Attachment and release of spermatozoa from the caudal isthmus of the hamster oviduct

Smith, T. Timothy; Yanagimachi, Ryuzo

doi:10.1530/jrf.0.0910567

Cited by 185 publications

(115 citation statements)

References 13 publications

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“…In the bull [7,30], horse [37], pig [16], rabbit [27], hamster [32] and mouse [34], it has been observed that sperm transported to the oviduct through the uterine horn attach to the OEC to maintain motility and viability. The OEC is known to secrete substances that support and maintain sperm motility and viability [1,17,21].…”

Section: Discussionmentioning

confidence: 99%

Induction of Dog Sperm Capacitation by Oviductal Fluid.

Kawakami

Hori

Tsutsui

1998

The Journal of Veterinary Medical Science

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ABSTRACT. Four estrous beagles were inseminated with 1 × 10 8 sperm into both the right and left uterine horns, and the uterine horn and oviduct on one side were removed under anesthesia after 7 hr and 24 hr, respectively. The lumen of the uterine horns and oviducts was flushed with canine capacitation medium (CCM), and movement of the sperm in CCM was assessed by phase-contrast microscopy. In a second experiment, ejaculated sperm obtained from 5 normal beagles was incubated in CCM supplemented with oviductal flush fluid (OF-CCM) at 38°C with 5% CO 2 in air. Motility of sperm, and percentages of hyperactivated sperm (%HA) and acrosome-reacted sperm (%AR) among freely swimming (FS) sperm were investigated until 24 hr after the start of incubation. After 7 hr of incubation the sperm was coincubated with canine oocytes in OF-CCM for 2 hr, and the number of zona pellucida-binding (zona-binding) sperm was then counted. The %HA among the sperm in the oviductal flush fluid both 7 hr (mean ± S.E.; 15.0 ± 2.4%) and 24 hr (77.5 ± 5.2%) after intrauterine insemination were significantly higher than in the uterine flush fluid (P<0.05, 0.01, respectively). The motility and %HA among FS-sperm in OF-CCM were higher than in the control medium without oviductal fluid. However, there was no difference in the %AR between OF-CCM and control medium. The number of zona-binding sperm in OF-CCM (8 ± 1) was significantly greater than in control medium (5 ± 1) (P<0.05). These results suggest that oviductal fluid in the estrous bitch maintains sperm motility and induces sperm capacitation. -KEY WORDS: canine, oviductal fluid, sperm capacitation.J. Vet. Med. Sci. 60(2): 197-202, 1998 and four beagles in the estrous period, aged 2-5 years, were used in this experiment. The optimum time for intrauterine insemination in the estrous bitches was determined by daily examination of receptivity for a male dog after vaginal bleeding. Four anestrous bitches ovariohysterectomized at the teaching hospital of our university were used for the zona pellucida-binding assay.Semen collection and evaluation: The sperm-rich second fraction of ejaculated semen [14] was collected by digital manipulation and immediately transported to the laboratory. The concentration of sperm in the semen was determined by hematocytometer counts. The percentage of motile sperm was estimated by using a sperm motility examination plate (Fujihira Industry Inc., Tokyo), and semen samples with good sperm motility (>90%) were used.Intrauterine insemination and flushing of uteri and oviducts: Intrauterine insemination was performed on four estrous bitches on the 4th or 5th day after the start of acceptance, as described in the report by Tsutsui et al. [41]. All of the bitches were laparotomized under halothane inhalation anesthesia. A 20-gauge retaining needle connected to a 1 ml injection syringe was inserted into the middle portion of the right and left uterine horns, and semen adjusted to a concentration of 1 × 10 8 sperm/0.3 ml seminal plasma collected from one of four dogs was...

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Section: Discussionmentioning

confidence: 99%

Induction of Dog Sperm Capacitation by Oviductal Fluid.

