig.…”

Section: Extracellular Administration Of Nucleotides Evoke Ca 2+ Tranmentioning

confidence: 99%

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Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Matta

Fodor

Miosge

et al. 2014

Pflugers Arch - Eur J Physiol

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Osteoarthritis (OA) is the most common form of chronic musculoskeletal disorders. A migratory stem cell population termed chondrogenic progenitor cells (CPC) with in vitro chondrogenic potential was previously isolated from OA cartilage. Since intracellular Ca 2+ signalling is an important regulator of chondrogenesis, we aimed to provide a detailed understanding of the Ca 2+ homeostasis of CPCs. In this work, CPCs immortalised by lentiviral administration of the human telomerase reverse transcriptase (hTERT) and grown in monolayer cultures were studied. Expressions of all three IP3Rs were confirmed, but no RyR subtypes were detected. Ca 2+ oscillations observed in CPCs were predominantly dependent on Ca 2+ release and store replenishment via store-operated Ca 2+ entry; CPCs express both STIM1 and Orai1 proteins. Expressions of adenosine receptor mRNAs were verified, and adenosine elicited Ca 2+ transients. Various P2 receptor subtypes were identified; P2Y1 can bind ADP; P2Y4 is targeted by UTP; and ATP may evoke Ca 2+ transients via detected P2X subtypes, as well as P2Y1 and P2Y2. Enzymatic breakdown of extracellular nucleotides by apyrase completely abrogated Ca 2+ oscillations, suggesting that an autocrine/paracrine purinergic mechanism may drive Ca 2+ oscillations in these cells.As CPCs possess a broad spectrum of functional molecular elements of Ca 2+ signalling, Ca 2+ -dependent regulatory mechanisms can be supposed to influence their differentiation potential.

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Section: Purinergic Signalling In Cpcs and Autocrine/paracrine Regulamentioning

confidence: 85%

Section: Dynamics Of Cytosolic [Ca 2+ ]Imentioning

confidence: 95%

Section: Extracellular Administration Of Nucleotides Evoke Ca 2+ Tranmentioning

confidence: 99%

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Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Matta

Fodor

Miosge

et al. 2014

Pflugers Arch - Eur J Physiol

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“…Non-stimulated cells in culture and intact tissues often display spontaneous [Ca 2+ ] i oscillations [88,[116][117][118][119] [109,117,118]. Taken together, these findings suggest that spontaneous [Ca 2+ ] i oscillations in different cellular systems may reflect the basal property of cells to release and sense ATP.…”

Section: Intracellular Ca 2+ Oscillationsmentioning

confidence: 99%

ATP release from non-excitable cells

Praetorius

Leipziger

2009

Purinergic Signalling

207

229

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All cells release nucleotides and are in one way or another involved in local autocrine and paracrine regulation of organ function via stimulation of purinergic receptors. Significant technical advances have been made in recent years to quantify more precisely resting and stimulated adenosine triphosphate (ATP) concentrations in close proximity to the plasma membrane. These technical advances are reviewed here. However, the mechanisms by which cells release ATP continue to be enigmatic. The current state of knowledge on different suggested mechanisms is also reviewed. Current evidence suggests that two separate regulated modes of ATP release co-exist in nonexcitable cells: (1) a conductive pore which in several systems has been found to be the channel pannexin 1 and (2) vesicular release. Modes of stimulation of ATP release are reviewed and indicate that both subtle mechanical stimulation and agonist-triggered release play pivotal roles. The mechano-sensor for ATP release is not yet defined.

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“…The Ca 2+ homeostasis of MSCs has been mapped earlier and Ca 2+ oscillations have been reported to be driven by an autocrine/paracrine purinergic loop, whereby ATP released through hemi-gap junctions triggered P2Y 1 metabotropic purinergic receptors and Ca 2+ release via PLC-β and IP 3 R signalling [94]. In migratory CPCs, we have recently confirmed the mRNA and protein level expression of certain P2X (P2X 1,4,5,6,7 , but not P2X 2 and P2X 3 ) and all P2Y receptors [86].…”

Section: P2 Purinergic Receptor Expression and Function During Chondrmentioning

confidence: 99%

Purinergic signalling-evoked intracellular Ca2+ concentration changes in the regulation of chondrogenesis and skeletal muscle formation

et al. 2016

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ATP autocrine/paracrine signaling induces calcium oscillations and NFAT activation in human mesenchymal stem cells

Cited by 141 publications

References 49 publications

Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

Purinergic signalling is required for calcium oscillations in migratory chondrogenic progenitor cells

ATP release from non-excitable cells

Purinergic signalling-evoked intracellular Ca2+ concentration changes in the regulation of chondrogenesis and skeletal muscle formation

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