Atribuição inequívoca de deslocamentos químicos de RMN de ¹H e 13C de plumierídeo isolado da Allamanda cathartica

Malheiros, Ângela; Schuquel, Ivânia T.A.; Vidotti, Gentil J.

doi:10.1590/s0100-40421997000500002

Cited by 7 publications

(7 citation statements)

References 3 publications

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“…The purity of the compound was assessed by high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) analysis, through comparison with the literature and direct comparison with original samples. 13,21,35 Plumieride presented a purity > 98%. Methanol and acetonitrile were purchased from Tedia® (HPLC grade).…”

Section: Chemicalsmentioning

confidence: 93%

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DEVELOPMENT AND OPTIMIZATION OF A MICROWAVE-ASSISTED EXTRACTION OF PLUMIERIDE FROM Allamanda cathartica L. FLOWERS

Bonomini¹,

Góes²,

Machado³

et al. 2017

Quim. Nova

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A simple and efficient microwave-assisted extraction process has been developed in order to extract plumieride from Allamanda cathartica flowers. The extracts have been obtained through microwave-assisted extraction (MAE) process with ethanol and ethyl acetate at different time, power and temperatures. Static maceration at room temperature with ethanol and ethyl acetate was obtained in order to compare the efficiency of extraction. HPLC method was validated to quantify plumieride in the extracts. The analyzed parameters: linearity (R 2 = 0.999), precision (CV 3.3%), recovery (98%), specificity (97%), limit of detection (1 µg mL -1), and limit of quantification (2 µg mL -1 ) were satisfactory. The yield of plumieride reached 43% in the extracts under the optimal MAE conditions (10 min, 300W). Plumieride recoveries by conventional extraction method were 12% with ethanol and 22% with ethyl acetate. Time and power parameters on plumieride extraction yield was determined by applying a factorial design and surface plots. Ethanol was the best extractor solvent and it was possible to obtain high contents (52 mg g -1 of dry flowers) at optimized conditions. It can be concluded that MAE process has actual advantages over the conventional extraction method in terms of shorter time and higher efficiency to recover plumieride from A. cathartica flowers.

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Section: Chemicalsmentioning

confidence: 93%

“…The compound was analyzed by HPLC and NMR, then the data were compared with those found in the literature or with data of the standard previously isolated. 13,21,35 …”

Section: Extraction and Isolation Of Plumieridementioning

confidence: 99%

DEVELOPMENT AND OPTIMIZATION OF A MICROWAVE-ASSISTED EXTRACTION OF PLUMIERIDE FROM Allamanda cathartica L. FLOWERS

Bonomini¹,

Góes²,

Machado³

et al. 2017

Quim. Nova

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“…All the compounds were identified based on their spectral data, by comparison with the literature (Malheiros et al 1997, Schmidt et al, 2006 and direct comparison with original samples.…”

Section: Isolation and Identification Of Componentsmentioning

confidence: 99%

“…From this procedure a mixture of -sitosterol and stigmasterol, plumericin, plumieride, ursolic acid, 3-O--glucopyranosylsitosterol and a mixture of sugars were obtained. The compounds were analyzed by nuclear magnetic resonance (NMR) and their chemical shifts were compared with those found in the literature or with data of the standards previously isolated (Ganapaty et al, 1988, Abdel-Kader et al, 1997, Malheiros et al, 1997, Schmidt et al, 2006.…”

Section: Identification Of Active Compounds In Dcm Fractionmentioning

confidence: 99%

Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii

Nascimento

Faqueti

Wilhelm

et al. 2014

Revista Brasileira de Farmacognosia

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Chromatography Iridoids K562 and Nalm6 cells Plumericin A B S T R A C T The aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii, Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The resultsclearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor com-pounds not yet identified. The seasonal influence on the production of secondary metabolites was verified.

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“…The product HA1 was visualized under visible and UV light (254 nm) and submitted to nuclear magnetic resonance ( 1 H NMR and 13 C NMR) analyses. The chemical structure of isolated compound was identified by nuclear magnetic resonance (Bruker 400) using 1 H NMR (400 MHz), 13 C NMR (100 MHz), 2D NMR analysis, and by comparison of spectral data with those available in the literature (Malheiros et al, 1997). MeOD was used as solvent for HA1.…”

Section: Isolation and Structure Elucidation Of Ha1mentioning

confidence: 99%

Antiproliferative effect of a traditional remedy, Himatanthus articulatus bark, on human cancer cell lines

Rebouças

Grivicich

Santos

et al. 2011

Journal of Ethnopharmacology

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Atribuição inequívoca de deslocamentos químicos de RMN de ¹H e 13C de plumierídeo isolado da Allamanda cathartica

Cited by 7 publications

References 3 publications

DEVELOPMENT AND OPTIMIZATION OF A MICROWAVE-ASSISTED EXTRACTION OF PLUMIERIDE FROM Allamanda cathartica L. FLOWERS

DEVELOPMENT AND OPTIMIZATION OF A MICROWAVE-ASSISTED EXTRACTION OF PLUMIERIDE FROM Allamanda cathartica L. FLOWERS

Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii

Antiproliferative effect of a traditional remedy, Himatanthus articulatus bark, on human cancer cell lines

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