Kawakami

Hori

Tsutsui

1998

The Journal of Veterinary Medical Science

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“…The mechanisms of interaction between spermatozoa and the female reproductive tract are of considerable interest, partly because of the potential for understanding the way in which the female reproductive tract maintains sperm viability over such prolonged periods (for review see Hunter & Rodriguez-Martinez 2004). At present there are several indications that sperm storage involves selection, including the observation that the mammalian oviduct preferentially stores uncapacitated spermatozoa (Smith & Yanagimachi 1991, Fazeli et al 1999. In mammals, capacitation is a complex physiological process that involves biochemical, biophysical and metabolic modifications of all parts of the spermatozoon.…”

Section: Capacitation Egg Recognition and Penetrationmentioning

confidence: 99%

Concepts in sperm heterogeneity, sperm selection and sperm competition as biological foundations for laboratory tests of semen quality

Holt

Look²

2004

Reproduction

158

122

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Stringent selection mechanisms, in both internal and external fertilisation systems, reject all but a significant minority of the spermatozoa released at ejaculation. Sperm competition theory provides circumstantial evidence that the selection process involves mechanisms by which the quality of the fertilising spermatozoon is controlled, thereby ensuring that females and their offspring receive high quality genetic material. In this review we examine some of these selection processes to see whether they could be exploited for the improvement of laboratory tests of sperm quality. Such tests are not only required for clinical and agricultural purposes, but are increasingly needed in fields such as reproductive and environmental toxicology where the species requirement is much broader. Despite many years of research, sperm quality assessment methods continue to provide imprecise data about fertility; here we suggest that this may be a consequence of using tests that focus on the spermatozoa that would normally be unable to fertilise under natural conditions. To achieve fertilisation a spermatozoon must be capable of responding appropriately to external signalling stimuli; those involving protein kinase-regulated flagellar function seem especially influential in governing effects ranging from non-Mendelian inheritance in mammals to sperm chemotaxis in sea urchins. Examination of the elicited responses reveals considerable heterogeneity in all species. Here we propose that this level of heterogeneity is meaningful both in terms of understanding how spermatozoa from some individuals possess fertility advantages over spermatozoa from their rivals in sperm competition, and in that the heterogeneity should be exploitable in the development of more accurate laboratory tests.

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“…After deposition of semen in the female reproductive tract during mating, spermatozoa are transported to the oviduct in mammals (Hunter, 1984;Smith and Yanagimachi, 1991). Spermatozoa are sequestered in the oviduct by attachment to oviductal epithelial cells (Hunter, 1981;Hunter and Nichol, 1983), resulting in the formation of a sperm reservoir.…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate mediation of boar sperm binding to oviductal epithelial cells in vitro

Bredl²,

Holt³

et al. 2001

Reproduction

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ResearchAfter mating, mammalian spermatozoa are transported to the lower oviductal isthmus. Spermatozoa are sequestered at the isthmus by attaching and interacting with oviductal epithelial cells, hence forming a sperm reservoir. In several mammalian species, specific carbohydrates mediate spermoviductal epithelial cell binding. A quantitative in vitro free cell bioassay was developed to investigate the involvement of carbohydrate recognition in pig spermoviductal epithelial cell interactions. This assay was validated. The sensitivity of the assay was such that it was possible to discriminate between different sperm concentrations and sperm-oviductal epithelial cell co-incubation periods, spermatozoa with damaged plasma membranes and epithelial cells of non-reproductive origin. Optimal conditions were used to incubate spermatozoa and oviductal epithelial cells in the presence of six hexose sugars at concentrations of 0, 2, 10 and 50 mmol l -1 . A significant (P р 0.05) reduction in the binding of spermatozoa to the oviductal epithelium was detected with 2, 10 and 50 mmol maltose l -1 , 50 mmol lactose l -1 and 50 mmol mannose l -1 . These findings support the hypothesis that attachment of pig spermatozoa to oviductal epithelium before fertilization is mediated by carbohydrate recognition.

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Attachment and release of spermatozoa from the caudal isthmus of the hamster oviduct

Cited by 185 publications

References 13 publications

Induction of Dog Sperm Capacitation by Oviductal Fluid.

Induction of Dog Sperm Capacitation by Oviductal Fluid.

Concepts in sperm heterogeneity, sperm selection and sperm competition as biological foundations for laboratory tests of semen quality

Carbohydrate mediation of boar sperm binding to oviductal epithelial cells in vitro

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