Atrazine triggers hepatic oxidative stress and apoptosis in quails (Coturnix C. coturnix) via blocking Nrf2-mediated defense response

Cong, Zhen; Li, Xue-Nan; Xiang, Li-Run; Qin, Lei; Lin, Jianhua; Li, Jinlong

doi:10.1016/j.ecoenv.2016.11.016

Cited by 57 publications

(27 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two-week’s continuous stimulation was given in our study, which opposite to short-term stress was given in several Nrf2-related studies [ 41 – 43 ]. Moreover, it was suggested that persistent accumulation of Nrf2 in the nucleus has toxic effects, such as inducing apoptosis and aggravating oxidative stress [ 44 , 45 ]. So such effects might have caused negative feedback, leading to decreased Nrf2 in the 2-week cold exposed group.…”

Section: Discussionmentioning

confidence: 99%

Cold exposure induced oxidative stress and apoptosis in the myocardium by inhibiting the Nrf2-Keap1 signaling pathway

Cong

Liu

et al. 2018

BMC Cardiovasc Disord

View full text Add to dashboard Cite

BackgroundExposure to cold weather is associated with infaust cardiovascular responses, including myocardial infarction and arrhythmias. However, the exact mechanisms of these adverse changes in the myocardium under cold stress are unknown. This study was designed to investigate the mechanisms of cardiac injury induced by cold stress in mice.MethodsThe mice were randomly divided into three groups, normal control (no handling), 1-week cold stress and 2-week cold stress. We observed physiological changes of the mice and morphological changes of myocardium tissues, and we measured the changes of 3′-nitrotyrosine and 4-hydroxynonenal, the expression levels of superoxide dismutase-1, superoxide dismutase-2, Bax, Bad, Bcl-2, Nuclear factor erythroid-derived 2-like 2 (Nrf2) and Kelch like-ECH-associated protein 1 (Keap1) in myocardium by western blot. Besides, we detected mRNA of superoxide dismutase-1, superoxide dismutase-2, Bax, Bad, Bcl-2, Nrf2 and Keap1 by real-time PCR. One-way analysis of variance, followed by LSD-t test, was used to compare each variable for differences among the groups.ResultsEchocardiography analyses demonstrated left ventricle dysfunction in the groups receiving cold stress. Histological analyses witnessed inflammation, vacuolar and eosinophilic degeneration occurred in left ventricle tissues. Western blotting results showed increased 3′-nitrotyrosine and 4-hydroxynonenal and decreased antioxidant enzymes (superoxide dismutase-1 and superoxide dismutase-2) in the myocardium. Expression of Nrf2 and Keap1 followed a downward trend under cold exposure, as indicated by western blotting and real-time PCR. Expression of anti-apoptotic protein Bcl-2 also showed the same trend. In contrast, expression of pro-apoptotic proteins Bax and Bad followed an upward trend under cold exposure. The results of real-time PCR were consistent with those of western blotting.ConclusionsThese findings were very significant, showing that cold exposure induced cardiac injury by inhibiting the Nrf2-Keap1 signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Cold exposure induced oxidative stress and apoptosis in the myocardium by inhibiting the Nrf2-Keap1 signaling pathway

Cong

Liu

et al. 2018

BMC Cardiovasc Disord

View full text Add to dashboard Cite

show abstract

“…ATR in water has been identified as a threat to the animal ecosystem through the food chain . So the global researchers are mainly concerned and interested in studying the adverse effects of ATR in the wildlife and experimental animals . Furthermore, most of these studies evaluated the effects of ATR exposure in cell, tissue and organs .…”

Section: Discussionmentioning

confidence: 99%

“…34 So the global researchers are mainly concerned and interested in studying the adverse effects of ATR in the wildlife and experimental animals. 35 Furthermore, most of these studies evaluated the effects of ATR exposure in cell, tissue and organs. 36,37 However, only a few studies investigated the mechanisms of the hepatotoxicity of ATR exposure in quails.…”

Section: Discussionmentioning

confidence: 99%

Activating nuclear xenobiotic receptors and triggering ER stress and hepatic cytochromes P450 systems in quails (Coturnix C. coturnix) during atrazine exposure

Qin

Lin

et al. 2017

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Atrazine (ATR) is one of the most widely detected contaminant in the ecosystem. Nuclear xenobiotic receptors are activated by herbicides and induce the transcription of CYP450 isoforms involved in xenobiotic metabolism and transport. However, little is known about hepatic nuclear xenobiotic receptors in birds are responsible for ATR-induced hepatotoxicity via regulating the cytochrome P450 enzyme systems (CYP450s). The objective of this study was to investigate the mechanism of ATR hepatotoxicity in quails. For this purpose, male quails were dosed by oral gavage from sexual immaturity to maturity with 0, 50, 250, and 500 mg/kg/day ATR for 45 days. The results showed that ATR exposure caused the hepatotoxicity damage and endoplasmic reticulum (ER) degeneration. It suggested that ER is a target organelle of ATR toxicity in hepatocytes. ATR exposure disrupted the hepatic CYP450s homeostasis. This study also demonstrated that ATR triggered the CYP450 isoforms transcription via activating the hepatic CAR/PXR pathway. The present study provides new insights regarding the mechanism of the ATR-induced hepatotoxicity through activating nuclear xenobiotic receptors and triggering ER stress and hepatic CYP450s in quails.

show abstract

“…The mean body and liver weight in ATZ treated mice showed a highly significant reduction (p<0.001) compared to the Cnt group. Decreased body and liver weight in mice might be attributed to the atrazine-generated oxidative stress that affected animals' general body health (Zhang et al, 2017).…”

Section: Nuclear Diameter Of Hepatocytes (µM)mentioning

confidence: 99%

Atrazine Induced Histopathological Alterations in the Liver of Adult Male Mice

Batool¹,

Batool²,

Shameem³

et al. 2021

PUJZ

View full text Add to dashboard Cite

Michael et al., 2018). Atrazine is very environmentally stable due to low loss by volatilization and degradation. Furthermore, because of its accumulation effect, atrazine is significantly affecting the health of many organisms, including humans (Nodler et al., 2013;Juhel et al., 2017). European Union banned Atrazine in 2003 due to its severe toxicity to cells and tissues. However, the United States Environmental Protection Agency (USEPA) still permits the use of atrazine worldwide (Almberg et al., 2018). Various studies have shown that Atrazine interferes with the normal functioning of body organs, including the

show abstract

Atrazine triggers hepatic oxidative stress and apoptosis in quails (Coturnix C. coturnix) via blocking Nrf2-mediated defense response

Cited by 57 publications

References 52 publications

Cold exposure induced oxidative stress and apoptosis in the myocardium by inhibiting the Nrf2-Keap1 signaling pathway

Cold exposure induced oxidative stress and apoptosis in the myocardium by inhibiting the Nrf2-Keap1 signaling pathway

Activating nuclear xenobiotic receptors and triggering ER stress and hepatic cytochromes P450 systems in quails (Coturnix C. coturnix) during atrazine exposure

Atrazine Induced Histopathological Alterations in the Liver of Adult Male Mice

Contact Info

Product

Resources

About