ATR-FTIR Spectroscopy Reveals Bond Formation During Bacterial Adhesion to Iron Oxide

Abstract

The contribution of various bacterial surface functional groups to adhesion at hematite and ZnSe surfaces was examined using attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy. When live Shewanella oneidensis, Pseudomonas aeruginosa, and Bacillus subtilis cells were introduced to a horizontal hematite (alpha-Fe(2)O(3))-coated internal reflection element (IRE), FTIR peaks emerged corresponding to bacterial phosphate group binding. These IR peaks were not observed when bacteria were introduced to the uncoated ZnSe IRE. When cells were added to colloidal suspensions of alpha-Fe(2)O(3) at pH 7, spectra included peaks corresponding to P-OFe and nu(COOH), the latter being attributed to bridging of carboxylate at mineral surface OH groups. Selected model organic compounds with P-containing functionalities (phenylphosphonic acid [PPA], adenosine 5'-monophosphate [AMP], 2'-deoxyadenyl(3'-->5')-2'-deoxyadenosine [DADA], and deoxyribonucleic acid [DNA]) produce spectra with similar peaks corresponding to P-OFe when adsorbed to alpha-Fe(2)O(3). The data indicate that both terminal phosphate/phosphonate and phosphodiester groups, either exuded from the cell or present as surface biomolecules, are involved in bacterial adhesion to Fe-oxides through formation of innersphere Fe-phosphate/phosphonate complexes.

Microscale scavenging of pentachlorophenol in water using amine and tripolyphosphate-grafted SBA-15 silica: Batch and modeling studies

“…These peaks are associated with phosphate and phosphoramide groups, and it suggests reaction between eP 3 O 5À 10 and eNH þ 3 of the APS group. Similar observations have been reported by Panda et al (2003), Parikh and Chorover (2006), and Lee et al (2001).…”

Arsenic interception by cell wall of bacteria observed with surface-enhanced Raman scattering

“…2 shows the FTIR spectra of whole cell samples of eight bacteria in the wavenumber range of 900-1800 cm − 1 . These spectra exhibited characteristic peaks similar to reported data for different microorganisms (Gao and Chorover, 2009;Parikh and Chorover, 2006;Shephard et al, 2008). The assignment of FTIR bands is shown in Table S1.…”

Competition between sodium oleate and sodium silicate for a silicate/oleate modified magnetite surface studied by in situ ATR-FTIR spectroscopy

“…Attenuated Total Reflection (ATR) infrared spectroscopy was utilized, a technique that has been widely applied to study surface adsorption from aqueous solution onto finely dispersed solids including metal oxides e.g. [2][3][4][5][6][7][8]. ATR spectroscopy has also been used to study surface reactions related to flotation [9][10][11][12][13][14][15].…”