ATP citrate lyase is required for normal sexual and asexual development in Gibberella zeae

FgVelB globally regulates sexual reproduction, mycotoxin production and pathogenicity in the cereal pathogen Fusarium graminearum

“…A member (FGSG_08081) of the gene cluster involved in butenolide biosynthesis in F. graminearum (Harris et al, 2007) was downregulated only in the DFgVelB strain. In addition, two ATP citrate lyase genes [ACL1 (FGSG_12857) and ACL2 (FGSG_06039)], required for production of acetylCoA (Son et al, 2011a), a building block for polyketide biosynthesis, were downregulated in the DFgVelB strain (Fig. 9).…”

“…The virulence of fungal strains was determined on wheat and barley, as described previously (Son et al, 2011a). Briefly, conidia of each strain were harvested from carrot agar and suspended in sterile water at a concentration of 1610 5 conidia ml 21 .…”

Estrogenic Compounds Compatible with a Conditional Gene Expression System for the Phytopathogenic Fungus Fusarium graminearum

“…The replacement of the ACS2 promoter with Pzear in the ACL2 deletion mutant resulted in a severe reduction in mycelia growth but its defect was partially recovered with the addition of ZEA to the growth medium (Lee et al, 2011a). In the present study, the same strategy was used to replace the ACS2 promoter with Pzear in the ACL2 deletion mutant (Δacl2ACS2p) (Son et al, 2011), yet β-estradiol was used instead of ZEA. In brief, the hyg-pzear fragment that carries a hygromycin-resistant gene fused with Pzear was amplified from the Pzear-GzmetE strain (Lee et al, 2010b) using HYG-F1 and zear-r2 primers.…”

“…Since the whole genome sequences of F. graminearum were publicly released by the Broad Institute in 2003 (http:/ /www.broadinstitute.org/), many research groups have performed functional studies to identify genes related to pathogenicity (Ding et al, 2009;Seong et al, 2005), sexual and asexual development (Harris, 2005;Min et al, 2010;Lee et al, , 2010aSon et al, 2011), and secondary metabolite biosynthesis pathways (Gaffoor and Trail, 2006;Kim et al, 2005Kim et al, , 2006Lysøe et al, 2006). While molecular manipulation tools such as targeted gene deletion and gene over-expression have been well developed, conditional gene expression systems that allow the expression of certain genes under desired conditions have been limited in F. graminearum.…”

A novel gene, GEA1, is required for ascus cell-wall development in the ascomycete fungus Fusarium graminearum

“…To date, several genes and pathways have been reported to play critical roles in the sexual development of F. graminearum, including mating-type genes (Desjardins et al, 2004;Lee et al, 2003), signal transduction pathways (Hou et al, 2002;Jenczmionka et al, 2003;Nguyen et al, 2012;Park et al, 2012;Yu et al, 2008), calcium metabolism (Cavinder & Trail, 2012;Cavinder et al, 2011;Hallen & Trail, 2008) and acetyl CoA metabolism Son et al, 2011aSon et al, , 2012b. Recently, large-scale functional analyses of transcription factors and kinase genes identified hundreds of genes that are involved in specific steps of perithecium development in F. graminearum (Son et al, 2011c;Wang et al, 2011).…”