Attachment and biofilm formation by foodborne bacteria in meat processing environments: Causes, implications, role of bacterial interactions and control by alternative novel methods

Giaouris, Efstathios; Heir, Even; Hébraud, Michel; Chorianopoulos, Nikos; Langsrud, Solveig; Møretrø, Trond; Habimana, Olivier; Desvaux, Mickaël; Rénier, Sandra; Nychas, George‐John E.

doi:10.1016/j.meatsci.2013.05.023

Cited by 303 publications

(218 citation statements)

References 263 publications

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“…Recently, prospective treatment alternatives have been tested for biofilm-related infections, particularly in the food sector. Bacteriophages, bacteriocin, titanium dioxide photocatalysts, ionization or ultraviolet radiation, surfactant treatment, ultrasonic treatment, ozone, microemulsion, and nanoemulsion can be given as examples of such new methods of treatment (29).…”

Section: Discussionmentioning

confidence: 99%

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Köse¹,

Yapar²

2017

Turk J Med Sci

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IntroductionA biofilm can be defined as a well-organized microbial community in the extracellular polymeric substance (EPS) that adheres to living or inanimate surfaces (1). Owing to their resistance to antibiotics and antiphagocytic effects, biofilms can frequently cause persistent chronic infections that are difficult to treat. Biofilms are significant causes of morbidity and mortality (2,3). At least 65% of all bacterial infections are associated with biofilm (2,3). Infections such as natural valve endocarditis, otitis media, chronic bacterial prostatitis, cystic fibrosis, and periodontitis develop as a result of biofilms that form on living surfaces. The medical devices on which biofilms can develop include prosthetic heart valves, central venous catheters, urinary catheters, contact lenses, and intrauterine devices (4). Due to the high rates of mortality and morbidity associated with biofilms, several studies have been conducted on antimicrobials and particularly on the effectiveness of antibiotics against biofilms. It has been demonstrated that antibiotics show limited efficacy against biofilms as the biofilm layer persists. For this reason, it should be borne in mind that disinfectants serve as a significant alternative against human mucosa and biofilms on the surfaces of medical device (5). This study aims at investigating the effectiveness of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite against Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and the live microbial cells on the biofilm layers. Material and methods Bacterial strainsS. aureus (ATCC 6538) and P. aeruginosa (PA01) bacterial strains that are known to produce biofilms were selected (5,6). Bacterial cells stocked at -80 °C were passaged to a tryptic soy agar (TSA) plate (Merck). After being incubated for 24 h at 35-37 °C, a bacterial solution was Background/aim: Because biofilms are resistant to antibiotics and biocides, they usually cause chronic persistent infections, which are arduous to cure and have high mortality and morbidity. Our study aimed to investigate the efficiency of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite on Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and live microbial cells.Materials and methods: Biofilm layers were determined by crystal violet assay and live microbial cells were determined using a resazurin assay.Results: For Pseudomonas aeruginosa, sodium hypochlorite showed the most influential disinfection because it diminished 83.6% of the biofilm layer and decreased 99.7% of live microbial cells. For Staphylococcus aureus, hydrogen peroxide was determined the most active disinfectant with 80.3% reduction of the biofilm layer. Sodium hypochlorite was also determined to be the most efficient disinfectant with 99.8% reduction of live microbial cells. Sodium hypochlorite was the most influential disinfectant on biofilm layers and live microbial cells of both microorganisms. Conclusion:We concluded that if we use sodi...

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Section: Discussionmentioning

confidence: 99%

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Köse¹,

Yapar²

2017

Turk J Med Sci

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“…Salmonella Typhi bacteria cause human deaths all over the world, resulting in a notification rate of 20.4 cases per population of 100,000 [1]. Salmonella Typhi is commonly detected in animal-food products such as eggs, poultry and meat [2], fresh vegetables and processed fruit [3]. Salmonella is also detected in surface water and drinking water [1].…”

Section: Introductionmentioning

confidence: 99%

Disinfection Performance against Salmonella Typhi in Water by Radio Frequency Inductive Couple Plasma System

Desmiarti

Hazmi

2017

J. Eng. Technol. Sci.

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Abstract. The disinfection performance of the radio frequency inductively coupled plasma (RFICP) system against Salmonella Typhi in water was examined using continuous flow experiments. The evaluation was based on disinfection efficiency, death rate constant, disinfection yield, and energy consumption. For all experiments the electromagnetic flux generated in the plasma reactor varied from 4 to 6 W/cm 2 . The disinfection efficiency and death rate constant of Salmonella Typhi decreased with the increase of the initial number of Salmonella Typhi bacteria. The disinfection yield increased from 784 to 1889 CFU/KWh and the energy consumption decreased from 0.28 to 0.07 KWh/L with the flowrate increasing from 5 to 20 mL/minute. The flowrate is an important parameter in predicting disinfection performance against pathogenic bacteria in water to design drinking water treatment plants.

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“…However, the efficient cleaning and disinfecting procedures are the main requirements because many food pathogenic and spoilage bacteria are able to attach to food contacting surfaces [3,4,5]. Moreover, they might still remain viable even after cleaning and disinfection which might lead to food contamination [2,4,[6][7][8]. Among many reasons causing food contamination, microbial biofilm is important.…”

Section: Introductionmentioning

confidence: 99%

“…Mixed-species biofilms are usually more stable than mono-species biofilms. Cell-to-cell interactions have been demonstrated to play a key role in biofilm formation, biofilm structure, as well as in the resistance of biofilm community members against antimicrobial treatments [4,[11][12][13]. That is the reason why cells in biofilm are significantly resistant to sanitizers compared with the same cells being planktonic [4,8,14].…”

Section: Introductionmentioning

confidence: 99%

“…However, limited research has been published related to the microbiology of food processing surfaces even though microbial contamination in the processing environment is an important parameter. The microorganisms present are likely a mixture of many species [4,22] and some of these microorganisms produce exopolymers that can facilitate the retention of pathogenic microorganism [5]. Understanding the characteristics of adhered microflora on food processing equipment would provide valuable information in designing the cleaning and disinfecting procedures.…”

Section: Introductionmentioning

confidence: 99%

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Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant

Hoa

Mahakarnchanakul²,

Sajjaanantakul³

et al. 2015

JFNS

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Abstract:Adhering microflora profile is essential for the development and design of cleaning and sanitizing procedures, which is a part of the HACCP system. The profile provides a complete view of the target microorganisms that can potentially contaminate food products. The aim of this study is, therefore, focused on examination of microbial population adhered to the equipment surfaces at seafood processing plant. Adhering microflora was evaluated by installing the stainless steel coupons on the surface of equipment. Seventy stainless steel coupons were attached on 7 different locations at seafood processing plant and then fourteen coupons were collected at 1, 2, 3, 5 and 9 weeks interval. The population of bacteria adhered on samples were enumerated using swab test and spread plate method on different selective microbiological media. The total viable count and Pseudomonas spp. population found on the stainless steel coupons were ranged from 10 1 to 10 6 cfu/cm 2 . The results also indicated that mature biofilm might form at some locations after three weeks. After 5 weeks, the highest viable bacterial population (10 6 cfu/cm 2 ) was observed on the stainless steel coupons attached in the trolley used for carrying the soup. These finding supported that the appropriate cleaning and sanitizing procedure should be strictly applied to assure safety in food processing plant.

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Attachment and biofilm formation by foodborne bacteria in meat processing environments: Causes, implications, role of bacterial interactions and control by alternative novel methods

Cited by 303 publications

References 263 publications

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Disinfection Performance against Salmonella Typhi in Water by Radio Frequency Inductive Couple Plasma System

Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant

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