Attachment, Growth and Synthesis by Human Gingival Fibroblasts on Demineralized or Fibronectin‐Treated Normal and Diseased Tooth Roots

Abstract

The purpose of this clinical trial was to evaluate the effect of tetracycline root preparation on guided tissue regeneration in the treatment of Class II furcation defects. Nine pairs of defects with horizontal clinical attachment level value ≥5 mm comprised the study group. Measurements were made to determine presence of plaque, gingival condition, probing depth, and vertical and horizontal clinical attachment level. Defects from each pair were randomly assigned for treatment with an expanded polytetrafluoroethylene membrane (e‐PTFE) and tetracycline root conditioning (100 mg/ml) or the membrane alone. The membranes were removed 4 to 6 weeks postsurgery. Patients were then seen monthly for the duration of the study. Six months postsurgery, all clinical measurements were repeated. Following either treatment, improvement was observed in all clinical parameters, particularly in horizontal clinical attachment level. However, there was not a statistically significant difference in the improvement observed between sites treated with guided tissue regeneration in conjunction with tetracycline as compared to membrane placement alone. Further studies are needed to fully evaluate tetracycline root preparation in conjunction with regenerative therapy. J Periodontol 1993; 64:133–136.

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

“…Fibroblasts are responsible for the normal growth of the organism and are essential in cases where tissue repairs are necessary, being one of the first cells to appear at injured sites. The current concept that both, gingival fibroblasts (15,16) and periodontal ligament fibroblasts (17)(18)(19)(20)(21), have the capacity to regenerate the periodontium has stimulated an interest in the development of clinical methods for the regularization of these cell types (6).…”

Experimental Regeneration of The Periodontium

“…For example, cultured fibroblasts from gingiva have been shown to attach better, spread, and synthesize proteins on partially demineralized and fibronectin-coated surfaces. 41 Cells have been shown to prefer partially demineralized over nondemineralized substrata for attachment and migration. 41 ' 43 This preference may be attributed to the affinity of fibronectin binding to exposed and denatured collagen seen as a result of demineralization.…”

“…41 Cells have been shown to prefer partially demineralized over nondemineralized substrata for attachment and migration. 41 ' 43 This preference may be attributed to the affinity of fibronectin binding to exposed and denatured collagen seen as a result of demineralization. 44 The concept of superficially demineralizing denuded root surfaces to enhance new connective tissue attachment formation has been tested in vivo as well (reviewed by Cole et al).…”

The Effects of the Nd:YAG Laser on in Vitro Fibroblast Attachment to Endotoxin‐Treated Root Surfaces

“…Adelson, et al (1980) observed that fibroblasts did not migrate up inclined planes. Fernyhough and Page (1983) saw that fibroblasts preferentially attached to cracks, crevices and flat surfaces versus vertical surfaces. This problem, however, appeared to be equally present in all groups.…”

“…Scaled and root planed periodontally diseased teeth have been shown to behave similar to non-diseased controls using in vitro fibroblast culture studies (Aleo, et al 1975;Fernyhough and Page, 1983 Their recommendation is to heed the recommendation of the FDA (Becker, 1990 and1991).…”