ATP leakage from yeast cells treated by extracellular glycolipids of

Kulakovskaya, T. V.; Kulakovskaya, Ekaterina; Golubev, W. I.

doi:10.1016/s1567-1356(02)00202-7

Cited by 42 publications

(32 citation statements)

References 12 publications

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“…Intracellular substances leakages can indicate membrane damage (Yeaman et al, 1998;Johnston et al, 2003;Kulakovskaya et al, 2003;Higgins et al, 2005). As the central energy carrier of all organisms, ATP loss also can be the indicator of membrane damage (Paul et al, 2010;Zhang et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Alteration in Escherichia coli and Streptococcus faecalis cells induced by ozone

2017

SDRP-JFST

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ABSTRACT:To elucidate ozone action mechanism, cell suspensions of E. coli 8099 and S. faecalis ATCC29212 were initially exposed to ozone for 5 min, and then treated with sodium dodecyl sulfate, sodium hydroxide, and proteinase K. those of S. faecalis were 7.95±2.81%-55.85±1.43%. After ozone pre-treatment, OD600 value of E. coli cells, sequently treated with SDS, NaOH, and PK, decreased by 8.83±1.86%, 34.72±0.42%, and 10.42±2.08%, respectively. And that of S. feacalis decreased by 2.85±3.63%, 71.30±1.08%, and 7.00±2.47%, respectively. Therefore, ozone pre-treatment made E. coli cells be sensitive to SDS and NaOH, not susceptible to PK. It was confirmed by transmission electron microscopy of E. coli cells. To S. faecalis, ozone pre-treatment activated the effects of SDS, NaOH, and PK on cells. ATP loss from S. faecalis cells indicated that ozone damaged cell membrane and increased membrane permeability. Because SDS-, NaOH-, and PK-induced cell lyses were respectively due to protein denaturation, cell membrane damage, and the reactions between PK and proteins or conjugated proteins, it was concluded that protein changes and cell membrane damage generated by ozone are responsible for E. coli and S. faecalis inactivation. Results showed that, after ozone treatment, OD600 reduction rates of E. coli cells were 6.11±1.29%-7.06±1.23%.

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Section: Discussionmentioning

confidence: 99%

Alteration in Escherichia coli and Streptococcus faecalis cells induced by ozone

2017

SDRP-JFST

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“…Then cell precipitates were incubated with 0.05 mL of dimethylsulfoxide for 30 min at room temperature. The extracts were used for the luciferin-luciferase assay of ATP as described 18 .…”

Section: Mic Assaymentioning

confidence: 99%

“…The convenient method for assessing membrane-damaging activity is ATP assay 18 . ATP content in the cells of F. neoformans was measured before and after the treatment with glycolipids Fig.…”

Section: Membrane-damaging Activities Of Glycolipidsmentioning

confidence: 99%

The Antibiotic and Membrane-damaging Activities of Cellobiose Lipids and Sophorose Lipids

2014

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“…In addition, the cellobiose is esterified at several positions with either acetyl or medium-chain hydroxy fatty acids (30). Cellobiose-derived glycolipids have since been detected in other fungal species (26,34). The fungal biocontrol agent Pseudozyma flocculosa produces flocculosin, a rare cellobiose lipid with antifungal activity (9).…”

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confidence: 99%

Genetic Analysis of Biosurfactant Production inUstilago maydis

Hewald

Josephs

Bölker

2005

Appl Environ Microbiol

140

124

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The dimorphic basidiomycete Ustilago maydis produces large amounts of surface-active compounds under conditions of nitrogen starvation. These biosurfactants consist of derivatives of two classes of amphipathic glycolipids. Ustilagic acids are cellobiose lipids in which the disaccharide is O-glycosidically linked to 15,16-dihydroxyhexadecanoic acid. Ustilipids are mannosylerythritol lipids derived from acylated ␤-D-mannopyranosyl-D-erythritol. Whereas the chemical structure of these biosurfactants has been determined, the genetic basis for their biosynthesis and regulation is largely unknown. Here we report the first identification of two genes, emt1 and cyp1, that are essential for the production of fungal extracellular glycolipids. emt1 is required for mannosylerythritol lipid production and codes for a protein with similarity to prokaryotic glycosyltransferases involved in the biosynthesis of macrolide antibiotics. We suggest that Emt1 catalyzes the synthesis of mannosyl-D-erythritol by transfer of GDP-mannose. Deletion of the gene cyp1 resulted in complete loss of ustilagic acid production. Cyp1 encodes a cytochrome P450 monooxygenase which is highly related to a family of plant fatty acid hydroxylases. Therefore we assume that Cyp1 is directly involved in the biosynthesis of the unusual 15,16-dihydroxyhexadecanoic acid. We could show that mannosylerythritol lipid production is responsible for hemolytic activity on blood agar, whereas ustilagic acid secretion is required for long-range pheromone recognition. The mutants described here allow for the first time a genetic analysis of glycolipid production in fungi.

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ATP leakage from yeast cells treated by extracellular glycolipids of

Cited by 42 publications

References 12 publications

Alteration in Escherichia coli and Streptococcus faecalis cells induced by ozone

Alteration in Escherichia coli and Streptococcus faecalis cells induced by ozone

The Antibiotic and Membrane-damaging Activities of Cellobiose Lipids and Sophorose Lipids

Genetic Analysis of Biosurfactant Production inUstilago maydis

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